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Lesson 2

Lesson 2: Recombinant DNA, Gene Cloning, PCR and Genetic Engineering

Lesson parts

2.1 Modern techniques of molecular technologyModern techniques of molecular technology2.1

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2.2 Nucleic acid hybridisationNucleic acid hybridisation2.2

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2.3 Restriction endonucleasesRestriction endonucleases2.3

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2.4 Cloning a gene in a plasmidCloning a gene in a plasmid2.4

2.5 Reverse transcriptase and cDNAReverse transcriptase and cDNA2.5

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2.6 Polymerase chain reaction (PCR)Polymerase chain reaction (PCR)2.6

2.7 Recombinant DNA and its applicationsRecombinant DNA and its applications2.7

2.8 Worked example 10 — design a cloning experimentWorked example 10 — design a cloning experiment2.8

2.9 Worked example 11 — hybridisation questionWorked example 11 — hybridisation question2.9

2.10 The Human Genome ProjectThe Human Genome Project2.10

2.11 Worked example 12 — why compare genomes?Worked example 12 — why compare genomes?2.11

2.12 Key termsKey terms2.12

2.13 Final recapFinal recap2.13