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6.1.6Plant Nutrition

Environmental Factors and the Rate of Photosynthesis

5 min read 6 parts Lesson 7 of 11 1 quiz

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Section 1 of 6Factors that change the rate

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Effect of Limiting Factors on the Rate of Photosynthesis — 2D Simulation
Effect of Light Intensity on Rate of Photosynthesis — 2D Simulation
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r054-enzyme-concentration
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r052-substrate-concentration-rate
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Rate of photosynthesis
Light intensity
  • Light intensity Light provides the energy for photosynthesis, so increasing light intensity increases the rate, until another factor becomes limiting.
Carbon dioxide concentration
  • Carbon dioxide concentration Carbon dioxide is a raw material, so a higher concentration increases the rate, until something else limits it.
Temperature
  • Temperature Photosynthesis is controlled by enzymes.
    • Warming increases the rate because molecules have more kinetic energy and collide more often.
Too hot
  • Too hot Above the optimum temperature the enzymes are denatured and the rate falls.

Section 2 of 6Method: counting bubbles from pondweed

1
Place a piece of pondweed such as Elodea or Cabomba in a beaker of water, with the cut end upwards.
2
Set up a lamp at a measured distance from the beaker and allow the plant to adjust for a few minutes.
3
Count the bubbles of oxygen released from the cut stem in one minute.
4
Repeat the count to calculate a mean, then change the independent variable and repeat.
5
For greater accuracy, collect the oxygen in a gas syringe or an inverted tube over a longer time and measure its volume.

Section 3 of 6Varying each factor

FactorHow it is variedControl needed
Light intensityChange the distance of the lamp from the beakerPlace a glass tank of water between lamp and plant to absorb heat so the temperature does not change
TemperatureUse a water bath set to different temperaturesSame lamp distance and same carbon dioxide level
Carbon dioxide concentrationDissolve different amounts of sodium hydrogen carbonate in the waterSame temperature and light

Section 4 of 6Interpreting the graphs

Investigating temperature — Interactive
Investigating the Effect of Temperature on Photosynthesis in an Aquatic Plant
Effect of temperature on rate of photosynthesis (graph)
Light intensity limiting photosynthesis (graph)
Effect of light intensity on rate of photosynthesis (graph)
Effect of carbon dioxide concentration on rate of photosynthesis (graph)
Carbon dioxide concentration limiting photosynthesis (graph)
Effect of temperature on rate of photosynthesis (graph)
Effect of temperature on rate of respiration (graph)
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Effect of temperature on rate of respiration (graph)
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response time graph
Investigating the Effect of Light Distance on Photosynthesis — Full 2D Simulation
Investigating Photosynthesis — 2D Simulation
Bright Light — Interactive Biology Diagram
Bright Light — Interactive Biology Diagram
Bright Light — Interactive Biology Diagram
rising line graph
bell curve graph
Effect of pH on enzyme activity (graph)
Photosynthesis summary: carbon dioxide, water, light to glucose and oxygen
Graph of carbon dioxide concentration against plant biomass
1Rate rises steeply
  • Rate rises steeply At first the factor being increased is limiting, so the rate increases with it.
2Line begins to level
  • Line begins to level Another factor becomes limiting.
3Plateau
  • Plateau Adding more of the first factor makes no difference;
    • the rate is controlled by a different factor, such as temperature or carbon dioxide.
4Temperature curve falls
  • Temperature curve falls At high temperature the enzymes denature, so the rate decreases.

Section 5 of 6Key terms

TermMeaning
Rate of photosynthesisHow quickly a plant makes glucose, measured here by oxygen released per unit time.
Limiting factorThe factor in shortest supply, which limits the rate of a process.
Light intensityA measure of how bright the light is; it decreases as distance from the source increases.
Sodium hydrogen carbonateA compound that dissolves in water to provide carbon dioxide for the plant.
Aquatic plantA plant that grows in water, such as Elodea, used because its oxygen bubbles can be seen.

Section 6 of 6Exam technique

1
Saying that raising temperature always increases photosynthesis; beyond the optimum, enzymes denature and the rate drops.
2
Not controlling temperature when changing light distance, because the lamp also heats the water.
3
Counting bubbles for too short a time or only once, which gives unreliable results.
4
Assuming the graph levels off because the plant has 'run out of light', when another factor has become limiting.
5
Forgetting to explain that bubbles of gas are oxygen, a product of photosynthesis.
Biology 0610 & 9700 Exam Model Limiting Factors Master

Photosynthesis: Limiting Factors & Graph Plateau

6CO2 + 6H2O → C6H12O6 + 6O2 (Light & Chlorophyll required)

  • Limiting Factor Definition: Something present in the environment in such short supply that it restricts life processes.
  • Graph Plateau: When rate levels off as light intensity increases, light is no longer the limiting factor; CO2 concentration or temperature has become limiting.
  • High Temperature Drop: Beyond optimum temp (~40°C), enzymes (e.g. RuBisCO) denature, permanently decreasing rate.