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2.5Structures and Functions in Living Organisms

Nutrition — Edexcel Modular - OL Revision Notes & Topical Questions

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Nutrition Edexcel O Level Modular Biology notes: understand the process of photosynthesis and its importance in the conversion of light energy to chemical energyStomach acid kills microbes and gives pepsin its optimum pH.Villi have thin walls, capillaries and lacteals.Large intes…

  • Nutrition Edexcel O Level Modular Biology: what to do next
  • Mechanical digestion
  • Chemical digestion
  • Give / State / Name
  • Evaluate / Assess
  • Keywords the mark scheme looks for in this lesson

Section 1 of 30Process & sequence

  • Mouth
  • Oesophagus (peristalsis)
  • Stomach
  • Small intestine (absorption)
  • Large intestine (water)
  • Rectum, anus
Nutrients
Carbohydrates – energy
Proteins – growth
Lipids – energy store
Vitamins, minerals, fibre, water

Section 2 of 30Keep going

Nutrition Edexcel O Level Modular Biology: what to do next

These Nutrition Edexcel O Level Modular Biology notes covered the key ideas, worked examples and exam technique for this topic. Check your Nutrition Edexcel O Level Modular Biology understanding with the practice questions below, then reread any Nutrition Edexcel O Level Modular Biology point you were unsure about before moving on.

Nutrition Edexcel O Level Modular Biology revision notes

Section 3 of 30Leaf adaptations for photosynthesis

The leaf is adapted to absorb light and carbon dioxide.

Leaf adaptations for photosynthesis
The palisade cell layer at the top maximises light absorption because the cells are packed with chloroplasts and sit close to the surface.
The spongy mesophyll beneath contains air spaces that allow carbon dioxide to diffuse through the leaf, giving a large surface area for exchange.
Stomata open to let carbon dioxide in and oxygen out; veins bring water and remove sucrose; the thin, flat shape gives a short diffusion distance and large area for light.

Section 4 of 30Plants make more than carbohydrates

Photosynthesis provides carbohydrates, but plants also require proteins, lipids and nucleic acids (DNA).

Because plants do not eat, they must make these themselves.
Carbohydrates contain carbon, hydrogen and oxygen, while proteins also contain nitrogen.
Nitrate
Two essential mineral ions are nitrate, to make amino acids and proteins, and magnesium, to make chlorophyll.
Without them plants cannot photosynthesise or grow properly.
DeficiencySymptomReason
NitrateStunted growth, older leaves yellowCannot make enough amino acids and proteins
MagnesiumLeaves yellow (chlorosis), especially between veinsCannot make chlorophyll

Section 5 of 30Practical: oxygen produced by pondweed

1
Place a piece of pondweed with a cut end upward in a beaker of water containing sodium hydrogencarbonate (a source of carbon dioxide).
2
Put a lamp at a measured distance and wait for the plant to adjust.
3
Count the bubbles released from the cut end in a set time (e.g. one minute).
4
Change the distance of the lamp and repeat.

As photosynthesis occurs, oxygen gas produced is released as bubbles from the cut end. More bubbles mean a faster rate. A heat shield (water between the lamp and plant) prevents temperature changes. Rate falls as the lamp moves away because light intensity falls (inverse square relationship).

Section 6 of 30Why leaves are tested for starch rather than glucose

Although plants synthesise glucose, the glucose is quickly used up, converted into other substances and transported or stored as starch. Starch is stored in the chloroplasts where photosynthesis occurs, so testing a leaf for starch is a reliable indicator of which parts of the leaf are photosynthesising.

Section 7 of 30Photosynthesis, respiration and gas exchange in leaves

During the day a plant photosynthesises faster than it respires, so there is a net uptake of carbon dioxide and a net release of oxygen.

Photosynthesis, respiration and gas exchange in leaves
At night only respiration occurs, so there is a net release of carbon dioxide.
At the compensation point, the rates of photosynthesis and respiration are equal and there is no net gas exchange; this can be shown with hydrogencarbonate indicator,
  • which is red at atmospheric carbon dioxide level, yellow when carbon dioxide is high and purple when low (photosynthesis exceeds respiration).
    • (respiration only)
Tube conditionsIndicator colourExplanation
Leaf in dark (foil-wrapped)YellowOnly respiration, so carbon dioxide builds up
Leaf in bright lightPurplePhotosynthesis uses more carbon dioxide than is produced
Leaf in moderate lightRedRates balanced

Uses of photosynthetic products and importance: photosynthesis is the source of food energy for almost all food chains, and the source of the oxygen in the atmosphere.

