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Quiz

Lesson 2: Recombinant DNA, Gene Cloning, PCR and Genetic Engineering — Picture questions (exam style)

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  • Questions count: 300
  • Passing grade: 60%
Start Quiz
1.
A linear DNA of 3000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A linear DNA of 3000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 1, fragments with both = 3
double digest in lane 1, fragments with both = 4
double digest in lane 2, fragments with both = 4
double digest in lane 3, fragments with both = 5
2.
The graph shows the temperature profile of two PCR cycles, starting with 10 DNA molecule(s). Which option is correct?
The graph shows the temperature profile of two PCR cycles, starting with 10 DNA molecule(s). Which option is correct?
step X: extension by Taq polymerase, copies after 4 cycles = 160, time for 4 cycles = 9.33 min
step X: extension by Taq polymerase, copies after 4 cycles = 160, time for 4 cycles = 18.7 min
step X: primer annealing, copies after 4 cycles = 26, time for 4 cycles = 9.33 min
step X: extension by Taq polymerase, copies after 4 cycles = 26, time for 4 cycles = 9.33 min
3.
A linear DNA of 3000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A linear DNA of 3000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 2, fragments with both = 2
double digest in lane 1, fragments with both = 4
double digest in lane 1, fragments with both = 2
double digest in lane 2, fragments with both = 3
4.
The graph shows the temperature profile of two PCR cycles, starting with 5 DNA molecule(s). Which option is correct?
The graph shows the temperature profile of two PCR cycles, starting with 5 DNA molecule(s). Which option is correct?
step X: extension by Taq polymerase, copies after 4 cycles = 40, time for 4 cycles = 9.67 min
step X: extension by Taq polymerase, copies after 4 cycles = 40, time for 4 cycles = 13.7 min
step X: primer annealing, copies after 4 cycles = 40, time for 4 cycles = 9.67 min
step X: extension by Taq polymerase, copies after 4 cycles = 80, time for 4 cycles = 9.67 min
5.
A circular plasmid of 4000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A circular plasmid of 4000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 2, fragments with both = 3
double digest in lane 1, fragments with both = 2
double digest in lane 2, fragments with both = 2
double digest in lane 3, fragments with both = 3
6.
The graph shows the temperature profile of two PCR cycles, starting with 1 DNA molecule(s). Which option is correct?
The graph shows the temperature profile of two PCR cycles, starting with 1 DNA molecule(s). Which option is correct?
step X: primer annealing, copies after 9 cycles = 18, time for 9 cycles = 28.5 min
step X: extension by Taq polymerase, copies after 9 cycles = 256, time for 9 cycles = 28.5 min
step X: denaturation, copies after 9 cycles = 512, time for 9 cycles = 39 min
step X: primer annealing, copies after 9 cycles = 512, time for 9 cycles = 19.5 min
7.
A circular plasmid of 3000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A circular plasmid of 3000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 1, fragments with both = 4
double digest in lane 2, fragments with both = 3
double digest in lane 2, fragments with both = 4
double digest in lane 3, fragments with both = 3
8.
The graph shows the temperature profile of two PCR cycles, starting with 3 DNA molecule(s). Which option is correct?
The graph shows the temperature profile of two PCR cycles, starting with 3 DNA molecule(s). Which option is correct?
step X: extension by Taq polymerase, copies after 6 cycles = 67, time for 6 cycles = 36 min
step X: denaturation, copies after 6 cycles = 36, time for 6 cycles = 3 min
step X: denaturation, copies after 6 cycles = 67, time for 6 cycles = 3 min
step X: primer annealing, copies after 6 cycles = 192, time for 6 cycles = 18 min
9.
A linear DNA of 8000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A linear DNA of 8000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 3, fragments with both = 4
double digest in lane 1, fragments with both = 2
double digest in lane 1, fragments with both = 4
double digest in lane 3, fragments with both = 3
10.
The graph shows the temperature profile of two PCR cycles, starting with 5 DNA molecule(s). Which option is correct?
The graph shows the temperature profile of two PCR cycles, starting with 5 DNA molecule(s). Which option is correct?
step X: extension by Taq polymerase, copies after 12 cycles = 20480, time for 12 cycles = 32 min
step X: extension by Taq polymerase, copies after 12 cycles = 4101, time for 12 cycles = 32 min
step X: denaturation, copies after 12 cycles = 20480, time for 12 cycles = 32 min
step X: denaturation, copies after 12 cycles = 120, time for 12 cycles = 32 min
11.
A circular plasmid of 3000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A circular plasmid of 3000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 1, fragments with both = 1
double digest in lane 3, fragments with both = 3
double digest in lane 1, fragments with both = 2
double digest in lane 1, fragments with both = 3
12.
The graph shows the temperature profile of two PCR cycles, starting with 2 DNA molecule(s). Which option is correct?
The graph shows the temperature profile of two PCR cycles, starting with 2 DNA molecule(s). Which option is correct?
step X: extension by Taq polymerase, copies after 6 cycles = 64, time for 6 cycles = 30 min
step X: primer annealing, copies after 6 cycles = 64, time for 6 cycles = 2.5 min
step X: extension by Taq polymerase, copies after 6 cycles = 128, time for 6 cycles = 15 min
step X: primer annealing, copies after 6 cycles = 128, time for 6 cycles = 2.5 min
13.
A linear DNA of 5000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A linear DNA of 5000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 1, fragments with both = 3
double digest in lane 3, fragments with both = 3
double digest in lane 2, fragments with both = 4
double digest in lane 3, fragments with both = 4
14.
The graph shows the temperature profile of two PCR cycles, starting with 2 DNA molecule(s). Which option is correct?
The graph shows the temperature profile of two PCR cycles, starting with 2 DNA molecule(s). Which option is correct?
step X: primer annealing, copies after 8 cycles = 512, time for 8 cycles = 23.3 min
step X: primer annealing, copies after 8 cycles = 32, time for 8 cycles = 2.92 min
step X: extension by Taq polymerase, copies after 8 cycles = 256, time for 8 cycles = 46.7 min
step X: denaturation, copies after 8 cycles = 258, time for 8 cycles = 46.7 min
15.
A linear DNA of 5000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A linear DNA of 5000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 1, fragments with both = 6
double digest in lane 3, fragments with both = 4
double digest in lane 2, fragments with both = 5
double digest in lane 3, fragments with both = 6
16.
The graph shows the temperature profile of two PCR cycles, starting with 2 DNA molecule(s). Which option is correct?
The graph shows the temperature profile of two PCR cycles, starting with 2 DNA molecule(s). Which option is correct?
step X: denaturation, copies after 10 cycles = 1026, time for 10 cycles = 30 min
step X: denaturation, copies after 10 cycles = 1024, time for 10 cycles = 15 min
step X: denaturation, copies after 10 cycles = 2048, time for 10 cycles = 15 min
step X: extension by Taq polymerase, copies after 10 cycles = 1024, time for 10 cycles = 1.5 min
17.
A linear DNA of 3000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A linear DNA of 3000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 1, fragments with both = 5
double digest in lane 3, fragments with both = 4
double digest in lane 2, fragments with both = 3
double digest in lane 2, fragments with both = 5
18.
The graph shows the temperature profile of two PCR cycles, starting with 1 DNA molecule(s). Which option is correct?
The graph shows the temperature profile of two PCR cycles, starting with 1 DNA molecule(s). Which option is correct?
step X: extension by Taq polymerase, copies after 9 cycles = 513, time for 9 cycles = 2.08 min
step X: extension by Taq polymerase, copies after 9 cycles = 256, time for 9 cycles = 2.08 min
step X: primer annealing, copies after 9 cycles = 512, time for 9 cycles = 18.8 min
step X: extension by Taq polymerase, copies after 9 cycles = 256, time for 9 cycles = 37.5 min
19.
A circular plasmid of 5000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A circular plasmid of 5000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 3, fragments with both = 3
double digest in lane 1, fragments with both = 3
double digest in lane 2, fragments with both = 3
double digest in lane 2, fragments with both = 2
20.
The graph shows the temperature profile of two PCR cycles, starting with 3 DNA molecule(s). Which option is correct?
The graph shows the temperature profile of two PCR cycles, starting with 3 DNA molecule(s). Which option is correct?
step X: primer annealing, copies after 11 cycles = 2051, time for 11 cycles = 18.3 min
step X: primer annealing, copies after 11 cycles = 66, time for 11 cycles = 36.7 min
step X: denaturation, copies after 11 cycles = 66, time for 11 cycles = 29.3 min
step X: primer annealing, copies after 11 cycles = 6144, time for 11 cycles = 18.3 min
21.
A circular plasmid of 6000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A circular plasmid of 6000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 1, fragments with both = 3
double digest in lane 3, fragments with both = 3
double digest in lane 2, fragments with both = 2
double digest in lane 3, fragments with both = 4
22.
The graph shows the temperature profile of two PCR cycles, starting with 3 DNA molecule(s). Which option is correct?
The graph shows the temperature profile of two PCR cycles, starting with 3 DNA molecule(s). Which option is correct?
step X: primer annealing, copies after 6 cycles = 192, time for 6 cycles = 18 min
step X: extension by Taq polymerase, copies after 6 cycles = 67, time for 6 cycles = 12 min
step X: denaturation, copies after 6 cycles = 192, time for 6 cycles = 12 min
step X: denaturation, copies after 6 cycles = 192, time for 6 cycles = 2 min
23.
The graph shows the temperature profile of two PCR cycles, starting with 3 DNA molecule(s). Which option is correct?
The graph shows the temperature profile of two PCR cycles, starting with 3 DNA molecule(s). Which option is correct?
step X: primer annealing, copies after 12 cycles = 12288, time for 12 cycles = 38 min
step X: extension by Taq polymerase, copies after 12 cycles = 12288, time for 12 cycles = 2.17 min
step X: extension by Taq polymerase, copies after 12 cycles = 6144, time for 12 cycles = 38 min
step X: denaturation, copies after 12 cycles = 12288, time for 12 cycles = 26 min
24.
A linear DNA of 5000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A linear DNA of 5000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 3, fragments with both = 3
double digest in lane 1, fragments with both = 3
double digest in lane 1, fragments with both = 2
double digest in lane 3, fragments with both = 4
25.
The graph shows the temperature profile of two PCR cycles, starting with 10 DNA molecule(s). Which option is correct?
The graph shows the temperature profile of two PCR cycles, starting with 10 DNA molecule(s). Which option is correct?
step X: primer annealing, copies after 4 cycles = 160, time for 4 cycles = 10 min
step X: extension by Taq polymerase, copies after 4 cycles = 80, time for 4 cycles = 2.5 min
step X: denaturation, copies after 4 cycles = 160, time for 4 cycles = 14 min
step X: primer annealing, copies after 4 cycles = 26, time for 4 cycles = 14 min
26.
A circular plasmid of 6000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A circular plasmid of 6000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 3, fragments with both = 3
double digest in lane 3, fragments with both = 5
double digest in lane 2, fragments with both = 4
double digest in lane 1, fragments with both = 4
27.
The graph shows the temperature profile of two PCR cycles, starting with 1 DNA molecule(s). Which option is correct?
The graph shows the temperature profile of two PCR cycles, starting with 1 DNA molecule(s). Which option is correct?
step X: extension by Taq polymerase, copies after 4 cycles = 17, time for 4 cycles = 8 min
step X: denaturation, copies after 4 cycles = 16, time for 4 cycles = 12 min
step X: denaturation, copies after 4 cycles = 17, time for 4 cycles = 8 min
step X: primer annealing, copies after 4 cycles = 16, time for 4 cycles = 8 min
28.
The graph shows the temperature profile of two PCR cycles, starting with 3 DNA molecule(s). Which option is correct?
The graph shows the temperature profile of two PCR cycles, starting with 3 DNA molecule(s). Which option is correct?
step X: denaturation, copies after 12 cycles = 12288, time for 12 cycles = 18 min
step X: extension by Taq polymerase, copies after 12 cycles = 12288, time for 12 cycles = 18 min
step X: primer annealing, copies after 12 cycles = 4099, time for 12 cycles = 18 min
step X: primer annealing, copies after 12 cycles = 12288, time for 12 cycles = 18 min
29.
A linear DNA of 3000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A linear DNA of 3000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 1, fragments with both = 4
double digest in lane 1, fragments with both = 2
double digest in lane 3, fragments with both = 4
double digest in lane 1, fragments with both = 3
30.
The graph shows the temperature profile of two PCR cycles, starting with 1 DNA molecule(s). Which option is correct?
The graph shows the temperature profile of two PCR cycles, starting with 1 DNA molecule(s). Which option is correct?
step X: primer annealing, copies after 10 cycles = 1024, time for 10 cycles = 20 min
step X: denaturation, copies after 10 cycles = 20, time for 10 cycles = 2 min
step X: denaturation, copies after 10 cycles = 512, time for 10 cycles = 30 min
step X: extension by Taq polymerase, copies after 10 cycles = 1024, time for 10 cycles = 20 min
31.
