4.2Topic 4 · Biodiversity and Natural Resources
Resources from Plants — Edexcel A Level Biology A (SNAB) 9BI0 Revision Notes & Topical Questions
30 min read 27 parts Lesson 11 of 22 1 quiz
Resources from Plants Edexcel A Level Biology SNAB notes: Describe the ultrastructure of plant cells and the functions of their organelles, and compare them with animal cells.Area of clear zone = πr2 (r = radius = diameter ÷ 2); units mm2 or cm2.Tensile strength investigationadd …
- Starch test
- Lignin test
- Plant cell structures not in animal cells
- Cell wall arrangement
- Xylem vessels
- Sclerenchyma fibres
Section 1 of 27Resources from Plants
- Deficiency causes symptoms such as yellow leaves or stunted growth.
- Cover tubes with foil to stop algal growth, give equal light, then record mass change and visible symptoms after several days.
Section 2 of 27Process & sequence
- then filter; leave to evaporate ethanol/control uses ethanol only.
Tensile strength of plant fibreswrite these steps in this order; each step is a marking point.
Section 3 of 27Compare
| Feature | Starch | Cellulose |
|---|---|---|
| Monomer | α-glucose | β-glucose |
| Bonds | 1,4 (amylose); 1,4 and 1,6 (amylopectin) | 1,4 only; alternate glucose inverted |
| Shape | Helix (amylose), branched (amylopectin) | Straight, unbranched chains |
| H-bonds between chains | Few | Many, forming microfibrils |
| Function | Energy store (compact, insoluble) | Structural: cell wall strength |
| Location | Amyloplasts/chloroplasts | Cell wall |
| Stage | Withering (digitalis, 1700s) | Modern protocol |
|---|---|---|
| Origin | Folk remedy (foxglove) for dropsy | Candidate from research/plants |
| Early testing | Tried on patients directly | Pre-clinical: cells, tissues, animals |
| Dose | Trial and error, recorded effects | Phase 1: small group healthy volunteers, safety and dosage |
| Efficacy | Observed in patients | Phase 2: small group of patients; Phase 3: large double-blind, placebo-controlled randomised trial |
Section 4 of 27Worked examples
- r = 14 ÷ 2 = 7 mm. Area = π × 72 = 3.142 × 49 = 154 mm2 (3 s.f.).
A fibre broke when a total mass of 850 g was hung from it. Calculate the breaking force.
- Mass = 0.850 kg. Force = 0.850 × 9.81 = 8.34 N (3 s.f.).
- Area A = π × 102 = 314 mm2; Area B = π × 52 = 78.5 mm2. Ratio = 314 ÷ 78.5 = 4.0 times (area scales with r2).
Section 5 of 27Keep going
Resources from Plants Edexcel A Level Biology SNAB: what to do next
These Resources from Plants Edexcel A Level Biology SNAB notes covered the key ideas, worked examples and exam technique for this topic. Check your Resources from Plants Edexcel A Level Biology SNAB understanding with the practice questions below, then reread any Resources from Plants Edexcel A Level Biology SNAB point you were unsure about before moving on.
Resources from Plants Edexcel A Level Biology SNAB: revision checklist
Section 6 of 27Plant cell organelles
Plant cells contain most of the organelles found in animal cells, plus a few found only in plants.
- The cell wall is formed outside the cell membrane and gives structural support through the polysaccharide cellulose.
- Plasmodesmata are gaps in the wall lined with membrane that allow transport and communication between cells.
- Chloroplasts are larger than mitochondria and are also surrounded by a double membrane.
- Membrane-bound compartments called thylakoids containing chlorophyll stack to form grana (singular granum).
- Amyloplasts are small membrane-bound organelles containing starch granules;
- they store starch and convert it back to glucose when needed.
- and gives support when full (turgor).
Section 7 of 27Xylem and sclerenchyma
Xylem is vascular tissue that transports water and dissolved minerals up the plant and also provides support.
- which makes them waterproof and strong,
- so they withstand the pressure created by the moving column of water.