Photosynthesis, respiration and gas exchange in leaves
Plants convert some glucose to sucrose for transport in phloem, to starch for storage in tubers and seeds, and to cellulose for cell walls.
Using nitrate from the soil plants also make amino acids, which they join to make proteins such as enzymes.

Section 8 of 30Compare

Digestive enzymes
EnzymeMade inActs inSubstrateProductOptimum pH
Salivary amylaseSalivary glandsMouthStarchMaltose7
PepsinStomachStomachProteinPeptides2
Pancreatic amylasePancreasSmall intestineStarchMaltose8
TrypsinPancreasSmall intestineProteinPeptides8
LipasePancreasSmall intestineLipidsFatty acids + glycerol8
MaltaseSmall intestine wallSmall intestineMaltoseGlucose8

Section 9 of 30Worked examples

  1. Explain how the palisade layer is adapted for photosynthesis. [2]2 marks

  2. Explain why magnesium deficiency makes leaves yellow. [2]2 marks

  3. Describe how to measure the rate of photosynthesis using pondweed. [3]3 marks

  4. Why test for starch instead of glucose? [1]1 mark

Section 10 of 30Investigating the need for light

1
De-starch the plant by keeping it in a dark cupboard for 24 hours so no starch remains in the leaves.
2
Cover part of one leaf with aluminium foil (or a black card with a stencil) and place the plant in bright light.
3
Remove the leaf, boil it in water for about a minute to kill it and soften it.
4
Heat it in ethanol in a water bath until the chlorophyll is removed.
  • Ethanol is flammable, so turn off the Bunsen burner;
    • use an electric water bath or water from a just-boiled kettle.
5
Rinse in water, spread on a white tile and cover with iodine solution.

Result

  • the area exposed to light turns blue-black (starch present);
    • the covered area stays yellow-brown (no starch), so light is necessary for photosynthesis.

Section 11 of 30Investigating the need for carbon dioxide

After de-starching, enclose one leaf in a conical flask containing potassium hydroxide (which absorbs carbon dioxide) and another leaf in a control flask without it, then leave in light.

Investigating the need for carbon dioxide
After the starch test, the leaf from the potassium hydroxide flask remains yellow-brown,
  • as it could not photosynthesise; the control leaf turns blue-black.
CORMS: C - change whether carbon dioxide is present; O - same species, age and size of leaf; R - repeat; M - observe colour change with iodine; S - ethanol safety.

Section 12 of 30Balanced diet and malnutrition

A balanced diet consists of all the food groups in the correct proportions. An unbalanced diet can lead to malnutrition, which causes health problems such as obesity (too much energy), kwashiorkor (too little protein), scurvy (vitamin C), rickets (vitamin D or calcium) and anaemia (iron).

NutrientSourcesFunction
CarbohydrateBread, cereals, pasta, rice, potatoesEnergy
ProteinMeat, fish, dairy, pulsesGrowth and repair
LipidOils, butterEnergy store, insulation
FibreVegetables, wholegrainProvides bulk (roughage) to push food through the intestine
Vitamins and mineralsFruit, vegetables, meat, dairyNeeded in small quantities to maintain health

Dietary needs vary

  • more active people need more energy;
    • children need a higher proportion of protein for growth;
    • pregnant women need extra energy, calcium and iron to support the fetus.

Section 13 of 30Nutritional data: energy requirements and disorders

Energy requirements depend on age, sex, activity and size.