A circular plasmid of 6000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A circular plasmid of 6000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 2, fragments with both = 3
double digest in lane 2, fragments with both = 4
double digest in lane 1, fragments with both = 3
double digest in lane 3, fragments with both = 3
32.
The graph shows the temperature profile of two PCR cycles, starting with 3 DNA molecule(s). Which option is correct?
The graph shows the temperature profile of two PCR cycles, starting with 3 DNA molecule(s). Which option is correct?
step X: denaturation, copies after 7 cycles = 42, time for 7 cycles = 15.2 min
step X: primer annealing, copies after 7 cycles = 192, time for 7 cycles = 15.2 min
step X: denaturation, copies after 7 cycles = 192, time for 7 cycles = 2.17 min
step X: extension by Taq polymerase, copies after 7 cycles = 384, time for 7 cycles = 15.2 min
33.
The graph shows the temperature profile of two PCR cycles, starting with 5 DNA molecule(s). Which option is correct?
The graph shows the temperature profile of two PCR cycles, starting with 5 DNA molecule(s). Which option is correct?
step X: extension by Taq polymerase, copies after 10 cycles = 2560, time for 10 cycles = 37.5 min
step X: primer annealing, copies after 10 cycles = 100, time for 10 cycles = 37.5 min
step X: extension by Taq polymerase, copies after 10 cycles = 5120, time for 10 cycles = 27.5 min
step X: primer annealing, copies after 10 cycles = 5120, time for 10 cycles = 27.5 min
34.
A circular plasmid of 4000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A circular plasmid of 4000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 1, fragments with both = 2
double digest in lane 2, fragments with both = 3
double digest in lane 2, fragments with both = 1
double digest in lane 2, fragments with both = 2
35.
The graph shows the temperature profile of two PCR cycles, starting with 2 DNA molecule(s). Which option is correct?
The graph shows the temperature profile of two PCR cycles, starting with 2 DNA molecule(s). Which option is correct?
step X: extension by Taq polymerase, copies after 8 cycles = 256, time for 8 cycles = 33.3 min
step X: extension by Taq polymerase, copies after 8 cycles = 512, time for 8 cycles = 16.7 min
step X: primer annealing, copies after 8 cycles = 32, time for 8 cycles = 24.7 min
step X: primer annealing, copies after 8 cycles = 32, time for 8 cycles = 16.7 min
36.
A circular plasmid of 4000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A circular plasmid of 4000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 1, fragments with both = 3
double digest in lane 1, fragments with both = 1
double digest in lane 1, fragments with both = 2
double digest in lane 3, fragments with both = 2
37.
The graph shows the temperature profile of two PCR cycles, starting with 5 DNA molecule(s). Which option is correct?
The graph shows the temperature profile of two PCR cycles, starting with 5 DNA molecule(s). Which option is correct?
step X: extension by Taq polymerase, copies after 10 cycles = 5120, time for 10 cycles = 20 min
step X: primer annealing, copies after 10 cycles = 5120, time for 10 cycles = 20 min
step X: extension by Taq polymerase, copies after 10 cycles = 5120, time for 10 cycles = 30 min
step X: primer annealing, copies after 10 cycles = 2560, time for 10 cycles = 2 min
38.
A linear DNA of 4000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A linear DNA of 4000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 1, fragments with both = 5
double digest in lane 3, fragments with both = 5
double digest in lane 3, fragments with both = 6
double digest in lane 1, fragments with both = 4
39.
The graph shows the temperature profile of two PCR cycles, starting with 2 DNA molecule(s). Which option is correct?
The graph shows the temperature profile of two PCR cycles, starting with 2 DNA molecule(s). Which option is correct?
step X: denaturation, copies after 10 cycles = 1024, time for 10 cycles = 25 min
step X: primer annealing, copies after 10 cycles = 2048, time for 10 cycles = 25 min
step X: denaturation, copies after 10 cycles = 1024, time for 10 cycles = 50 min
step X: denaturation, copies after 10 cycles = 1026, time for 10 cycles = 50 min
40.
A circular plasmid of 6000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A circular plasmid of 6000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 1, fragments with both = 4
double digest in lane 2, fragments with both = 3
double digest in lane 3, fragments with both = 4
double digest in lane 1, fragments with both = 2
41.
The graph shows the temperature profile of two PCR cycles, starting with 1 DNA molecule(s). Which option is correct?
The graph shows the temperature profile of two PCR cycles, starting with 1 DNA molecule(s). Which option is correct?
step X: denaturation, copies after 4 cycles = 17, time for 4 cycles = 2.42 min
step X: denaturation, copies after 4 cycles = 17, time for 4 cycles = 19.3 min
step X: primer annealing, copies after 4 cycles = 16, time for 4 cycles = 9.67 min
step X: extension by Taq polymerase, copies after 4 cycles = 16, time for 4 cycles = 9.67 min
42.
The graph shows the temperature profile of two PCR cycles, starting with 10 DNA molecule(s). Which option is correct?
The graph shows the temperature profile of two PCR cycles, starting with 10 DNA molecule(s). Which option is correct?
step X: primer annealing, copies after 6 cycles = 640, time for 6 cycles = 1.33 min
step X: extension by Taq polymerase, copies after 6 cycles = 640, time for 6 cycles = 8 min
step X: primer annealing, copies after 6 cycles = 74, time for 6 cycles = 14 min
step X: denaturation, copies after 6 cycles = 640, time for 6 cycles = 8 min
43.
A linear DNA of 8000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A linear DNA of 8000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 3, fragments with both = 5
double digest in lane 1, fragments with both = 6
double digest in lane 2, fragments with both = 5
double digest in lane 1, fragments with both = 4
44.
The graph shows the temperature profile of two PCR cycles, starting with 5 DNA molecule(s). Which option is correct?
The graph shows the temperature profile of two PCR cycles, starting with 5 DNA molecule(s). Which option is correct?
step X: primer annealing, copies after 8 cycles = 1280, time for 8 cycles = 37.3 min
step X: extension by Taq polymerase, copies after 8 cycles = 1280, time for 8 cycles = 18.7 min
step X: denaturation, copies after 8 cycles = 1280, time for 8 cycles = 2.33 min
step X: extension by Taq polymerase, copies after 8 cycles = 1280, time for 8 cycles = 26.7 min
45.
A linear DNA of 6000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A linear DNA of 6000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 3, fragments with both = 4
double digest in lane 2, fragments with both = 5
double digest in lane 1, fragments with both = 6
double digest in lane 3, fragments with both = 5
46.
The graph shows the temperature profile of two PCR cycles, starting with 10 DNA molecule(s). Which option is correct?
The graph shows the temperature profile of two PCR cycles, starting with 10 DNA molecule(s). Which option is correct?
step X: primer annealing, copies after 12 cycles = 40960, time for 12 cycles = 22 min
step X: extension by Taq polymerase, copies after 12 cycles = 40960, time for 12 cycles = 22 min
step X: extension by Taq polymerase, copies after 12 cycles = 4106, time for 12 cycles = 22 min
step X: denaturation, copies after 12 cycles = 4106, time for 12 cycles = 44 min
47.
A linear DNA of 6000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A linear DNA of 6000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 1, fragments with both = 5
double digest in lane 1, fragments with both = 4
double digest in lane 3, fragments with both = 4
double digest in lane 2, fragments with both = 3
48.
The graph shows the temperature profile of two PCR cycles, starting with 2 DNA molecule(s). Which option is correct?
The graph shows the temperature profile of two PCR cycles, starting with 2 DNA molecule(s). Which option is correct?
step X: primer annealing, copies after 6 cycles = 64, time for 6 cycles = 2.58 min
step X: extension by Taq polymerase, copies after 6 cycles = 24, time for 6 cycles = 15.5 min
step X: primer annealing, copies after 6 cycles = 128, time for 6 cycles = 15.5 min
step X: primer annealing, copies after 6 cycles = 128, time for 6 cycles = 2.58 min
49.
A circular plasmid of 8000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A circular plasmid of 8000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 2, fragments with both = 3
double digest in lane 2, fragments with both = 1
double digest in lane 1, fragments with both = 2
double digest in lane 3, fragments with both = 1
50.
The graph shows the temperature profile of two PCR cycles, starting with 2 DNA molecule(s). Which option is correct?
The graph shows the temperature profile of two PCR cycles, starting with 2 DNA molecule(s). Which option is correct?
step X: denaturation, copies after 9 cycles = 1024, time for 9 cycles = 27 min
step X: primer annealing, copies after 9 cycles = 1024, time for 9 cycles = 18 min
step X: denaturation, copies after 9 cycles = 1024, time for 9 cycles = 18 min
step X: extension by Taq polymerase, copies after 9 cycles = 512, time for 9 cycles = 18 min
51.
A circular plasmid of 6000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A circular plasmid of 6000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 2, fragments with both = 3
double digest in lane 1, fragments with both = 3
double digest in lane 3, fragments with both = 3
double digest in lane 3, fragments with both = 2
52.
A linear DNA of 8000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A linear DNA of 8000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 2, fragments with both = 4
double digest in lane 3, fragments with both = 4
double digest in lane 1, fragments with both = 5
double digest in lane 3, fragments with both = 6
53.
The graph shows the temperature profile of two PCR cycles, starting with 5 DNA molecule(s). Which option is correct?
The graph shows the temperature profile of two PCR cycles, starting with 5 DNA molecule(s). Which option is correct?
step X: denaturation, copies after 12 cycles = 120, time for 12 cycles = 27 min
step X: primer annealing, copies after 12 cycles = 20480, time for 12 cycles = 54 min
step X: primer annealing, copies after 12 cycles = 10240, time for 12 cycles = 27 min
step X: denaturation, copies after 12 cycles = 20480, time for 12 cycles = 27 min
54.
A linear DNA of 5000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A linear DNA of 5000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 2, fragments with both = 3
double digest in lane 3, fragments with both = 4
double digest in lane 3, fragments with both = 5
double digest in lane 2, fragments with both = 4
55.
The graph shows the temperature profile of two PCR cycles, starting with 3 DNA molecule(s). Which option is correct?
The graph shows the temperature profile of two PCR cycles, starting with 3 DNA molecule(s). Which option is correct?
step X: primer annealing, copies after 7 cycles = 384, time for 7 cycles = 12.8 min
step X: extension by Taq polymerase, copies after 7 cycles = 42, time for 7 cycles = 25.7 min
step X: denaturation, copies after 7 cycles = 42, time for 7 cycles = 1.83 min
step X: primer annealing, copies after 7 cycles = 384, time for 7 cycles = 25.7 min
56.
A circular plasmid of 8000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A circular plasmid of 8000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 2, fragments with both = 4
double digest in lane 1, fragments with both = 5
double digest in lane 2, fragments with both = 3
double digest in lane 1, fragments with both = 4
57.
A linear DNA of 8000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A linear DNA of 8000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 3, fragments with both = 4
double digest in lane 2, fragments with both = 5
double digest in lane 2, fragments with both = 3
double digest in lane 3, fragments with both = 3
58.
The graph shows the temperature profile of two PCR cycles, starting with 1 DNA molecule(s). Which option is correct?
The graph shows the temperature profile of two PCR cycles, starting with 1 DNA molecule(s). Which option is correct?
step X: extension by Taq polymerase, copies after 8 cycles = 256, time for 8 cycles = 20 min
step X: extension by Taq polymerase, copies after 8 cycles = 128, time for 8 cycles = 20 min
step X: extension by Taq polymerase, copies after 8 cycles = 16, time for 8 cycles = 40 min
step X: denaturation, copies after 8 cycles = 256, time for 8 cycles = 28 min
59.
A linear DNA of 6000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A linear DNA of 6000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 3, fragments with both = 4
double digest in lane 2, fragments with both = 5
double digest in lane 1, fragments with both = 4
double digest in lane 3, fragments with both = 3
60.
The graph shows the temperature profile of two PCR cycles, starting with 10 DNA molecule(s). Which option is correct?
The graph shows the temperature profile of two PCR cycles, starting with 10 DNA molecule(s). Which option is correct?
step X: denaturation, copies after 5 cycles = 320, time for 5 cycles = 14.2 min
step X: extension by Taq polymerase, copies after 5 cycles = 100, time for 5 cycles = 14.2 min
step X: primer annealing, copies after 5 cycles = 320, time for 5 cycles = 19.2 min
step X: extension by Taq polymerase, copies after 5 cycles = 100, time for 5 cycles = 28.3 min
61.
The graph shows the temperature profile of two PCR cycles, starting with 5 DNA molecule(s). Which option is correct?
The graph shows the temperature profile of two PCR cycles, starting with 5 DNA molecule(s). Which option is correct?
step X: extension by Taq polymerase, copies after 7 cycles = 640, time for 7 cycles = 14.6 min
step X: primer annealing, copies after 7 cycles = 640, time for 7 cycles = 14.6 min
step X: primer annealing, copies after 7 cycles = 133, time for 7 cycles = 29.2 min
step X: extension by Taq polymerase, copies after 7 cycles = 320, time for 7 cycles = 29.2 min
62.