Section 8 of 27Phloem
Phloem is a complex tissue made up of several cell types; its bulk is sieve tube elements, the main conducting cells, and companion cells.
- so there is space for mass flow of sucrose and amino acids.
Section 9 of 27Vascular bundles
Vascular bundles consist of xylem vessels grouped together with phloem tissue (and often sclerenchyma).
- and sclerenchyma fibres cap the bundle for support.
- and have companion cells containing a nucleus and cytoplasm.
| Feature | Xylem vessel | Sieve tube element |
|---|---|---|
| Living? | Dead | Alive |
| Wall | Lignified | Cellulose, no lignin |
| End walls | Absent | Sieve plates |
| Carries | Water and minerals | Sucrose and amino acids |
Section 10 of 27Worked examples
Explain how the structure of xylem vessels is related to their function. [4]4 marks
They are dead, hollow tubes with no end walls so water can flow continuously; lignified walls make them waterproof and strong enough to withstand tension and pressure; pits allow water to move sideways.
Describe the role of a companion cell. [2]2 marks
It controls the metabolism of the sieve tube element and loads and unloads sugars into it, using ATP from many mitochondria.
Distinguish sclerenchyma fibres from sieve tubes. [2]2 marks
Sclerenchyma are dead and lignified for support; sieve tubes are living and transport sugars.
Section 11 of 27Water and inorganic ions
Plant cells carry out many functions, and to do so efficiently the plant needs water and inorganic ions (minerals). Water is needed for photosynthesis, turgor and transport. Magnesium ions are needed to make chlorophyll; a lack causes chlorosis (yellowing of leaves). Nitrate ions provide nitrogen for amino acids, proteins, nucleotides and chlorophyll; a lack causes stunted growth and yellow older leaves. Calcium ions form calcium pectate in the middle lamella between cell walls; phosphate ions are needed for ATP, DNA, RNA and phospholipids.
Practical: mineral deficiency. Take nine seedlings from the same plant of the same age and record the mass of each.
- and one distilled water control, with roots in the solution.
Section 12 of 27Starch
Starch is the storage polysaccharide of plants, stored as granules in plastids (including chloroplasts) and amyloplasts.
- which is branched with 1,6 bonds as well.
- and can be hydrolysed to release glucose.
Section 13 of 27Cellulose
Cellulose is a polymer of beta-glucose forming long, unbranched chains.
- which are grouped into microfibrils and fibres,
- giving cellulose its great strength.
Section 14 of 27Plant fibres
Sclerenchyma fibres and xylem vessels are made of long, hollow plant cells.
- which makes it almost impossible for substances to enter or leave the cell.
Practical: identifying tissues in stems. A permanent pre-prepared slide may be used, or a thin section of stem is cut, stained (for example phloroglucinol for lignin, which stains red, or toluidine blue) and mounted as a temporary slide, then the tissues are identified from their positions and staining.
Section 15 of 27Worked examples
Explain why a lack of magnesium causes yellow leaves. [2]2 marks
Magnesium is part of chlorophyll, so with less of it less chlorophyll is made, and leaves appear yellow (chlorosis).
Describe how the structure of cellulose gives strength. [3]3 marks
Long unbranched chains of beta-glucose with alternate units inverted; many hydrogen bonds between neighbouring chains form microfibrils, giving great tensile strength.
Give two ways in which starch is suited to storage. [2]2 marks
It is compact and insoluble, so it does not alter water potential, and it can be hydrolysed to glucose.
Section 16 of 27Practical: tensile strength of plant fibres
The tensile strength of a fibre is the maximum load it can carry before breaking; this matters, for example, in choosing fibres for ropes.
- and add masses one at a time until the fibre breaks.
- (e.g. 100 g masses)
Section 17 of 27Development of drug testing
New drugs must be tested for effectiveness and side effects before being sold.
- then animals,
- then human trials in phases: phase 1 on a small number of healthy volunteers to test safety;
- phase 2 on a small group of patients to test effectiveness and dosage, using a placebo;
- phase 3 on a larger number of patients to confirm effectiveness and detect side effects.
