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Unit 4: Energy, Environment, Microbiology and Immunity

Unit 4 — Complete A Level and AS source — IAL Edexcel Modular Revision Notes & Topical Questions

5 min read 9 parts Lesson 2 of 8 Unit quiz

📚 IAL Edexcel Modular Hub / Topical Revision
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Curriculum Equation Key قوانين الرقم الهيدروجيني والاتزان الأيوني - pH & Acid-Base Equilibrium FormulasFast Exam Solution
pH = -log[H⁺] | pOH = -log[OH⁻] | pH + pOH = 14 | [H⁺][OH⁻] = 10⁻¹⁴
Word Equation & Conditions: الرقم الهيدروجيني = -لوغاريتم تركيز أيون الهيدروجين | pH + pOH = 14
Location & Specifications: الاتزان الأيوني، كيمياء 3 ثانوي، التحصيلي، و AP Chemistry.
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Lesson summary

Unit 4 — Complete A Level and AS source notes — IAL EDEXCEL MODULAR notes: Summarise photosynthesis, ecosystems, climate change, microbiology, immunity and forensics.Overall equation — 6CO2 + 6H2O → C6H12O6 + 6O2 in light with chlorophyll.Light-dependent reactions — in the thylak…

  • Light-independent reactions (Calvin cycle)
  • Trophic level
  • Energy transfer
  • Succession
  • Population estimates
  • Abiotic measurements

Part 1 of 7Photosynthesis

1
Overall equation
  • 6CO2 + 6H2O → C6H12O6 + 6O2 in light with chlorophyll.
2
Light-dependent reactions
  • Light-dependent reactions in the thylakoid membranes light energy splits water (photolysis),
    • and the released electrons pass along an electron transport chain.
  • Protons are pumped to make ATP by chemiosmosis, and NADP is reduced.
3
Light-independent reactions (Calvin cycle)
  • Light-independent reactions (Calvin cycle) in the stroma, CO2 is fixed onto RuBP by rubisco to form glycerate 3-phosphate (GP).
  • GP is reduced to glyceraldehyde 3-phosphate (GALP) using ATP and reduced NADP.
  • Five out of every six GALP molecules regenerate RuBP, and the rest make glucose, sucrose, starch, cellulose, amino acids and lipids.

Part 2 of 7Ecosystems and the environment

Trophic level
  • Trophic level the feeding level of an organism in a food chain.
Energy transfer
  • Energy transfer it is inefficient because energy is lost in respiration, in waste and in uneaten parts.
Succession
  • Succession the predictable change in a community from pioneer species to a climax community, which has greater biodiversity than early stages.
Population estimates
  • Population estimates population = mean per quadrat × , and percentage cover = squares containing the species ÷ total squares × 100.
    • (total area ÷ quadrat area)
Abiotic measurements
  • Abiotic measurements light in lux, temperature in °C and soil pH with a probe.

Part 3 of 7Climate change

1Enhanced greenhouse effect
Enhanced greenhouse effect increase in the greenhouse effect caused by human activity raising the concentration of greenhouse gases (CO2, CH4),
  • so more infrared radiation is trapped.
  • Atmospheric CO2 rose from about 280 ppm pre-industrial to over 420 ppm.
2Calculations
  • Calculations percentage change = (new − original) ÷ original × 100, and Q10 = rate at (T + 10) °C ÷ rate at T °C, usually 2–3 for enzyme reactions.
EvidenceMeasuresLimitation
Temperature recordsDirect readings since about 1850Short span and fewer early records
Tree ringsRing width reflects growing conditionsOther factors, such as nutrients, affect width
Pollen in peatPast plant speciesPollen can be blown in
Ice coresCO2 in bubbles, temperature from isotopesOnly polar locations
1
Carbon cycle
  • Carbon cycle combustion of fossil fuels releases carbon stored for millions of years: CH4 + 2O2 → CO2 + 2H2O.
  • Mature forests are close to carbon neutral, whereas growing forests are net sinks.
  • Models that extrapolate data predict future temperatures but have uncertainty.
  • Rate of change from a graph change in y ÷ change in x, for example ppm per year.
2
Temperature and development practicals
  • Temperature and development practicals other variables are kept constant and extremes of temperature avoided.