A linear DNA of 3000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A linear DNA of 3000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 2, fragments with both = 5
double digest in lane 1, fragments with both = 4
double digest in lane 3, fragments with both = 4
double digest in lane 2, fragments with both = 3
63.
The graph shows the temperature profile of two PCR cycles, starting with 2 DNA molecule(s). Which option is correct?
The graph shows the temperature profile of two PCR cycles, starting with 2 DNA molecule(s). Which option is correct?
step X: extension by Taq polymerase, copies after 11 cycles = 44, time for 11 cycles = 36.7 min
step X: primer annealing, copies after 11 cycles = 4096, time for 11 cycles = 25.7 min
step X: extension by Taq polymerase, copies after 11 cycles = 4096, time for 11 cycles = 36.7 min
step X: extension by Taq polymerase, copies after 11 cycles = 44, time for 11 cycles = 2.33 min
64.
A linear DNA of 6000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A linear DNA of 6000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 1, fragments with both = 5
double digest in lane 1, fragments with both = 4
double digest in lane 2, fragments with both = 4
double digest in lane 3, fragments with both = 3
65.
A linear DNA of 5000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A linear DNA of 5000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 3, fragments with both = 4
double digest in lane 1, fragments with both = 5
double digest in lane 2, fragments with both = 4
double digest in lane 2, fragments with both = 5
66.
The graph shows the temperature profile of two PCR cycles, starting with 1 DNA molecule(s). Which option is correct?
The graph shows the temperature profile of two PCR cycles, starting with 1 DNA molecule(s). Which option is correct?
step X: extension by Taq polymerase, copies after 9 cycles = 512, time for 9 cycles = 15 min
step X: primer annealing, copies after 9 cycles = 18, time for 9 cycles = 1.67 min
step X: denaturation, copies after 9 cycles = 512, time for 9 cycles = 15 min
step X: denaturation, copies after 9 cycles = 513, time for 9 cycles = 15 min
67.
A linear DNA of 5000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A linear DNA of 5000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 3, fragments with both = 3
double digest in lane 1, fragments with both = 5
double digest in lane 2, fragments with both = 5
double digest in lane 1, fragments with both = 4
68.
The graph shows the temperature profile of two PCR cycles, starting with 2 DNA molecule(s). Which option is correct?
The graph shows the temperature profile of two PCR cycles, starting with 2 DNA molecule(s). Which option is correct?
step X: primer annealing, copies after 4 cycles = 16, time for 4 cycles = 16 min
step X: extension by Taq polymerase, copies after 4 cycles = 18, time for 4 cycles = 16 min
step X: denaturation, copies after 4 cycles = 32, time for 4 cycles = 8 min
step X: denaturation, copies after 4 cycles = 16, time for 4 cycles = 8 min
69.
A linear DNA of 5000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A linear DNA of 5000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 3, fragments with both = 5
double digest in lane 1, fragments with both = 5
double digest in lane 2, fragments with both = 4
double digest in lane 2, fragments with both = 5
70.
The graph shows the temperature profile of two PCR cycles, starting with 1 DNA molecule(s). Which option is correct?
The graph shows the temperature profile of two PCR cycles, starting with 1 DNA molecule(s). Which option is correct?
step X: primer annealing, copies after 7 cycles = 128, time for 7 cycles = 12.8 min
step X: extension by Taq polymerase, copies after 7 cycles = 64, time for 7 cycles = 1.83 min
step X: denaturation, copies after 7 cycles = 64, time for 7 cycles = 25.7 min
step X: extension by Taq polymerase, copies after 7 cycles = 128, time for 7 cycles = 12.8 min
71.
A circular plasmid of 8000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A circular plasmid of 8000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 3, fragments with both = 3
double digest in lane 3, fragments with both = 2
double digest in lane 1, fragments with both = 4
double digest in lane 2, fragments with both = 2
72.
A linear DNA of 8000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A linear DNA of 8000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 1, fragments with both = 4
double digest in lane 3, fragments with both = 4
double digest in lane 2, fragments with both = 3
double digest in lane 2, fragments with both = 4
73.
The graph shows the temperature profile of two PCR cycles, starting with 2 DNA molecule(s). Which option is correct?
The graph shows the temperature profile of two PCR cycles, starting with 2 DNA molecule(s). Which option is correct?
step X: primer annealing, copies after 10 cycles = 40, time for 10 cycles = 1.92 min
step X: denaturation, copies after 10 cycles = 1024, time for 10 cycles = 19.2 min
step X: primer annealing, copies after 10 cycles = 2048, time for 10 cycles = 1.92 min
step X: primer annealing, copies after 10 cycles = 2048, time for 10 cycles = 19.2 min
74.
A linear DNA of 4000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A linear DNA of 4000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 3, fragments with both = 4
double digest in lane 2, fragments with both = 4
double digest in lane 3, fragments with both = 5
double digest in lane 1, fragments with both = 5
75.
A linear DNA of 5000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A linear DNA of 5000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 3, fragments with both = 4
double digest in lane 2, fragments with both = 4
double digest in lane 1, fragments with both = 6
double digest in lane 3, fragments with both = 5
76.
The graph shows the temperature profile of two PCR cycles, starting with 3 DNA molecule(s). Which option is correct?
The graph shows the temperature profile of two PCR cycles, starting with 3 DNA molecule(s). Which option is correct?
step X: extension by Taq polymerase, copies after 6 cycles = 192, time for 6 cycles = 2.58 min
step X: extension by Taq polymerase, copies after 6 cycles = 192, time for 6 cycles = 15.5 min
step X: primer annealing, copies after 6 cycles = 67, time for 6 cycles = 15.5 min
step X: primer annealing, copies after 6 cycles = 67, time for 6 cycles = 31 min
77.
The graph shows the temperature profile of two PCR cycles, starting with 1 DNA molecule(s). Which option is correct?
The graph shows the temperature profile of two PCR cycles, starting with 1 DNA molecule(s). Which option is correct?
step X: denaturation, copies after 10 cycles = 1024, time for 10 cycles = 19.2 min
step X: primer annealing, copies after 10 cycles = 1025, time for 10 cycles = 29.2 min
step X: extension by Taq polymerase, copies after 10 cycles = 1024, time for 10 cycles = 19.2 min
step X: primer annealing, copies after 10 cycles = 1025, time for 10 cycles = 1.92 min
78.
The graph shows the temperature profile of two PCR cycles, starting with 1 DNA molecule(s). Which option is correct?
The graph shows the temperature profile of two PCR cycles, starting with 1 DNA molecule(s). Which option is correct?
step X: extension by Taq polymerase, copies after 6 cycles = 64, time for 6 cycles = 20 min
step X: extension by Taq polymerase, copies after 6 cycles = 64, time for 6 cycles = 1.67 min
step X: extension by Taq polymerase, copies after 6 cycles = 32, time for 6 cycles = 10 min
step X: extension by Taq polymerase, copies after 6 cycles = 64, time for 6 cycles = 10 min
79.
A linear DNA of 4000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A linear DNA of 4000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 3, fragments with both = 4
double digest in lane 3, fragments with both = 3
double digest in lane 1, fragments with both = 4
double digest in lane 1, fragments with both = 3
80.
A linear DNA of 8000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A linear DNA of 8000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 3, fragments with both = 3
double digest in lane 3, fragments with both = 4
double digest in lane 1, fragments with both = 4
double digest in lane 1, fragments with both = 3
81.
A circular plasmid of 4000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A circular plasmid of 4000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 2, fragments with both = 2
double digest in lane 3, fragments with both = 3
double digest in lane 1, fragments with both = 3
double digest in lane 1, fragments with both = 2
82.
A linear DNA of 5000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A linear DNA of 5000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 1, fragments with both = 5
double digest in lane 2, fragments with both = 5
double digest in lane 3, fragments with both = 4
double digest in lane 1, fragments with both = 4
83.
The graph shows the temperature profile of two PCR cycles, starting with 1 DNA molecule(s). Which option is correct?
The graph shows the temperature profile of two PCR cycles, starting with 1 DNA molecule(s). Which option is correct?
step X: denaturation, copies after 8 cycles = 256, time for 8 cycles = 2.5 min
step X: primer annealing, copies after 8 cycles = 257, time for 8 cycles = 20 min
step X: extension by Taq polymerase, copies after 8 cycles = 256, time for 8 cycles = 40 min
step X: extension by Taq polymerase, copies after 8 cycles = 256, time for 8 cycles = 20 min
84.
The graph shows the temperature profile of two PCR cycles, starting with 10 DNA molecule(s). Which option is correct?
The graph shows the temperature profile of two PCR cycles, starting with 10 DNA molecule(s). Which option is correct?
step X: primer annealing, copies after 5 cycles = 100, time for 5 cycles = 21.7 min
step X: primer annealing, copies after 5 cycles = 42, time for 5 cycles = 2.17 min
step X: denaturation, copies after 5 cycles = 320, time for 5 cycles = 10.8 min
step X: extension by Taq polymerase, copies after 5 cycles = 320, time for 5 cycles = 10.8 min
85.
A circular plasmid of 3000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A circular plasmid of 3000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 1, fragments with both = 4
double digest in lane 3, fragments with both = 5
double digest in lane 2, fragments with both = 4
double digest in lane 2, fragments with both = 5
86.
A linear DNA of 4000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A linear DNA of 4000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 2, fragments with both = 3
double digest in lane 2, fragments with both = 4
double digest in lane 3, fragments with both = 4
double digest in lane 1, fragments with both = 5
87.
A circular plasmid of 8000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A circular plasmid of 8000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 3, fragments with both = 4
double digest in lane 3, fragments with both = 2
double digest in lane 2, fragments with both = 2
double digest in lane 2, fragments with both = 3
88.
A linear DNA of 5000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A linear DNA of 5000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 1, fragments with both = 5
double digest in lane 2, fragments with both = 4
double digest in lane 2, fragments with both = 6
double digest in lane 1, fragments with both = 4
89.
A linear DNA of 5000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A linear DNA of 5000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 3, fragments with both = 5
double digest in lane 2, fragments with both = 4
double digest in lane 2, fragments with both = 6
double digest in lane 1, fragments with both = 6
90.
A circular plasmid of 4000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A circular plasmid of 4000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 2, fragments with both = 3
double digest in lane 3, fragments with both = 4
double digest in lane 2, fragments with both = 2
double digest in lane 3, fragments with both = 3
91.
A circular plasmid of 5000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A circular plasmid of 5000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 2, fragments with both = 3
double digest in lane 3, fragments with both = 4
double digest in lane 1, fragments with both = 4
double digest in lane 2, fragments with both = 4
92.
The graph shows the temperature profile of two PCR cycles, starting with 2 DNA molecule(s). Which option is correct?
The graph shows the temperature profile of two PCR cycles, starting with 2 DNA molecule(s). Which option is correct?
step X: denaturation, copies after 5 cycles = 32, time for 5 cycles = 29.2 min
step X: primer annealing, copies after 5 cycles = 34, time for 5 cycles = 19.6 min
step X: extension by Taq polymerase, copies after 5 cycles = 64, time for 5 cycles = 14.6 min
step X: denaturation, copies after 5 cycles = 32, time for 5 cycles = 14.6 min
93.
A linear DNA of 5000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A linear DNA of 5000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 2, fragments with both = 4
double digest in lane 1, fragments with both = 5
double digest in lane 3, fragments with both = 4
double digest in lane 3, fragments with both = 5
94.
A linear DNA of 6000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A linear DNA of 6000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 1, fragments with both = 4
double digest in lane 2, fragments with both = 4
double digest in lane 3, fragments with both = 4
double digest in lane 2, fragments with both = 5
95.
A linear DNA of 3000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A linear DNA of 3000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 1, fragments with both = 4
double digest in lane 3, fragments with both = 3
double digest in lane 2, fragments with both = 2
double digest in lane 1, fragments with both = 2
96.
The graph shows the temperature profile of two PCR cycles, starting with 1 DNA molecule(s). Which option is correct?
The graph shows the temperature profile of two PCR cycles, starting with 1 DNA molecule(s). Which option is correct?
step X: primer annealing, copies after 12 cycles = 2048, time for 12 cycles = 30 min
step X: extension by Taq polymerase, copies after 12 cycles = 4096, time for 12 cycles = 1.5 min
step X: denaturation, copies after 12 cycles = 4096, time for 12 cycles = 18 min
step X: primer annealing, copies after 12 cycles = 4096, time for 12 cycles = 30 min
97.
A circular plasmid of 6000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A circular plasmid of 6000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 1, fragments with both = 4
double digest in lane 3, fragments with both = 4
double digest in lane 1, fragments with both = 3
double digest in lane 1, fragments with both = 5
98.
A linear DNA of 3000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A linear DNA of 3000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 3, fragments with both = 3
double digest in lane 3, fragments with both = 4
double digest in lane 1, fragments with both = 4
double digest in lane 1, fragments with both = 2
99.
The graph shows the temperature profile of two PCR cycles, starting with 10 DNA molecule(s). Which option is correct?