Nutritional data: energy requirements and disorders
Approximate daily needs: a teenage boy about 11 000 kJ, a teenage girl about 9000 kJ, an adult office worker about 10 000 kJ, a manual worker about 14 000 kJ, a pregnant woman an additional amount of about 800 kJ.
Eating more energy than is used leads to obesity, linked to type 2 diabetes, heart disease and high blood pressure; eating too little leads to weight loss and starvation.
Food labels show energy in kJ per 100 g so the energy in a portion can be calculated by multiplying by the mass/100.
DisorderCauseSymptoms
ScurvyLack of vitamin CBleeding gums, slow healing
RicketsLack of vitamin D or calciumSoft, bowed bones in children
AnaemiaLack of ironPale, tired, short of breath
KwashiorkorLack of proteinSwollen abdomen, poor growth

Tests for food types

  • Benedict's for reducing sugars, iodine for starch, biuret for protein and ethanol emulsion for lipids;
    • DCPIP for vitamin C, which is decolourised faster by foods with more vitamin C.

Section 14 of 30Worked examples

  1. Describe why a leaf is boiled in ethanol. [2]2 marks

  2. Explain why the covered part of a leaf stays yellow-brown. [2]2 marks

  3. Why do children need more protein per kilogram than adults? [1]1 mark

  4. Suggest why a plant is de-starched. [1]1 mark

Section 15 of 30Organs of the alimentary canal

The digestive system is an organ system. Digestion breaks large insoluble molecules such as starch and proteins into smaller soluble ones that can be absorbed into the blood and delivered to cells. The alimentary canal and accessory organs work together.

OrganRole
Mouth and salivary glandsTeeth chew food (mechanical digestion, increasing surface area); salivary amylase starts starch digestion
OesophagusPeristalsis pushes the bolus to the stomach
StomachChurning; pepsin (protease); hydrochloric acid kills bacteria and gives low pH
Liver and gall bladderMake and store bile
PancreasMakes digestive enzymes and the hormone insulin; food does not pass through it
Small intestine (duodenum, ileum)Digestion completed; absorption; pH 8-9
Large intestineAbsorbs water; forms faeces

Section 16 of 30Peristalsis and chemical digestion

Peristalsis is a wave of contraction of circular and longitudinal muscles in the gut wall that pushes food along. Once in the stomach the food is churned into chyme. Mechanical digestion (chewing, churning, emulsification) breaks food into smaller pieces and increases surface area. Chemical digestion mainly takes place by enzymes, which break bonds in large molecules.

Peristalsis and chemical digestion
Starch is broken down by amylase to maltose, then maltase to glucose.
Proteins are broken down by pepsin in the stomach into smaller polypeptides, then by proteases from the pancreas and small intestine into amino acids.
Lipids are broken down by lipase into glycerol and fatty acids.

Section 17 of 30Bile

Bile is an alkaline fluid produced by the liver and stored in the gall bladder.

Bile
It is released into the small intestine, where it: (1) neutralises the hydrochloric acid from the stomach,
  • giving the alkaline pH needed for pancreatic enzymes, and (2) emulsifies lipids, breaking large drops into smaller ones with a larger surface area,
  • so lipase can digest them faster.
The molecules responsible are bile salts.
Emulsification is mechanical digestion, like tearing paper into smaller pieces: no bonds are broken.

Section 18 of 30Small intestine: structure and adaptations

Absorption is the movement of small digested molecules from the gut into the blood (glucose and amino acids) and lymph (fatty acids and glycerol).

Small intestine: structure and adaptations
It occurs by diffusion and active transport; water is absorbed by osmosis.
The lining has villi with a large surface area, thin walls (one cell thick), a good blood supply to maintain gradients, a lacteal for fats,
  • and microvilli on the cells.
Exam answers on villi need linking the structure to the function.

Section 19 of 30Tests, adaptations and extended-answer structure

For a six-mark question describing digestion, structure your answer along the gut: mouth (amylase, chewing), stomach (pepsin, acid), small intestine (bile, lipase, amylase, protease, maltase, absorption), large intestine (water absorption).

Tests, adaptations and extended-answer structure
Link each organ to what is digested and what the products are.
Common error: stating that bile digests fat; the correct idea is that bile emulsifies fat to give a larger surface area,
  • and the digestion is done by lipase.

Surface area calculations

  • if a villus is modelled as a cylinder of radius 0.1 mm and height 1 mm, its curved surface area is 2 x pi x 0.1 x 1 = 0.63 mm2;
    • with millions of villi the total absorbing area is hundreds of times that of a smooth tube.

