Worked example 10 — design a cloning experiment
Point 8 of 12 — Lesson 2: Recombinant DNA, Gene Cloning, PCR and Genetic EngineeringQuestion bank: 32 questions (32 with pictures)
08 Worked example 10 — design a cloning experiment
- Aim: produce many copies of a human gene.
- Cut human DNA and a bacterial plasmid with the same restriction enzyme (e.g.
- one with a 6-nucleotide site); mix, ligate; add to bacteria; grow culture; collect plasmids; cut them with the same enzyme to release the gene.
- Why the same enzyme?
- Because it gives identical sticky ends that can pair.
📝 Question bank for this point
32 questions linked to this point only, 32 of them with pictures.