The graph shows the temperature profile of two PCR cycles, starting with 10 DNA molecule(s). Which option is correct?
step X: denaturation, copies after 4 cycles = 80, time for 4 cycles = 2.33 min
step X: denaturation, copies after 4 cycles = 160, time for 4 cycles = 9.33 min
step X: primer annealing, copies after 4 cycles = 26, time for 4 cycles = 9.33 min
step X: denaturation, copies after 4 cycles = 160, time for 4 cycles = 2.33 min
100.
A linear DNA of 5000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A linear DNA of 5000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 2, fragments with both = 4
double digest in lane 1, fragments with both = 3
double digest in lane 3, fragments with both = 3
double digest in lane 3, fragments with both = 5
101.
The graph shows the temperature profile of two PCR cycles, starting with 5 DNA molecule(s). Which option is correct?
The graph shows the temperature profile of two PCR cycles, starting with 5 DNA molecule(s). Which option is correct?
step X: primer annealing, copies after 10 cycles = 100, time for 10 cycles = 43.3 min
step X: extension by Taq polymerase, copies after 10 cycles = 5120, time for 10 cycles = 21.7 min
step X: denaturation, copies after 10 cycles = 2560, time for 10 cycles = 21.7 min
step X: denaturation, copies after 10 cycles = 5120, time for 10 cycles = 21.7 min
102.
A linear DNA of 6000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A linear DNA of 6000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 3, fragments with both = 5
double digest in lane 2, fragments with both = 3
double digest in lane 2, fragments with both = 5
double digest in lane 2, fragments with both = 4
103.
The graph shows the temperature profile of two PCR cycles, starting with 3 DNA molecule(s). Which option is correct?
The graph shows the temperature profile of two PCR cycles, starting with 3 DNA molecule(s). Which option is correct?
step X: denaturation, copies after 4 cycles = 48, time for 4 cycles = 22.7 min
step X: denaturation, copies after 4 cycles = 24, time for 4 cycles = 15.3 min
step X: extension by Taq polymerase, copies after 4 cycles = 48, time for 4 cycles = 11.3 min
step X: denaturation, copies after 4 cycles = 48, time for 4 cycles = 2.83 min
104.
A linear DNA of 5000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A linear DNA of 5000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 3, fragments with both = 3
double digest in lane 3, fragments with both = 4
double digest in lane 1, fragments with both = 4
double digest in lane 2, fragments with both = 3
105.
A linear DNA of 4000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A linear DNA of 4000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 2, fragments with both = 4
double digest in lane 3, fragments with both = 3
double digest in lane 1, fragments with both = 4
double digest in lane 2, fragments with both = 5
106.
A circular plasmid of 8000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A circular plasmid of 8000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 1, fragments with both = 4
double digest in lane 3, fragments with both = 3
double digest in lane 2, fragments with both = 4
double digest in lane 1, fragments with both = 3
107.
A circular plasmid of 6000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A circular plasmid of 6000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 2, fragments with both = 3
double digest in lane 2, fragments with both = 2
double digest in lane 1, fragments with both = 4
double digest in lane 3, fragments with both = 3
108.
The graph shows the temperature profile of two PCR cycles, starting with 1 DNA molecule(s). Which option is correct?
The graph shows the temperature profile of two PCR cycles, starting with 1 DNA molecule(s). Which option is correct?
step X: primer annealing, copies after 10 cycles = 1025, time for 10 cycles = 53.3 min
step X: primer annealing, copies after 10 cycles = 20, time for 10 cycles = 2.67 min
step X: denaturation, copies after 10 cycles = 1024, time for 10 cycles = 26.7 min
step X: denaturation, copies after 10 cycles = 1024, time for 10 cycles = 2.67 min
109.
The graph shows the temperature profile of two PCR cycles, starting with 5 DNA molecule(s). Which option is correct?
The graph shows the temperature profile of two PCR cycles, starting with 5 DNA molecule(s). Which option is correct?
step X: primer annealing, copies after 6 cycles = 60, time for 6 cycles = 12.5 min
step X: primer annealing, copies after 6 cycles = 320, time for 6 cycles = 12.5 min
step X: denaturation, copies after 6 cycles = 320, time for 6 cycles = 25 min
step X: extension by Taq polymerase, copies after 6 cycles = 69, time for 6 cycles = 2.08 min
110.
A linear DNA of 6000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A linear DNA of 6000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 2, fragments with both = 4
double digest in lane 3, fragments with both = 4
double digest in lane 1, fragments with both = 3
double digest in lane 2, fragments with both = 3
111.
A circular plasmid of 4000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A circular plasmid of 4000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 2, fragments with both = 4
double digest in lane 3, fragments with both = 5
double digest in lane 2, fragments with both = 5
double digest in lane 3, fragments with both = 3
112.
A circular plasmid of 6000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A circular plasmid of 6000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 2, fragments with both = 3
double digest in lane 2, fragments with both = 5
double digest in lane 2, fragments with both = 4
double digest in lane 1, fragments with both = 3
113.
The graph shows the temperature profile of two PCR cycles, starting with 5 DNA molecule(s). Which option is correct?
The graph shows the temperature profile of two PCR cycles, starting with 5 DNA molecule(s). Which option is correct?
step X: primer annealing, copies after 8 cycles = 261, time for 8 cycles = 2.67 min
step X: extension by Taq polymerase, copies after 8 cycles = 80, time for 8 cycles = 21.3 min
step X: extension by Taq polymerase, copies after 8 cycles = 1280, time for 8 cycles = 21.3 min
step X: denaturation, copies after 8 cycles = 640, time for 8 cycles = 2.67 min
114.
A linear DNA of 3000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A linear DNA of 3000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 2, fragments with both = 3
double digest in lane 3, fragments with both = 4
double digest in lane 1, fragments with both = 3
double digest in lane 2, fragments with both = 4
115.
A circular plasmid of 8000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A circular plasmid of 8000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 2, fragments with both = 3
double digest in lane 3, fragments with both = 5
double digest in lane 2, fragments with both = 4
double digest in lane 3, fragments with both = 4
116.
A circular plasmid of 4000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A circular plasmid of 4000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 1, fragments with both = 5
double digest in lane 2, fragments with both = 3
double digest in lane 2, fragments with both = 4
double digest in lane 3, fragments with both = 3
117.
A linear DNA of 8000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A linear DNA of 8000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 3, fragments with both = 4
double digest in lane 2, fragments with both = 2
double digest in lane 2, fragments with both = 3
double digest in lane 2, fragments with both = 4
118.
A circular plasmid of 6000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A circular plasmid of 6000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 3, fragments with both = 2
double digest in lane 2, fragments with both = 2
double digest in lane 2, fragments with both = 3
double digest in lane 1, fragments with both = 3
119.
The graph shows the temperature profile of two PCR cycles, starting with 1 DNA molecule(s). Which option is correct?
The graph shows the temperature profile of two PCR cycles, starting with 1 DNA molecule(s). Which option is correct?
step X: denaturation, copies after 9 cycles = 18, time for 9 cycles = 31.5 min
step X: extension by Taq polymerase, copies after 9 cycles = 256, time for 9 cycles = 45 min
step X: primer annealing, copies after 9 cycles = 512, time for 9 cycles = 22.5 min
step X: denaturation, copies after 9 cycles = 513, time for 9 cycles = 31.5 min
120.
A linear DNA of 8000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A linear DNA of 8000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 2, fragments with both = 6
double digest in lane 3, fragments with both = 5
double digest in lane 3, fragments with both = 4
double digest in lane 1, fragments with both = 4
121.
The graph shows the temperature profile of two PCR cycles, starting with 1 DNA molecule(s). Which option is correct?
The graph shows the temperature profile of two PCR cycles, starting with 1 DNA molecule(s). Which option is correct?
step X: extension by Taq polymerase, copies after 4 cycles = 16, time for 4 cycles = 5.33 min
step X: denaturation, copies after 4 cycles = 16, time for 4 cycles = 5.33 min
step X: denaturation, copies after 4 cycles = 8, time for 4 cycles = 9.33 min
step X: denaturation, copies after 4 cycles = 17, time for 4 cycles = 10.7 min
122.
A linear DNA of 4000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A linear DNA of 4000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 2, fragments with both = 4
double digest in lane 1, fragments with both = 4
double digest in lane 3, fragments with both = 5
double digest in lane 1, fragments with both = 6
123.
A linear DNA of 8000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A linear DNA of 8000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 2, fragments with both = 6
double digest in lane 3, fragments with both = 5
double digest in lane 1, fragments with both = 6
double digest in lane 1, fragments with both = 4
124.
A linear DNA of 6000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A linear DNA of 6000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 1, fragments with both = 2
double digest in lane 3, fragments with both = 4
double digest in lane 3, fragments with both = 2
double digest in lane 2, fragments with both = 3
125.
The graph shows the temperature profile of two PCR cycles, starting with 1 DNA molecule(s). Which option is correct?
The graph shows the temperature profile of two PCR cycles, starting with 1 DNA molecule(s). Which option is correct?
step X: denaturation, copies after 4 cycles = 16, time for 4 cycles = 1.33 min
step X: denaturation, copies after 4 cycles = 16, time for 4 cycles = 5.33 min
step X: extension by Taq polymerase, copies after 4 cycles = 16, time for 4 cycles = 10.7 min
step X: primer annealing, copies after 4 cycles = 8, time for 4 cycles = 1.33 min
126.
A linear DNA of 8000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A linear DNA of 8000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 2, fragments with both = 3
double digest in lane 3, fragments with both = 3
double digest in lane 2, fragments with both = 5
double digest in lane 1, fragments with both = 4
127.
The graph shows the temperature profile of two PCR cycles, starting with 3 DNA molecule(s). Which option is correct?
The graph shows the temperature profile of two PCR cycles, starting with 3 DNA molecule(s). Which option is correct?
step X: extension by Taq polymerase, copies after 8 cycles = 259, time for 8 cycles = 2.33 min
step X: denaturation, copies after 8 cycles = 768, time for 8 cycles = 18.7 min
step X: denaturation, copies after 8 cycles = 384, time for 8 cycles = 37.3 min
step X: extension by Taq polymerase, copies after 8 cycles = 768, time for 8 cycles = 26.7 min
128.
The graph shows the temperature profile of two PCR cycles, starting with 10 DNA molecule(s). Which option is correct?
The graph shows the temperature profile of two PCR cycles, starting with 10 DNA molecule(s). Which option is correct?
step X: extension by Taq polymerase, copies after 6 cycles = 640, time for 6 cycles = 32 min
step X: primer annealing, copies after 6 cycles = 640, time for 6 cycles = 32 min
step X: denaturation, copies after 6 cycles = 320, time for 6 cycles = 22 min
step X: denaturation, copies after 6 cycles = 640, time for 6 cycles = 16 min
129.
A circular plasmid of 5000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A circular plasmid of 5000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 2, fragments with both = 4
double digest in lane 1, fragments with both = 2
double digest in lane 1, fragments with both = 4
double digest in lane 3, fragments with both = 3
130.
The graph shows the temperature profile of two PCR cycles, starting with 10 DNA molecule(s). Which option is correct?
The graph shows the temperature profile of two PCR cycles, starting with 10 DNA molecule(s). Which option is correct?
step X: primer annealing, copies after 4 cycles = 160, time for 4 cycles = 9.33 min
step X: extension by Taq polymerase, copies after 4 cycles = 80, time for 4 cycles = 18.7 min
step X: extension by Taq polymerase, copies after 4 cycles = 160, time for 4 cycles = 2.33 min
step X: primer annealing, copies after 4 cycles = 80, time for 4 cycles = 9.33 min
131.
A linear DNA of 5000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A linear DNA of 5000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 3, fragments with both = 6
double digest in lane 3, fragments with both = 4
double digest in lane 3, fragments with both = 5
double digest in lane 1, fragments with both = 4
132.
A circular plasmid of 3000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A circular plasmid of 3000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 1, fragments with both = 2
double digest in lane 3, fragments with both = 3
double digest in lane 3, fragments with both = 1
double digest in lane 3, fragments with both = 2
133.
A linear DNA of 4000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A linear DNA of 4000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 1, fragments with both = 4
double digest in lane 1, fragments with both = 6
double digest in lane 1, fragments with both = 5
double digest in lane 3, fragments with both = 5
134.
The graph shows the temperature profile of two PCR cycles, starting with 10 DNA molecule(s). Which option is correct?
The graph shows the temperature profile of two PCR cycles, starting with 10 DNA molecule(s). Which option is correct?
step X: extension by Taq polymerase, copies after 9 cycles = 180, time for 9 cycles = 1.5 min
step X: extension by Taq polymerase, copies after 9 cycles = 5120, time for 9 cycles = 13.5 min
step X: extension by Taq polymerase, copies after 9 cycles = 5120, time for 9 cycles = 1.5 min
step X: denaturation, copies after 9 cycles = 180, time for 9 cycles = 22.5 min
135.
A linear DNA of 6000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A linear DNA of 6000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 2, fragments with both = 4
double digest in lane 1, fragments with both = 4
double digest in lane 2, fragments with both = 5
double digest in lane 3, fragments with both = 3
136.
The graph shows the temperature profile of two PCR cycles, starting with 10 DNA molecule(s). Which option is correct?