Part 4 of 7Microbiology and immunity

Bacterial growth
  • Bacterial growth bacteria divide by binary fission,
    • so with a generation time of 20 minutes, 50 cells after 3 hours (9 generations) become 50 × 29 = 25 600 cells.
Aseptic technique
  • Aseptic technique flame loops and bottle necks, work near a Bunsen flame, tape (not seal) plates and incubate at 25 °C, not 37 °C, to avoid growing human pathogens.
Tuberculosis
  • Tuberculosis caused by Mycobacterium tuberculosis, spread by droplets, with bacteria surviving inside macrophages and forming tubercles.
T killer cells
  • T killer cells they bind complementary antigen on infected body cells and destroy them, for example with perforin that makes pores in the membrane.
B cells
  • B cells they form plasma cells that secrete antibodies, and memory cells.

Part 5 of 7Forensics

Forensics
Succession on a corpse
  • Succession on a corpse blowflies arrive first, then flesh flies, then beetles feeding on drier tissue, then mites and soil organisms.
  • The community present indicates the stage reached and so the time since death.
DNA profiling
  • DNA profiling it relies on PCR amplification and gel electrophoresis.
Evolutionary relationships
  • Evolutionary relationships they are inferred from DNA and protein sequence similarity using bioinformatics.
  • Bioinformatics is the use of computer software and databases to store and analyse biological data.

Part 6 of 7More from the specification

More from the specification
Photosynthetic Pigments and Chromatography
  • Leaf pigments are separated by chromatography using an organic solvent inside a chromatography chamber.
    • (chlorophyll a, chlorophyll b, carotene, xanthophyll)
Light-Dependent Reactions and Photosystems
  • In the thylakoid membranes of the chloroplast, light energy is absorbed by accessory pigments in photosystem II and I to excite electrons.
Photolysis of Water
  • Light-driven splitting of water in photosystem II generates hydrogen ions (protons), electrons,
  • and waste oxygen gas.
    • (2H₂O → 4H⁺ + 4e⁻ + O₂)
Carbon Dioxide Fixation and Light Intensity
  • Carbon dioxide is fixed into carbohydrates in the Calvin cycle;
  • increasing light intensity increases the rate of glucose produced until another factor becomes limiting.
Photosynthetic Pigments and Chromatography
  • Leaf pigments are separated by chromatography using an organic solvent inside a chromatography chamber.
    • (chlorophyll a, chlorophyll b, carotene, xanthophyll)
Light-Dependent Reactions and Photosystems
  • In the thylakoid membranes of the chloroplast, light energy is absorbed by accessory pigments in photosystem II and I to excite electrons.
Photolysis of Water
  • Light-driven splitting of water in photosystem II generates hydrogen ions (protons), electrons,
  • and waste oxygen gas.
    • (2H₂O → 4H⁺ + 4e⁻ + O₂)
Carbon Dioxide Fixation and Light Intensity
  • Carbon dioxide is fixed into carbohydrates in the Calvin cycle;
  • increasing light intensity increases the rate of glucose produced until another factor becomes limiting.
Light intensity
  • The amount of light reaching a plant.
Hydrogen
  • A flammable gas that burns with a squeaky pop.
Solvent
  • The liquid that a solute dissolves in.
Chloroplast
  • Organelle where photosynthesis takes place.
Chromatography
  • A technique to separate substances in a mixture.
Absorption
  • Movement of digested molecules into the blood or lymph.
Photophosphorylation
  • Production of ATP using light energy.

Part 7 of 7More on this topic

  • Specific immunity
    • B lymphocytes produce antibodies that bind a matching antigen, and T lymphocytes coordinate responses or destroy infected cells.

Key terms

TermMeaning
GALPGlyceraldehyde 3-phosphate, a 3-carbon sugar phosphate made in the Calvin cycle.
Trophic levelFeeding level of an organism in a food chain.
Enhanced greenhouse effectIncrease in the greenhouse effect due to human activities raising greenhouse gas concentrations.
T killer cellLymphocyte that destroys infected body cells displaying a specific antigen.
BioinformaticsUse of computers and databases to store and analyse biological data.

Avoid these mistakes

1
Saying the Calvin cycle needs no ATP — it uses ATP and reduced NADP.
2
Confusing GP and GALP — GP is formed first and is reduced to GALP.
3
Describing the greenhouse effect as the ozone hole — it is trapping of infrared radiation by greenhouse gases.
4
Forgetting to subtract the original value in a percentage change calculation.

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