- which looks exactly like the drug but contains no active ingredient.
Section 18 of 27Practical: antimicrobial properties of plants
Bacteria are microscopic prokaryotes (cells with no nucleus or membrane-bound organelles), often used to test antimicrobial substances.
- then spread evenly with a sterile plastic spreader.
- and incubate at about 25 °C for 24 to 48 hours.
- (not 37 °C, to avoid growing human pathogens)
The area around each disc where bacteria cannot grow is the clear zone. The larger the clear zone, the more effective the antimicrobial properties of the extract.
Section 19 of 27Worked examples
Explain the purpose of a placebo. [2]2 marks
To show that any improvement is due to the drug and not to patients' expectations.
Why is a disc soaked in ethanol included? [2]2 marks
As a control, to show that the clear zone is due to the plant extract and not the ethanol or the disc.
Why should agar plates be incubated at 25 degrees rather than 37 degrees? [1]1 mark
To reduce the chance of growing pathogens that thrive at body temperature.
Section 20 of 27Why aseptic technique matters
Contamination of the culture by other microorganisms or human pathogens from outside has a negative impact on the growth of the bacteria under investigation.
- where updraught carries microbes away;
- and keeping the lid of the petri dish on as much as possible.
Section 21 of 27Controls and results
One paper disc must be soaked in ethanol instead of plant extract. This ensures that any differences in bacterial growth are attributed to the antimicrobial properties of the plant extracts and not to other factors such as the paper discs themselves or the ethanol.
Record the diameter of each clear zone to the nearest whole millimetre.
Section 22 of 27Sustainability and plant materials
Sustainability means using resources in a way that meets the requirements of the current generation without depleting them for future generations.
- or fast-growing crops.
- and reduces reliance on non-renewable resources such as oil.
Section 23 of 27Interpreting an antimicrobial plate
Suppose three discs show clear zones of diameter 10, 16 and 0 mm, with ethanol giving 0 mm.
- since area is proportional to the square of the radius (201 mm2 compared with 79 mm2 for the 10 mm zone).
Section 24 of 27Worked examples
Name two aseptic techniques. [2]2 marks
Flaming the loop and the neck of the bottle; disinfecting the bench; closing doors and windows.
Calculate the area of a clear zone of diameter 12 mm. [2]2 marks
r = 6 mm; area = 3.14 x 36 = 113 mm2.
Define sustainability. [1]1 mark
Meeting the needs of the present generation without compromising those of future generations.
Section 25 of 27Key terms
| Term | Meaning |
|---|---|
| Lignin | Waterproof, strong polymer in xylem and fibre walls. |
| Plasmodesmata | Channels through plant cell walls linking cells. |
| Sieve plate | Perforated end wall of a sieve tube element. |
| Companion cell | Cell that supports a sieve tube element. |
| Tonoplast | Membrane around the vacuole. |
| Vascular bundle | Group of xylem and phloem vessels. |
| Term | Meaning |
|---|---|
| Amylose | Unbranched helical polymer of alpha-glucose in starch. |
| Amylopectin | Branched polymer of alpha-glucose in starch. |
| Cellulose | Polymer of beta-glucose forming plant cell wall. |
| Chlorosis | Yellowing from lack of chlorophyll. |
| Microfibril | Bundle of cellulose chains. |
| Term | Meaning |
|---|---|
| Tensile strength | Maximum load a material can bear before breaking. |
| Placebo | Treatment with no active ingredient. |
| Clinical trial | Test of a drug on humans. |
| Clear zone | Area around a disc where bacterial growth is inhibited. |
| Agar | Jelly used as a bacterial growth medium. |
| Term | Meaning |
|---|---|
| Aseptic technique | Methods that prevent contamination of cultures. |
| Sustainability | Use of resources without depleting them for the future. |
| Renewable resource | Resource that is replaced naturally as it is used. |
| Autoclave | Pressure steriliser. |
Section 26 of 27Command terms
Section 27 of 27Exam technique
Marked practiceQuizzes for this lesson
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