The graph shows the temperature profile of two PCR cycles, starting with 10 DNA molecule(s). Which option is correct?
step X: denaturation, copies after 11 cycles = 2058, time for 11 cycles = 25.7 min
step X: primer annealing, copies after 11 cycles = 20480, time for 11 cycles = 25.7 min
step X: denaturation, copies after 11 cycles = 20480, time for 11 cycles = 25.7 min
step X: extension by Taq polymerase, copies after 11 cycles = 220, time for 11 cycles = 25.7 min
137.
A circular plasmid of 8000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A circular plasmid of 8000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 2, fragments with both = 4
double digest in lane 3, fragments with both = 4
double digest in lane 1, fragments with both = 5
double digest in lane 2, fragments with both = 5
138.
The graph shows the temperature profile of two PCR cycles, starting with 3 DNA molecule(s). Which option is correct?
The graph shows the temperature profile of two PCR cycles, starting with 3 DNA molecule(s). Which option is correct?
step X: denaturation, copies after 9 cycles = 1536, time for 9 cycles = 22.5 min
step X: primer annealing, copies after 9 cycles = 768, time for 9 cycles = 31.5 min
step X: denaturation, copies after 9 cycles = 1536, time for 9 cycles = 31.5 min
step X: extension by Taq polymerase, copies after 9 cycles = 768, time for 9 cycles = 2.5 min
139.
A linear DNA of 3000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A linear DNA of 3000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 2, fragments with both = 5
double digest in lane 3, fragments with both = 4
double digest in lane 2, fragments with both = 4
double digest in lane 3, fragments with both = 5
140.
A linear DNA of 5000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A linear DNA of 5000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 1, fragments with both = 4
double digest in lane 3, fragments with both = 5
double digest in lane 2, fragments with both = 4
double digest in lane 3, fragments with both = 4
141.
A circular plasmid of 3000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A circular plasmid of 3000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 2, fragments with both = 3
double digest in lane 1, fragments with both = 4
double digest in lane 3, fragments with both = 5
double digest in lane 1, fragments with both = 3
142.
A linear DNA of 3000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A linear DNA of 3000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 2, fragments with both = 5
double digest in lane 2, fragments with both = 4
double digest in lane 1, fragments with both = 3
double digest in lane 1, fragments with both = 5
143.
A circular plasmid of 6000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A circular plasmid of 6000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 2, fragments with both = 5
double digest in lane 1, fragments with both = 5
double digest in lane 3, fragments with both = 4
double digest in lane 1, fragments with both = 3
144.
A linear DNA of 4000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A linear DNA of 4000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 3, fragments with both = 3
double digest in lane 2, fragments with both = 5
double digest in lane 1, fragments with both = 3
double digest in lane 2, fragments with both = 4
145.
A circular plasmid of 5000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A circular plasmid of 5000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 3, fragments with both = 4
double digest in lane 1, fragments with both = 3
double digest in lane 3, fragments with both = 2
double digest in lane 2, fragments with both = 2
146.
The graph shows the temperature profile of two PCR cycles, starting with 3 DNA molecule(s). Which option is correct?
The graph shows the temperature profile of two PCR cycles, starting with 3 DNA molecule(s). Which option is correct?
step X: denaturation, copies after 11 cycles = 6144, time for 11 cycles = 34.8 min
step X: extension by Taq polymerase, copies after 11 cycles = 6144, time for 11 cycles = 69.7 min
step X: extension by Taq polymerase, copies after 11 cycles = 6144, time for 11 cycles = 34.8 min
step X: primer annealing, copies after 11 cycles = 6144, time for 11 cycles = 34.8 min
147.
A linear DNA of 5000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A linear DNA of 5000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 2, fragments with both = 5
double digest in lane 3, fragments with both = 4
double digest in lane 1, fragments with both = 4
double digest in lane 3, fragments with both = 5
148.
A circular plasmid of 6000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A circular plasmid of 6000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 2, fragments with both = 2
double digest in lane 3, fragments with both = 3
double digest in lane 2, fragments with both = 3
double digest in lane 2, fragments with both = 4
149.
The graph shows the temperature profile of two PCR cycles, starting with 2 DNA molecule(s). Which option is correct?
The graph shows the temperature profile of two PCR cycles, starting with 2 DNA molecule(s). Which option is correct?
step X: denaturation, copies after 7 cycles = 256, time for 7 cycles = 2.5 min
step X: primer annealing, copies after 7 cycles = 256, time for 7 cycles = 24.5 min
step X: primer annealing, copies after 7 cycles = 256, time for 7 cycles = 17.5 min
step X: extension by Taq polymerase, copies after 7 cycles = 256, time for 7 cycles = 35 min
150.
The graph shows the temperature profile of two PCR cycles, starting with 3 DNA molecule(s). Which option is correct?
The graph shows the temperature profile of two PCR cycles, starting with 3 DNA molecule(s). Which option is correct?
step X: denaturation, copies after 11 cycles = 2051, time for 11 cycles = 42.2 min
step X: extension by Taq polymerase, copies after 11 cycles = 2051, time for 11 cycles = 62.3 min
step X: extension by Taq polymerase, copies after 11 cycles = 6144, time for 11 cycles = 31.2 min
step X: primer annealing, copies after 11 cycles = 6144, time for 11 cycles = 2.83 min
151.
A linear DNA of 6000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A linear DNA of 6000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 3, fragments with both = 6
double digest in lane 1, fragments with both = 5
double digest in lane 1, fragments with both = 4
double digest in lane 3, fragments with both = 5
152.
The graph shows the temperature profile of two PCR cycles, starting with 2 DNA molecule(s). Which option is correct?
The graph shows the temperature profile of two PCR cycles, starting with 2 DNA molecule(s). Which option is correct?
step X: extension by Taq polymerase, copies after 9 cycles = 1024, time for 9 cycles = 16.5 min
step X: primer annealing, copies after 9 cycles = 1024, time for 9 cycles = 16.5 min
step X: primer annealing, copies after 9 cycles = 36, time for 9 cycles = 16.5 min
step X: denaturation, copies after 9 cycles = 1024, time for 9 cycles = 16.5 min
153.
A circular plasmid of 5000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A circular plasmid of 5000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 1, fragments with both = 3
double digest in lane 3, fragments with both = 4
double digest in lane 3, fragments with both = 5
double digest in lane 2, fragments with both = 4
154.
A linear DNA of 6000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A linear DNA of 6000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 2, fragments with both = 4
double digest in lane 3, fragments with both = 4
double digest in lane 2, fragments with both = 5
double digest in lane 2, fragments with both = 6
155.
A linear DNA of 4000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A linear DNA of 4000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 2, fragments with both = 4
double digest in lane 3, fragments with both = 6
double digest in lane 2, fragments with both = 5
double digest in lane 3, fragments with both = 5
156.
The graph shows the temperature profile of two PCR cycles, starting with 10 DNA molecule(s). Which option is correct?
The graph shows the temperature profile of two PCR cycles, starting with 10 DNA molecule(s). Which option is correct?
step X: denaturation, copies after 6 cycles = 640, time for 6 cycles = 14 min
step X: denaturation, copies after 6 cycles = 640, time for 6 cycles = 2.33 min
step X: extension by Taq polymerase, copies after 6 cycles = 640, time for 6 cycles = 20 min
step X: extension by Taq polymerase, copies after 6 cycles = 640, time for 6 cycles = 14 min
157.
A linear DNA of 3000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A linear DNA of 3000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 3, fragments with both = 2
double digest in lane 2, fragments with both = 2
double digest in lane 1, fragments with both = 2
double digest in lane 1, fragments with both = 3
158.
A linear DNA of 8000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A linear DNA of 8000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 2, fragments with both = 5
double digest in lane 3, fragments with both = 4
double digest in lane 3, fragments with both = 5
double digest in lane 2, fragments with both = 6
159.
The graph shows the temperature profile of two PCR cycles, starting with 2 DNA molecule(s). Which option is correct?
The graph shows the temperature profile of two PCR cycles, starting with 2 DNA molecule(s). Which option is correct?
step X: primer annealing, copies after 10 cycles = 1024, time for 10 cycles = 18.3 min
step X: extension by Taq polymerase, copies after 10 cycles = 2048, time for 10 cycles = 18.3 min
step X: primer annealing, copies after 10 cycles = 40, time for 10 cycles = 28.3 min
step X: extension by Taq polymerase, copies after 10 cycles = 1026, time for 10 cycles = 28.3 min
160.
A linear DNA of 5000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A linear DNA of 5000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 2, fragments with both = 2
double digest in lane 1, fragments with both = 3
double digest in lane 1, fragments with both = 4
double digest in lane 3, fragments with both = 3
161.
A linear DNA of 3000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A linear DNA of 3000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 3, fragments with both = 4
double digest in lane 2, fragments with both = 5
double digest in lane 2, fragments with both = 3
double digest in lane 3, fragments with both = 5
162.
The graph shows the temperature profile of two PCR cycles, starting with 2 DNA molecule(s). Which option is correct?
The graph shows the temperature profile of two PCR cycles, starting with 2 DNA molecule(s). Which option is correct?
step X: denaturation, copies after 9 cycles = 1024, time for 9 cycles = 21 min
step X: extension by Taq polymerase, copies after 9 cycles = 36, time for 9 cycles = 42 min
step X: denaturation, copies after 9 cycles = 512, time for 9 cycles = 42 min
step X: denaturation, copies after 9 cycles = 514, time for 9 cycles = 21 min
163.
A linear DNA of 3000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A linear DNA of 3000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 3, fragments with both = 5
double digest in lane 3, fragments with both = 4
double digest in lane 2, fragments with both = 5
double digest in lane 1, fragments with both = 5
164.
A linear DNA of 4000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A linear DNA of 4000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 3, fragments with both = 4
double digest in lane 1, fragments with both = 5
double digest in lane 2, fragments with both = 6
double digest in lane 1, fragments with both = 4
165.
The graph shows the temperature profile of two PCR cycles, starting with 1 DNA molecule(s). Which option is correct?
The graph shows the temperature profile of two PCR cycles, starting with 1 DNA molecule(s). Which option is correct?
step X: extension by Taq polymerase, copies after 5 cycles = 10, time for 5 cycles = 15.8 min
step X: primer annealing, copies after 5 cycles = 32, time for 5 cycles = 31.7 min
step X: primer annealing, copies after 5 cycles = 32, time for 5 cycles = 15.8 min
step X: denaturation, copies after 5 cycles = 32, time for 5 cycles = 3.17 min
166.
A circular plasmid of 6000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A circular plasmid of 6000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 1, fragments with both = 4
double digest in lane 3, fragments with both = 4
double digest in lane 3, fragments with both = 5
double digest in lane 1, fragments with both = 3
167.
A circular plasmid of 4000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A circular plasmid of 4000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 1, fragments with both = 4
double digest in lane 3, fragments with both = 4
double digest in lane 3, fragments with both = 3
double digest in lane 2, fragments with both = 4
168.
A linear DNA of 3000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A linear DNA of 3000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 2, fragments with both = 5
double digest in lane 1, fragments with both = 4
double digest in lane 1, fragments with both = 3
double digest in lane 2, fragments with both = 4
169.
A linear DNA of 6000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A linear DNA of 6000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 1, fragments with both = 5
double digest in lane 3, fragments with both = 4
double digest in lane 2, fragments with both = 3
double digest in lane 2, fragments with both = 4
170.
A circular plasmid of 4000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A circular plasmid of 4000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 1, fragments with both = 2
double digest in lane 2, fragments with both = 2
double digest in lane 3, fragments with both = 3
double digest in lane 1, fragments with both = 4
171.
A circular plasmid of 3000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A circular plasmid of 3000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 3, fragments with both = 4
double digest in lane 2, fragments with both = 4
double digest in lane 2, fragments with both = 2
double digest in lane 1, fragments with both = 3
172.
A linear DNA of 4000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A linear DNA of 4000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 1, fragments with both = 3
double digest in lane 3, fragments with both = 4
double digest in lane 2, fragments with both = 3
double digest in lane 2, fragments with both = 4
173.
The graph shows the temperature profile of two PCR cycles, starting with 10 DNA molecule(s). Which option is correct?
The graph shows the temperature profile of two PCR cycles, starting with 10 DNA molecule(s). Which option is correct?
step X: extension by Taq polymerase, copies after 4 cycles = 80, time for 4 cycles = 18 min
step X: extension by Taq polymerase, copies after 4 cycles = 160, time for 4 cycles = 9 min
step X: extension by Taq polymerase, copies after 4 cycles = 26, time for 4 cycles = 18 min
step X: primer annealing, copies after 4 cycles = 160, time for 4 cycles = 9 min
174.
The graph shows the temperature profile of two PCR cycles, starting with 2 DNA molecule(s). Which option is correct?
The graph shows the temperature profile of two PCR cycles, starting with 2 DNA molecule(s). Which option is correct?
step X: denaturation, copies after 10 cycles = 1026, time for 10 cycles = 2.17 min
step X: primer annealing, copies after 10 cycles = 2048, time for 10 cycles = 21.7 min
step X: extension by Taq polymerase, copies after 10 cycles = 2048, time for 10 cycles = 43.3 min
step X: primer annealing, copies after 10 cycles = 1024, time for 10 cycles = 21.7 min
175.