1
Each villus has a one-cell-thick epithelium, a capillary network and a lacteal.
2
The ileum is also lined with microvilli on each cell, increasing the surface further.
3
Water is absorbed in the small intestine and the colon, and the remaining material forms faeces, stored in the rectum and egested through the anus.
4
Lactose intolerance results from lack of lactase,
  • so lactose passes undigested to the large intestine where bacteria ferment it, causing gas and diarrhoea.
5
Teeth: incisors cut, canines tear, premolars and molars grind; dental decay results from acid produced by bacteria feeding on sugar.

Section 20 of 30Worked examples

  1. Name the enzymes that digest starch to glucose. [2]2 marks

  2. State two functions of bile. [2]2 marks

  3. Explain why emulsification speeds up fat digestion. [2]2 marks

  4. Describe peristalsis. [2]2 marks

Section 21 of 30The principle

Food stores chemical energy. When it is burned, this energy is transferred as heat to surrounding water. The larger the increase in water temperature, the more energy was stored in the food. A food sample burned under a boiling tube of water is a simple calorimeter.

Section 22 of 30Method

1
Measure 25 cm3 of water with a measuring cylinder and pour it into a boiling tube clamped in a stand.
2
Record the starting temperature of the water.
3
Weigh the food sample (e.g. a peanut or piece of bread) and mount it on a mounted needle.
4
Light the food in a Bunsen flame, then hold it under the tube.
5
When it stops burning, relight until it no longer lights.
6
Stir and record the highest temperature of the water.
7
Repeat with other foods using fresh water at the same starting temperature.

Section 23 of 30Calculating energy

Calculating energy
Energy transferred (J) = mass of water (g) x temperature rise (C) x 4.2 (J/g/C).
Energy per gram of food (J/g) = energy transferred / mass of food burned (g).
Worked example: 25 g water rises by 20 C when 0.5 g of food is burned. Energy = 25 x 20 x 4.2 = 2100 J. Energy per gram = 2100 / 0.5 = 4200 J/g.

Section 24 of 30Evaluation

ProblemEffectImprovement
Heat lost to the airResults too lowUse a draught shield, insulate the tube, keep the food close
Incomplete combustionLess energy transferredRelight the sample until it will not burn
Different starting temperaturesUnfair comparisonStart each test at the same temperature
Small mass of foodLarge percentage errorsUse a larger mass and repeat

Compare foods using energy per gram. Fats give about 37 kJ/g, carbohydrates and proteins about 17 kJ/g, so high-fat foods give the highest values. Variables to control: volume of water, distance between flame and tube, mass of food.

Section 25 of 30Planning the practical with CORMS

C - changethe type of food (peanut, crisps, bread, cheese).

1
O - organism: not relevant here because the material is food, but the same type of container and water are used.
2
R - repeat: burn each food at least three times and calculate a mean energy value, ignoring outliers.
3
M - measure: temperature rise of water with a thermometer to 0.5 C, mass of food with a balance to 0.01 g, volume of water with a measuring cylinder.
4
S - same: volume of water, starting temperature, distance between flame and tube, size of tube and food mass.
5
Safety: wear eye protection, tie back long hair, keep flammable material away, use tongs and a heatproof mat, and allow burnt samples to cool.

Accuracy and precisionthe mean energy value from this method is always lower than the value on the packet because heat is lost.

Planning the practical with CORMS
A bomb calorimeter, in which the food is burned in pure oxygen inside a sealed insulated container, gives a more accurate result.
To compare foods, calculate energy per gram (J/g) from the data, then express as kJ/g by dividing by 1000.
  • For example, a peanut at 24 kJ/g contains much more energy than bread at 10 kJ/g, because it contains more fat.
Check results by comparing with the known values: fat 37 kJ/g, protein and carbohydrate 17 kJ/g.