A circular plasmid of 5000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A circular plasmid of 5000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 2, fragments with both = 4
double digest in lane 1, fragments with both = 3
double digest in lane 2, fragments with both = 3
double digest in lane 1, fragments with both = 4
176.
The graph shows the temperature profile of two PCR cycles, starting with 1 DNA molecule(s). Which option is correct?
The graph shows the temperature profile of two PCR cycles, starting with 1 DNA molecule(s). Which option is correct?
step X: denaturation, copies after 12 cycles = 24, time for 12 cycles = 2.67 min
step X: extension by Taq polymerase, copies after 12 cycles = 4097, time for 12 cycles = 2.67 min
step X: primer annealing, copies after 12 cycles = 4096, time for 12 cycles = 32 min
step X: extension by Taq polymerase, copies after 12 cycles = 4096, time for 12 cycles = 32 min
177.
A circular plasmid of 4000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A circular plasmid of 4000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 1, fragments with both = 3
double digest in lane 1, fragments with both = 2
double digest in lane 2, fragments with both = 3
double digest in lane 2, fragments with both = 2
178.
The graph shows the temperature profile of two PCR cycles, starting with 2 DNA molecule(s). Which option is correct?
The graph shows the temperature profile of two PCR cycles, starting with 2 DNA molecule(s). Which option is correct?
step X: extension by Taq polymerase, copies after 6 cycles = 128, time for 6 cycles = 14 min
step X: denaturation, copies after 6 cycles = 64, time for 6 cycles = 20 min
step X: extension by Taq polymerase, copies after 6 cycles = 24, time for 6 cycles = 14 min
step X: primer annealing, copies after 6 cycles = 128, time for 6 cycles = 14 min
179.
A linear DNA of 5000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A linear DNA of 5000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 3, fragments with both = 4
double digest in lane 2, fragments with both = 3
double digest in lane 3, fragments with both = 3
double digest in lane 1, fragments with both = 3
180.
The graph shows the temperature profile of two PCR cycles, starting with 2 DNA molecule(s). Which option is correct?
The graph shows the temperature profile of two PCR cycles, starting with 2 DNA molecule(s). Which option is correct?
step X: primer annealing, copies after 4 cycles = 32, time for 4 cycles = 13.3 min
step X: extension by Taq polymerase, copies after 4 cycles = 32, time for 4 cycles = 13.3 min
step X: denaturation, copies after 4 cycles = 18, time for 4 cycles = 6.67 min
step X: primer annealing, copies after 4 cycles = 32, time for 4 cycles = 6.67 min
181.
A linear DNA of 5000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A linear DNA of 5000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 1, fragments with both = 5
double digest in lane 2, fragments with both = 4
double digest in lane 3, fragments with both = 6
double digest in lane 2, fragments with both = 6
182.
A linear DNA of 8000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A linear DNA of 8000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 2, fragments with both = 4
double digest in lane 3, fragments with both = 5
double digest in lane 1, fragments with both = 4
double digest in lane 2, fragments with both = 6
183.
A linear DNA of 4000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A linear DNA of 4000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 2, fragments with both = 4
double digest in lane 3, fragments with both = 3
double digest in lane 2, fragments with both = 2
double digest in lane 3, fragments with both = 4
184.
A linear DNA of 5000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A linear DNA of 5000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 2, fragments with both = 6
double digest in lane 3, fragments with both = 6
double digest in lane 1, fragments with both = 5
double digest in lane 3, fragments with both = 5
185.
A circular plasmid of 3000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A circular plasmid of 3000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 1, fragments with both = 3
double digest in lane 3, fragments with both = 4
double digest in lane 3, fragments with both = 3
double digest in lane 2, fragments with both = 3
186.
A linear DNA of 4000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A linear DNA of 4000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 1, fragments with both = 5
double digest in lane 2, fragments with both = 4
double digest in lane 1, fragments with both = 6
double digest in lane 2, fragments with both = 5
187.
The graph shows the temperature profile of two PCR cycles, starting with 10 DNA molecule(s). Which option is correct?
The graph shows the temperature profile of two PCR cycles, starting with 10 DNA molecule(s). Which option is correct?
step X: denaturation, copies after 10 cycles = 10240, time for 10 cycles = 21.7 min
step X: denaturation, copies after 10 cycles = 1034, time for 10 cycles = 2.17 min
step X: primer annealing, copies after 10 cycles = 200, time for 10 cycles = 31.7 min
step X: denaturation, copies after 10 cycles = 10240, time for 10 cycles = 43.3 min
188.
A linear DNA of 5000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A linear DNA of 5000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 1, fragments with both = 4
double digest in lane 2, fragments with both = 5
double digest in lane 1, fragments with both = 3
double digest in lane 3, fragments with both = 4
189.
A linear DNA of 4000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A linear DNA of 4000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 2, fragments with both = 6
double digest in lane 3, fragments with both = 4
double digest in lane 3, fragments with both = 5
double digest in lane 1, fragments with both = 6
190.
A circular plasmid of 8000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A circular plasmid of 8000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 3, fragments with both = 4
double digest in lane 3, fragments with both = 3
double digest in lane 1, fragments with both = 4
double digest in lane 2, fragments with both = 5
191.
A linear DNA of 5000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A linear DNA of 5000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 2, fragments with both = 4
double digest in lane 2, fragments with both = 3
double digest in lane 3, fragments with both = 4
double digest in lane 1, fragments with both = 5
192.
A circular plasmid of 4000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A circular plasmid of 4000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 2, fragments with both = 5
double digest in lane 3, fragments with both = 4
double digest in lane 2, fragments with both = 3
double digest in lane 1, fragments with both = 3
193.
A circular plasmid of 6000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A circular plasmid of 6000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 1, fragments with both = 4
double digest in lane 1, fragments with both = 3
double digest in lane 3, fragments with both = 5
double digest in lane 2, fragments with both = 3
194.
A linear DNA of 3000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A linear DNA of 3000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 1, fragments with both = 4
double digest in lane 2, fragments with both = 5
double digest in lane 1, fragments with both = 6
double digest in lane 1, fragments with both = 5
195.
The graph shows the temperature profile of two PCR cycles, starting with 10 DNA molecule(s). Which option is correct?
The graph shows the temperature profile of two PCR cycles, starting with 10 DNA molecule(s). Which option is correct?
step X: denaturation, copies after 11 cycles = 10240, time for 11 cycles = 44 min
step X: primer annealing, copies after 11 cycles = 20480, time for 11 cycles = 22 min
step X: denaturation, copies after 11 cycles = 220, time for 11 cycles = 2 min
step X: denaturation, copies after 11 cycles = 2058, time for 11 cycles = 2 min
196.
A circular plasmid of 8000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A circular plasmid of 8000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 3, fragments with both = 3
double digest in lane 3, fragments with both = 4
double digest in lane 1, fragments with both = 4
double digest in lane 2, fragments with both = 4
197.
A linear DNA of 6000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A linear DNA of 6000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 3, fragments with both = 5
double digest in lane 2, fragments with both = 5
double digest in lane 3, fragments with both = 6
double digest in lane 1, fragments with both = 6
198.
The graph shows the temperature profile of two PCR cycles, starting with 10 DNA molecule(s). Which option is correct?
The graph shows the temperature profile of two PCR cycles, starting with 10 DNA molecule(s). Which option is correct?
step X: denaturation, copies after 7 cycles = 1280, time for 7 cycles = 11.7 min
step X: primer annealing, copies after 7 cycles = 140, time for 7 cycles = 11.7 min
step X: primer annealing, copies after 7 cycles = 1280, time for 7 cycles = 11.7 min
step X: denaturation, copies after 7 cycles = 1280, time for 7 cycles = 23.3 min
199.
The graph shows the temperature profile of two PCR cycles, starting with 5 DNA molecule(s). Which option is correct?
The graph shows the temperature profile of two PCR cycles, starting with 5 DNA molecule(s). Which option is correct?
step X: extension by Taq polymerase, copies after 9 cycles = 90, time for 9 cycles = 42 min
step X: primer annealing, copies after 9 cycles = 2560, time for 9 cycles = 21 min
step X: primer annealing, copies after 9 cycles = 90, time for 9 cycles = 21 min
step X: primer annealing, copies after 9 cycles = 2560, time for 9 cycles = 30 min
200.
A linear DNA of 3000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A linear DNA of 3000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 2, fragments with both = 4
double digest in lane 3, fragments with both = 3
double digest in lane 3, fragments with both = 4
double digest in lane 3, fragments with both = 5
201.
The graph shows the temperature profile of two PCR cycles, starting with 5 DNA molecule(s). Which option is correct?
The graph shows the temperature profile of two PCR cycles, starting with 5 DNA molecule(s). Which option is correct?
step X: extension by Taq polymerase, copies after 6 cycles = 60, time for 6 cycles = 25 min
step X: denaturation, copies after 6 cycles = 320, time for 6 cycles = 19 min
step X: extension by Taq polymerase, copies after 6 cycles = 160, time for 6 cycles = 38 min
step X: primer annealing, copies after 6 cycles = 320, time for 6 cycles = 19 min
202.
A circular plasmid of 6000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A circular plasmid of 6000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 1, fragments with both = 4
double digest in lane 1, fragments with both = 5
double digest in lane 3, fragments with both = 4
double digest in lane 3, fragments with both = 5
203.
A circular plasmid of 4000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A circular plasmid of 4000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 2, fragments with both = 3
double digest in lane 3, fragments with both = 4
double digest in lane 2, fragments with both = 4
double digest in lane 1, fragments with both = 4
204.
A circular plasmid of 4000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A circular plasmid of 4000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 1, fragments with both = 3
double digest in lane 2, fragments with both = 4
double digest in lane 2, fragments with both = 5
double digest in lane 2, fragments with both = 3
205.
The graph shows the temperature profile of two PCR cycles, starting with 1 DNA molecule(s). Which option is correct?
The graph shows the temperature profile of two PCR cycles, starting with 1 DNA molecule(s). Which option is correct?
step X: denaturation, copies after 8 cycles = 16, time for 8 cycles = 13.3 min
step X: primer annealing, copies after 8 cycles = 16, time for 8 cycles = 13.3 min
step X: primer annealing, copies after 8 cycles = 256, time for 8 cycles = 13.3 min
step X: primer annealing, copies after 8 cycles = 256, time for 8 cycles = 26.7 min
206.
A linear DNA of 6000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A linear DNA of 6000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 1, fragments with both = 4
double digest in lane 2, fragments with both = 5
double digest in lane 3, fragments with both = 5
double digest in lane 1, fragments with both = 3
207.
The graph shows the temperature profile of two PCR cycles, starting with 5 DNA molecule(s). Which option is correct?
The graph shows the temperature profile of two PCR cycles, starting with 5 DNA molecule(s). Which option is correct?
step X: primer annealing, copies after 5 cycles = 37, time for 5 cycles = 23.3 min
step X: extension by Taq polymerase, copies after 5 cycles = 80, time for 5 cycles = 23.3 min
step X: primer annealing, copies after 5 cycles = 37, time for 5 cycles = 16.7 min
step X: denaturation, copies after 5 cycles = 160, time for 5 cycles = 11.7 min
208.
The graph shows the temperature profile of two PCR cycles, starting with 10 DNA molecule(s). Which option is correct?
The graph shows the temperature profile of two PCR cycles, starting with 10 DNA molecule(s). Which option is correct?
step X: denaturation, copies after 12 cycles = 40960, time for 12 cycles = 29 min
step X: primer annealing, copies after 12 cycles = 4106, time for 12 cycles = 41 min
step X: extension by Taq polymerase, copies after 12 cycles = 40960, time for 12 cycles = 2.42 min
step X: primer annealing, copies after 12 cycles = 4106, time for 12 cycles = 29 min
209.
The graph shows the temperature profile of two PCR cycles, starting with 3 DNA molecule(s). Which option is correct?
The graph shows the temperature profile of two PCR cycles, starting with 3 DNA molecule(s). Which option is correct?
step X: denaturation, copies after 7 cycles = 384, time for 7 cycles = 2 min
step X: extension by Taq polymerase, copies after 7 cycles = 42, time for 7 cycles = 14 min
step X: primer annealing, copies after 7 cycles = 384, time for 7 cycles = 14 min
step X: primer annealing, copies after 7 cycles = 131, time for 7 cycles = 28 min
210.
A linear DNA of 4000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A linear DNA of 4000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 2, fragments with both = 3
double digest in lane 2, fragments with both = 5
double digest in lane 1, fragments with both = 3
double digest in lane 2, fragments with both = 4
211.
A linear DNA of 4000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A linear DNA of 4000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 1, fragments with both = 6
double digest in lane 3, fragments with both = 5
double digest in lane 1, fragments with both = 4
double digest in lane 3, fragments with both = 4
212.
A circular plasmid of 3000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A circular plasmid of 3000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 3, fragments with both = 4
double digest in lane 3, fragments with both = 3
double digest in lane 3, fragments with both = 5
double digest in lane 2, fragments with both = 4
213.
A circular plasmid of 6000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A circular plasmid of 6000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 1, fragments with both = 4
double digest in lane 3, fragments with both = 5
double digest in lane 2, fragments with both = 4
double digest in lane 1, fragments with both = 5
214.
A linear DNA of 4000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A linear DNA of 4000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 3, fragments with both = 3
double digest in lane 1, fragments with both = 4
double digest in lane 2, fragments with both = 4
double digest in lane 1, fragments with both = 5
215.
A circular plasmid of 3000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A circular plasmid of 3000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 3, fragments with both = 4
double digest in lane 2, fragments with both = 3
double digest in lane 3, fragments with both = 3
double digest in lane 2, fragments with both = 4
216.
A circular plasmid of 5000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A circular plasmid of 5000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 3, fragments with both = 4
double digest in lane 1, fragments with both = 3
double digest in lane 1, fragments with both = 4
double digest in lane 2, fragments with both = 4
217.
A linear DNA of 4000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A linear DNA of 4000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 2, fragments with both = 3
double digest in lane 2, fragments with both = 4
double digest in lane 2, fragments with both = 5
double digest in lane 1, fragments with both = 4
218.
The graph shows the temperature profile of two PCR cycles, starting with 10 DNA molecule(s). Which option is correct?
The graph shows the temperature profile of two PCR cycles, starting with 10 DNA molecule(s). Which option is correct?
step X: denaturation, copies after 11 cycles = 220, time for 11 cycles = 22 min
step X: primer annealing, copies after 11 cycles = 10240, time for 11 cycles = 2 min
step X: denaturation, copies after 11 cycles = 20480, time for 11 cycles = 22 min
step X: primer annealing, copies after 11 cycles = 20480, time for 11 cycles = 22 min
219.
The graph shows the temperature profile of two PCR cycles, starting with 2 DNA molecule(s). Which option is correct?
The graph shows the temperature profile of two PCR cycles, starting with 2 DNA molecule(s). Which option is correct?
step X: primer annealing, copies after 10 cycles = 40, time for 10 cycles = 35 min
step X: extension by Taq polymerase, copies after 10 cycles = 2048, time for 10 cycles = 17.5 min
step X: extension by Taq polymerase, copies after 10 cycles = 1024, time for 10 cycles = 35 min
step X: denaturation, copies after 10 cycles = 1024, time for 10 cycles = 17.5 min
220.
A linear DNA of 3000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A linear DNA of 3000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 1, fragments with both = 3
double digest in lane 2, fragments with both = 3
double digest in lane 3, fragments with both = 4
double digest in lane 3, fragments with both = 5
221.
The graph shows the temperature profile of two PCR cycles, starting with 10 DNA molecule(s). Which option is correct?
The graph shows the temperature profile of two PCR cycles, starting with 10 DNA molecule(s). Which option is correct?
step X: primer annealing, copies after 4 cycles = 80, time for 4 cycles = 7 min
step X: extension by Taq polymerase, copies after 4 cycles = 160, time for 4 cycles = 7 min
step X: denaturation, copies after 4 cycles = 80, time for 4 cycles = 11 min
step X: extension by Taq polymerase, copies after 4 cycles = 160, time for 4 cycles = 11 min
222.
A linear DNA of 5000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A linear DNA of 5000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 2, fragments with both = 3
double digest in lane 3, fragments with both = 5
double digest in lane 1, fragments with both = 3
double digest in lane 2, fragments with both = 4
223.
The graph shows the temperature profile of two PCR cycles, starting with 3 DNA molecule(s). Which option is correct?
The graph shows the temperature profile of two PCR cycles, starting with 3 DNA molecule(s). Which option is correct?
step X: extension by Taq polymerase, copies after 5 cycles = 48, time for 5 cycles = 12.5 min
step X: primer annealing, copies after 5 cycles = 35, time for 5 cycles = 12.5 min
step X: primer annealing, copies after 5 cycles = 96, time for 5 cycles = 12.5 min
step X: extension by Taq polymerase, copies after 5 cycles = 96, time for 5 cycles = 12.5 min
224.
A linear DNA of 8000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A linear DNA of 8000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 2, fragments with both = 4
double digest in lane 2, fragments with both = 3
double digest in lane 3, fragments with both = 4
double digest in lane 1, fragments with both = 5
225.
A linear DNA of 8000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A linear DNA of 8000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 3, fragments with both = 4
double digest in lane 3, fragments with both = 6
double digest in lane 3, fragments with both = 5
double digest in lane 1, fragments with both = 6
226.
A circular plasmid of 4000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A circular plasmid of 4000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 3, fragments with both = 2
double digest in lane 1, fragments with both = 4
double digest in lane 2, fragments with both = 2
double digest in lane 3, fragments with both = 3
227.
A linear DNA of 4000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A linear DNA of 4000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 3, fragments with both = 4
double digest in lane 2, fragments with both = 5
double digest in lane 2, fragments with both = 3
double digest in lane 2, fragments with both = 4
228.
The graph shows the temperature profile of two PCR cycles, starting with 5 DNA molecule(s). Which option is correct?
The graph shows the temperature profile of two PCR cycles, starting with 5 DNA molecule(s). Which option is correct?
step X: extension by Taq polymerase, copies after 5 cycles = 160, time for 5 cycles = 13.3 min
step X: denaturation, copies after 5 cycles = 80, time for 5 cycles = 8.33 min
step X: denaturation, copies after 5 cycles = 160, time for 5 cycles = 8.33 min
step X: extension by Taq polymerase, copies after 5 cycles = 50, time for 5 cycles = 8.33 min
229.
A linear DNA of 3000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A linear DNA of 3000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 2, fragments with both = 4
double digest in lane 3, fragments with both = 2
double digest in lane 1, fragments with both = 3
double digest in lane 1, fragments with both = 2
230.
The graph shows the temperature profile of two PCR cycles, starting with 10 DNA molecule(s). Which option is correct?
The graph shows the temperature profile of two PCR cycles, starting with 10 DNA molecule(s). Which option is correct?
step X: extension by Taq polymerase, copies after 9 cycles = 5120, time for 9 cycles = 1.83 min
step X: extension by Taq polymerase, copies after 9 cycles = 2560, time for 9 cycles = 25.5 min
step X: denaturation, copies after 9 cycles = 5120, time for 9 cycles = 16.5 min
step X: denaturation, copies after 9 cycles = 2560, time for 9 cycles = 33 min
231.
A linear DNA of 8000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A linear DNA of 8000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 2, fragments with both = 3
double digest in lane 3, fragments with both = 4
double digest in lane 1, fragments with both = 4
double digest in lane 2, fragments with both = 5
232.
A linear DNA of 4000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A linear DNA of 4000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 3, fragments with both = 3
double digest in lane 3, fragments with both = 4
double digest in lane 1, fragments with both = 3
double digest in lane 2, fragments with both = 3
233.
A circular plasmid of 5000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A circular plasmid of 5000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 1, fragments with both = 1
double digest in lane 3, fragments with both = 2
double digest in lane 2, fragments with both = 2
double digest in lane 3, fragments with both = 3
234.
The graph shows the temperature profile of two PCR cycles, starting with 3 DNA molecule(s). Which option is correct?
The graph shows the temperature profile of two PCR cycles, starting with 3 DNA molecule(s). Which option is correct?
step X: extension by Taq polymerase, copies after 8 cycles = 768, time for 8 cycles = 20.7 min
step X: extension by Taq polymerase, copies after 8 cycles = 768, time for 8 cycles = 41.3 min
step X: extension by Taq polymerase, copies after 8 cycles = 48, time for 8 cycles = 2.58 min
step X: denaturation, copies after 8 cycles = 768, time for 8 cycles = 20.7 min
235.
The graph shows the temperature profile of two PCR cycles, starting with 2 DNA molecule(s). Which option is correct?
The graph shows the temperature profile of two PCR cycles, starting with 2 DNA molecule(s). Which option is correct?
step X: extension by Taq polymerase, copies after 5 cycles = 32, time for 5 cycles = 8.33 min
step X: extension by Taq polymerase, copies after 5 cycles = 64, time for 5 cycles = 13.3 min
step X: extension by Taq polymerase, copies after 5 cycles = 64, time for 5 cycles = 8.33 min
step X: denaturation, copies after 5 cycles = 34, time for 5 cycles = 13.3 min
236.
A circular plasmid of 6000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A circular plasmid of 6000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 3, fragments with both = 4
double digest in lane 1, fragments with both = 3
double digest in lane 3, fragments with both = 2
double digest in lane 2, fragments with both = 3
237.
A linear DNA of 4000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A linear DNA of 4000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 3, fragments with both = 4
double digest in lane 2, fragments with both = 6
double digest in lane 1, fragments with both = 5
double digest in lane 3, fragments with both = 5
238.
A linear DNA of 8000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A linear DNA of 8000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 1, fragments with both = 4
double digest in lane 3, fragments with both = 3
double digest in lane 2, fragments with both = 3
double digest in lane 2, fragments with both = 2
239.
A linear DNA of 6000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A linear DNA of 6000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 2, fragments with both = 4
double digest in lane 1, fragments with both = 4
double digest in lane 3, fragments with both = 4
double digest in lane 2, fragments with both = 3
240.
A circular plasmid of 6000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A circular plasmid of 6000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 2, fragments with both = 4
double digest in lane 3, fragments with both = 4
double digest in lane 1, fragments with both = 5
double digest in lane 2, fragments with both = 3
241.
A linear DNA of 5000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A linear DNA of 5000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 3, fragments with both = 3
double digest in lane 3, fragments with both = 5
double digest in lane 2, fragments with both = 4
double digest in lane 3, fragments with both = 4
242.
A circular plasmid of 3000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A circular plasmid of 3000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 2, fragments with both = 3
double digest in lane 1, fragments with both = 3
double digest in lane 3, fragments with both = 2
double digest in lane 2, fragments with both = 2
243.
A linear DNA of 6000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A linear DNA of 6000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 3, fragments with both = 5
double digest in lane 2, fragments with both = 6
double digest in lane 1, fragments with both = 6
double digest in lane 1, fragments with both = 4
244.
A circular plasmid of 8000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A circular plasmid of 8000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 1, fragments with both = 2
double digest in lane 2, fragments with both = 2
double digest in lane 1, fragments with both = 3
double digest in lane 2, fragments with both = 3
245.
A linear DNA of 8000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A linear DNA of 8000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 3, fragments with both = 4
double digest in lane 3, fragments with both = 5
double digest in lane 1, fragments with both = 5
double digest in lane 2, fragments with both = 5
246.
A circular plasmid of 6000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A circular plasmid of 6000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 1, fragments with both = 4
double digest in lane 3, fragments with both = 3
double digest in lane 3, fragments with both = 4
double digest in lane 3, fragments with both = 2
247.
A circular plasmid of 3000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A circular plasmid of 3000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 3, fragments with both = 2
double digest in lane 1, fragments with both = 2
double digest in lane 2, fragments with both = 1
double digest in lane 3, fragments with both = 1
248.
The graph shows the temperature profile of two PCR cycles, starting with 10 DNA molecule(s). Which option is correct?
The graph shows the temperature profile of two PCR cycles, starting with 10 DNA molecule(s). Which option is correct?
step X: extension by Taq polymerase, copies after 11 cycles = 20480, time for 11 cycles = 23.8 min
step X: primer annealing, copies after 11 cycles = 20480, time for 11 cycles = 34.8 min
step X: primer annealing, copies after 11 cycles = 2058, time for 11 cycles = 23.8 min
step X: denaturation, copies after 11 cycles = 20480, time for 11 cycles = 23.8 min
249.
A linear DNA of 8000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A linear DNA of 8000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 3, fragments with both = 5
double digest in lane 2, fragments with both = 5
double digest in lane 2, fragments with both = 4
double digest in lane 1, fragments with both = 5
250.
A circular plasmid of 3000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A circular plasmid of 3000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 2, fragments with both = 3
double digest in lane 2, fragments with both = 2
double digest in lane 3, fragments with both = 2
double digest in lane 3, fragments with both = 4
251.
The graph shows the temperature profile of two PCR cycles, starting with 5 DNA molecule(s). Which option is correct?
The graph shows the temperature profile of two PCR cycles, starting with 5 DNA molecule(s). Which option is correct?
step X: denaturation, copies after 9 cycles = 517, time for 9 cycles = 57 min
step X: primer annealing, copies after 9 cycles = 2560, time for 9 cycles = 37.5 min
step X: denaturation, copies after 9 cycles = 90, time for 9 cycles = 28.5 min
step X: extension by Taq polymerase, copies after 9 cycles = 2560, time for 9 cycles = 28.5 min
252.
A linear DNA of 3000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A linear DNA of 3000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 1, fragments with both = 4
double digest in lane 2, fragments with both = 3
double digest in lane 2, fragments with both = 4
double digest in lane 1, fragments with both = 3
253.