Section 26 of 30Worked examples

  1. Calculate the energy transferred when 25 g water rises by 16 C. [2]2 marks

  2. 0.8 g of crisps heat 25 g of water by 10 C. Calculate energy per gram. [3]3 marks

  3. Give two reasons why the result is lower than the true value. [2]2 marks

Section 27 of 30Key terms

TermMeaning
Palisade layerLeaf layer with many chloroplasts at the top.
Spongy mesophyllLeaf layer with air spaces.
ChlorosisYellowing of leaves.
NitrateMineral ion needed for amino acids.
MagnesiumMineral ion needed for chlorophyll.
TermMeaning
De-starchRemove starch from leaves by keeping a plant in the dark.
Potassium hydroxideA chemical that absorbs carbon dioxide.
Balanced dietA diet containing all nutrients in correct proportions.
MalnutritionPoor health from an unbalanced diet.
FibreIndigestible plant material giving bulk.
TermMeaning
PeristalsisMuscular waves moving food along the gut.
ChymeSemi-liquid mixture of food and stomach secretions.
BileAlkaline fluid from the liver that emulsifies fat.
VilliFinger-like projections increasing absorption area.
PepsinProtease from the stomach.
LipaseEnzyme that digests fats.
TermMeaning
CalorimetryMeasuring energy transfers, e.g. from burning food.
Specific heat capacity of water4.2 J/g/C.
CombustionBurning.
Draught shieldA screen used to reduce heat loss.

Section 28 of 30Syllabus checklist

1
understand the process of photosynthesis and its importance in the conversion of light energy to chemical energy
2
know the word equation and the balanced chemical symbol equation for photosynthesis
3
understand how varying carbon dioxide concentration, light intensity and temperature affect the rate of photosynthesis
4
describe the structure of the leaf and explain how it is adapted for photosynthesis
5
understand that plants require mineral ions for growth, and that magnesium ions are needed for chlorophyll and nitrate ions are needed for amino acids
6
practical: investigate photosynthesis, showing the evolution of oxygen from a water plant, the production of starch and the requirements of light, carbon dioxide and chlorophyll
7
understand that a balanced diet should include appropriate proportions of carbohydrate, protein, lipid, vitamins, minerals, water and dietary fibre
8
identify the sources and describe the functions of carbohydrate, protein, lipid (fats and oils), vitamins A, C and D, the mineral ions calcium and iron, water and dietary fibre as components of the diet
9
understand how energy requirements vary with activity levels, age and pregnancy
10
describe the structure and function of the human alimentary canal, including the mouth, oesophagus, stomach, small intestine (duodenum and ileum), large intestine (colon and rectum) and pancreas
11
understand how food is moved through the gut by peristalsis
12
understand the role of digestive enzymes, including the digestion of starch to glucose by amylase and maltase, the digestion of proteins to amino acids by proteases and the digestion of lipids to fatty acids and glycerol by lipases
13
understand that bile is produced by the liver and stored in the gall bladder
14
understand the role of bile in neutralising stomach acid and emulsifying lipids
15
understand how the small intestine is adapted for absorption, including the structure of a villus

Section 29 of 30Command terms

Command terms
Give / State / Name
Recall a fact; one idea per mark.
Define
Give the exact meaning using the specification keywords.
Describe
Give an account of what happens / the pattern in data, quoting values; no reasons needed.
Explain
Give reasons with linked statements (cause → effect). Each linked step is usually a separate marking point.
Suggest
Use knowledge in a new context; more than one answer may be accepted.
Compare
Similarities and differences; each point must refer to both items.
Calculate
Show working; include units; check significant figures. Method marks are given even if the final answer is wrong.
Discuss
Explore the issue from different angles and link the points.
Evaluate / Assess
Weigh strengths and weaknesses and reach a supported judgement.
Deduce
Draw a conclusion from the information given.
Justify
Support a statement/choice with evidence.

Section 30 of 30Exam technique

1
Saying nitrate is for chlorophyll.
2
Saying stomata are in the upper surface mostly.
3
Forgetting a carbon dioxide source in water.
4
Saying bubbles are carbon dioxide.
1
Heating ethanol in a flame.
2
Not de-starching.
3
Saying fibre provides energy.
4
Saying pregnant women need less iron.
1
Saying bile contains lipase.
2
Saying food passes through the pancreas.
3
Saying absorption happens in the stomach mainly.
4
Saying emulsification breaks chemical bonds.
1
Using volume of food instead of mass.
2
Forgetting to divide by the mass of food.
3
Starting different tests at different temperatures.
4
Not re-lighting the food.

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