A linear DNA of 3000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A linear DNA of 3000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 1, fragments with both = 6
double digest in lane 1, fragments with both = 5
double digest in lane 2, fragments with both = 5
double digest in lane 2, fragments with both = 6
254.
A linear DNA of 4000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A linear DNA of 4000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 1, fragments with both = 3
double digest in lane 2, fragments with both = 2
double digest in lane 3, fragments with both = 2
double digest in lane 3, fragments with both = 4
255.
The graph shows the temperature profile of two PCR cycles, starting with 5 DNA molecule(s). Which option is correct?
The graph shows the temperature profile of two PCR cycles, starting with 5 DNA molecule(s). Which option is correct?
step X: extension by Taq polymerase, copies after 9 cycles = 90, time for 9 cycles = 18.8 min
step X: primer annealing, copies after 9 cycles = 2560, time for 9 cycles = 37.5 min
step X: primer annealing, copies after 9 cycles = 2560, time for 9 cycles = 18.8 min
step X: extension by Taq polymerase, copies after 9 cycles = 90, time for 9 cycles = 27.8 min
256.
A circular plasmid of 4000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A circular plasmid of 4000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 1, fragments with both = 5
double digest in lane 2, fragments with both = 3
double digest in lane 1, fragments with both = 4
double digest in lane 3, fragments with both = 3
257.
A circular plasmid of 8000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A circular plasmid of 8000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 2, fragments with both = 2
double digest in lane 2, fragments with both = 4
double digest in lane 1, fragments with both = 3
double digest in lane 3, fragments with both = 3
258.
A circular plasmid of 6000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A circular plasmid of 6000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 1, fragments with both = 2
double digest in lane 2, fragments with both = 1
double digest in lane 2, fragments with both = 3
double digest in lane 3, fragments with both = 2
259.
A linear DNA of 5000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A linear DNA of 5000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 2, fragments with both = 4
double digest in lane 3, fragments with both = 3
double digest in lane 1, fragments with both = 4
double digest in lane 1, fragments with both = 3
260.
A circular plasmid of 5000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A circular plasmid of 5000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 3, fragments with both = 4
double digest in lane 2, fragments with both = 4
double digest in lane 3, fragments with both = 5
double digest in lane 2, fragments with both = 5
261.
A circular plasmid of 5000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A circular plasmid of 5000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 3, fragments with both = 5
double digest in lane 3, fragments with both = 4
double digest in lane 1, fragments with both = 4
double digest in lane 2, fragments with both = 4
262.
A circular plasmid of 8000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A circular plasmid of 8000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 1, fragments with both = 4
double digest in lane 1, fragments with both = 5
double digest in lane 2, fragments with both = 3
double digest in lane 3, fragments with both = 4
263.
The graph shows the temperature profile of two PCR cycles, starting with 10 DNA molecule(s). Which option is correct?
The graph shows the temperature profile of two PCR cycles, starting with 10 DNA molecule(s). Which option is correct?
step X: extension by Taq polymerase, copies after 7 cycles = 640, time for 7 cycles = 25.7 min
step X: denaturation, copies after 7 cycles = 138, time for 7 cycles = 19.8 min
step X: denaturation, copies after 7 cycles = 1280, time for 7 cycles = 12.8 min
step X: primer annealing, copies after 7 cycles = 1280, time for 7 cycles = 12.8 min
264.
A circular plasmid of 3000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A circular plasmid of 3000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 3, fragments with both = 2
double digest in lane 3, fragments with both = 4
double digest in lane 1, fragments with both = 4
double digest in lane 2, fragments with both = 3
265.
A linear DNA of 6000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A linear DNA of 6000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 2, fragments with both = 4
double digest in lane 2, fragments with both = 5
double digest in lane 1, fragments with both = 6
double digest in lane 2, fragments with both = 6
266.
A circular plasmid of 3000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A circular plasmid of 3000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 2, fragments with both = 3
double digest in lane 1, fragments with both = 4
double digest in lane 3, fragments with both = 3
double digest in lane 2, fragments with both = 2
267.
A linear DNA of 4000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A linear DNA of 4000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 2, fragments with both = 4
double digest in lane 1, fragments with both = 5
double digest in lane 2, fragments with both = 5
double digest in lane 1, fragments with both = 4
268.
The graph shows the temperature profile of two PCR cycles, starting with 1 DNA molecule(s). Which option is correct?
The graph shows the temperature profile of two PCR cycles, starting with 1 DNA molecule(s). Which option is correct?
step X: denaturation, copies after 5 cycles = 32, time for 5 cycles = 10 min
step X: denaturation, copies after 5 cycles = 16, time for 5 cycles = 20 min
step X: primer annealing, copies after 5 cycles = 33, time for 5 cycles = 15 min
step X: extension by Taq polymerase, copies after 5 cycles = 10, time for 5 cycles = 10 min
269.
A circular plasmid of 4000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A circular plasmid of 4000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 1, fragments with both = 2
double digest in lane 2, fragments with both = 3
double digest in lane 2, fragments with both = 4
double digest in lane 2, fragments with both = 2
270.
A linear DNA of 4000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A linear DNA of 4000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 1, fragments with both = 5
double digest in lane 2, fragments with both = 6
double digest in lane 3, fragments with both = 4
double digest in lane 2, fragments with both = 4
271.
A linear DNA of 5000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A linear DNA of 5000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 2, fragments with both = 5
double digest in lane 2, fragments with both = 3
double digest in lane 1, fragments with both = 4
double digest in lane 2, fragments with both = 4
272.
A linear DNA of 3000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A linear DNA of 3000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 2, fragments with both = 4
double digest in lane 1, fragments with both = 6
double digest in lane 1, fragments with both = 5
double digest in lane 2, fragments with both = 6
273.
A linear DNA of 8000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A linear DNA of 8000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 1, fragments with both = 4
double digest in lane 2, fragments with both = 5
double digest in lane 3, fragments with both = 5
double digest in lane 2, fragments with both = 4
274.
A circular plasmid of 3000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A circular plasmid of 3000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 3, fragments with both = 2
double digest in lane 2, fragments with both = 3
double digest in lane 1, fragments with both = 1
double digest in lane 3, fragments with both = 3
275.
A circular plasmid of 8000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A circular plasmid of 8000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 2, fragments with both = 5
double digest in lane 2, fragments with both = 4
double digest in lane 3, fragments with both = 4
double digest in lane 3, fragments with both = 3
276.
A linear DNA of 4000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A linear DNA of 4000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 1, fragments with both = 3
double digest in lane 2, fragments with both = 3
double digest in lane 1, fragments with both = 4
double digest in lane 1, fragments with both = 5
277.
A circular plasmid of 5000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A circular plasmid of 5000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 3, fragments with both = 4
double digest in lane 2, fragments with both = 4
double digest in lane 3, fragments with both = 3
double digest in lane 1, fragments with both = 3
278.
A linear DNA of 8000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A linear DNA of 8000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 3, fragments with both = 4
double digest in lane 2, fragments with both = 4
double digest in lane 2, fragments with both = 5
double digest in lane 1, fragments with both = 3
279.
A linear DNA of 4000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A linear DNA of 4000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 1, fragments with both = 4
double digest in lane 2, fragments with both = 5
double digest in lane 3, fragments with both = 5
double digest in lane 3, fragments with both = 4
280.
A linear DNA of 6000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A linear DNA of 6000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 1, fragments with both = 6
double digest in lane 2, fragments with both = 6
double digest in lane 3, fragments with both = 5
double digest in lane 2, fragments with both = 5
281.
A linear DNA of 4000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A linear DNA of 4000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 3, fragments with both = 5
double digest in lane 1, fragments with both = 5
double digest in lane 2, fragments with both = 6
double digest in lane 3, fragments with both = 6
282.
A circular plasmid of 5000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A circular plasmid of 5000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 3, fragments with both = 3
double digest in lane 3, fragments with both = 4
double digest in lane 2, fragments with both = 4
double digest in lane 1, fragments with both = 4
283.
A circular plasmid of 8000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A circular plasmid of 8000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 3, fragments with both = 5
double digest in lane 1, fragments with both = 4
double digest in lane 2, fragments with both = 3
double digest in lane 2, fragments with both = 4
284.
A linear DNA of 6000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A linear DNA of 6000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 1, fragments with both = 5
double digest in lane 1, fragments with both = 4
double digest in lane 3, fragments with both = 5
double digest in lane 2, fragments with both = 6
285.
A linear DNA of 3000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A linear DNA of 3000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 3, fragments with both = 5
double digest in lane 2, fragments with both = 4
double digest in lane 2, fragments with both = 3
double digest in lane 1, fragments with both = 4
286.
A linear DNA of 6000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A linear DNA of 6000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 3, fragments with both = 5
double digest in lane 2, fragments with both = 4
double digest in lane 1, fragments with both = 5
double digest in lane 3, fragments with both = 3
287.
A linear DNA of 8000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A linear DNA of 8000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 2, fragments with both = 4
double digest in lane 1, fragments with both = 4
double digest in lane 2, fragments with both = 3
double digest in lane 1, fragments with both = 5
288.
A linear DNA of 6000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A linear DNA of 6000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 2, fragments with both = 5
double digest in lane 1, fragments with both = 5
double digest in lane 1, fragments with both = 3
double digest in lane 2, fragments with both = 4
289.
A circular plasmid of 5000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A circular plasmid of 5000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 3, fragments with both = 2
double digest in lane 2, fragments with both = 4
double digest in lane 2, fragments with both = 3
double digest in lane 2, fragments with both = 2
290.
A circular plasmid of 4000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A circular plasmid of 4000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 2, fragments with both = 3
double digest in lane 3, fragments with both = 2
double digest in lane 3, fragments with both = 3
double digest in lane 1, fragments with both = 3
291.
A circular plasmid of 3000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A circular plasmid of 3000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 3, fragments with both = 3
double digest in lane 1, fragments with both = 2
double digest in lane 3, fragments with both = 2
double digest in lane 2, fragments with both = 3
292.
A linear DNA of 8000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A linear DNA of 8000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 1, fragments with both = 5
double digest in lane 1, fragments with both = 4
double digest in lane 3, fragments with both = 3
double digest in lane 3, fragments with both = 4
293.
A circular plasmid of 5000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A circular plasmid of 5000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 3, fragments with both = 3
double digest in lane 1, fragments with both = 3
double digest in lane 1, fragments with both = 2
double digest in lane 3, fragments with both = 2
294.
A linear DNA of 5000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A linear DNA of 5000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 3, fragments with both = 3
double digest in lane 2, fragments with both = 2
double digest in lane 2, fragments with both = 3
double digest in lane 3, fragments with both = 4
295.
The graph shows the temperature profile of two PCR cycles, starting with 5 DNA molecule(s). Which option is correct?
The graph shows the temperature profile of two PCR cycles, starting with 5 DNA molecule(s). Which option is correct?
step X: primer annealing, copies after 12 cycles = 20480, time for 12 cycles = 72 min
step X: primer annealing, copies after 12 cycles = 20480, time for 12 cycles = 48 min
step X: primer annealing, copies after 12 cycles = 20480, time for 12 cycles = 36 min
step X: denaturation, copies after 12 cycles = 20480, time for 12 cycles = 3 min
296.
A linear DNA of 4000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A linear DNA of 4000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 2, fragments with both = 4
double digest in lane 2, fragments with both = 3
double digest in lane 1, fragments with both = 4
double digest in lane 3, fragments with both = 5
297.
The graph shows the temperature profile of two PCR cycles, starting with 5 DNA molecule(s). Which option is correct?
The graph shows the temperature profile of two PCR cycles, starting with 5 DNA molecule(s). Which option is correct?
step X: primer annealing, copies after 8 cycles = 80, time for 8 cycles = 25.3 min
step X: extension by Taq polymerase, copies after 8 cycles = 1280, time for 8 cycles = 17.3 min
step X: denaturation, copies after 8 cycles = 1280, time for 8 cycles = 17.3 min
step X: primer annealing, copies after 8 cycles = 80, time for 8 cycles = 34.7 min
298.
A linear DNA of 8000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A linear DNA of 8000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 2, fragments with both = 4
double digest in lane 1, fragments with both = 5
double digest in lane 1, fragments with both = 6
double digest in lane 3, fragments with both = 5
299.
A linear DNA of 3000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A linear DNA of 3000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 1, fragments with both = 3
double digest in lane 3, fragments with both = 4
double digest in lane 1, fragments with both = 4
double digest in lane 1, fragments with both = 5
300.
The graph shows the temperature profile of two PCR cycles, starting with 3 DNA molecule(s). Which option is correct?
The graph shows the temperature profile of two PCR cycles, starting with 3 DNA molecule(s). Which option is correct?
step X: extension by Taq polymerase, copies after 4 cycles = 24, time for 4 cycles = 18 min
step X: denaturation, copies after 4 cycles = 19, time for 4 cycles = 13 min
step X: extension by Taq polymerase, copies after 4 cycles = 48, time for 4 cycles = 9 min
step X: primer annealing, copies after 4 cycles = 19, time for 4 cycles = 9 min
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