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7.17 · Transport in Plants

Structure of Transport Tissues — Cambridge AS & A Level Biology 9700 Revision Notes & Topical Questions

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7.1 Structure of Transport Tissues — CAMBRIDGE AS & A LEVEL BIOLOGY 9700 — REVISION NOTES notes: Describe the distribution of xylem and phloem in dicotyledonous roots, stems and leaves, relate xylem and phloem cell structure to function and describe plan diagrams.

  • Vascular tissue
  • Dicotyledonous plants
  • Herbaceous
  • Dead, with no contents
  • No end walls
  • Lignified walls

Section 1 of 10Why plants need transport systems

Why plants need transport systems
Size
  • Size plants have many layers of cells and a small SA:V ratio, so diffusion is too slow.
Movement
  • Movement water and mineral ions go up from the roots, and sugars go from leaves to where they are used or stored.
Vascular tissue
  • Vascular tissue xylem and phloem lie together in vascular bundles in the root, stem and leaf.
Dicotyledonous plants
  • Dicotyledonous plants they have seeds with two cotyledons, leaves with a branching network of veins, a broad blade with a stalk (petiole) and a tap root with side branches.
Herbaceous
  • Herbaceous herbaceous dicots are non-woody and often live for one growing season.

Section 2 of 10Position of xylem and phloem

Organ (dicot)XylemPhloemArrangement
RootCentral, star or X-shapedBetween the arms of the xylemCentral vascular cylinder surrounded by pericycle and endodermis; central position resists pulling forces
StemInner side of bundles near the centreOuter side of bundles, with cambium betweenRing of bundles near the outside, which resists bending
LeafUpper (adaxial) side of the midrib and veinsLower side of the midrib and veinsMidrib bundle with veins branching through the mesophyll

Section 3 of 10Xylem vessel elements

Dead, with no contents
  • Dead, with no contents nothing impedes the flow of water.
No end walls
  • No end walls they form a continuous column of water.
Lignified walls
  • Lignified walls lignin is strong and waterproof, preventing collapse under negative pressure.
Pits
  • Pits they allow lateral movement and bypass of air bubbles.
Narrow lumen
  • Narrow lumen it increases adhesion of water to the walls and helps capillarity.
Other xylem cells
  • Other xylem cells tracheids, fibres (support) and parenchyma.
Transport
  • Transport water moves by mass flow under tension, down a water potential gradient.
    • (cohesion–tension)

Section 4 of 10Phloem sieve tube elements and companion cells

Sieve tube elements
  • Sieve tube elements they have little cytoplasm, no nucleus and few organelles, so sap can flow.
  • Sieve plates with pores let sap pass and can be sealed by callose if damaged.
Companion cells
  • Companion cells they have a nucleus, many mitochondria and ribosomes.
  • They supply ATP and proteins to the sieve elements through plasmodesmata and actively load sucrose by proton–sucrose co-transport.
Transport
  • Transport sucrose moves by mass flow from source to sink (low pressure).
    • (high hydrostatic pressure)

Section 5 of 10Xylem and phloem compared

FeatureXylem vessel elementPhloem sieve tube element
Living?DeadLiving
Cell contentsNone, hollow lumenThin layer of cytoplasm; no nucleus
End wallsAbsent, so a continuous tubeSieve plates
WallsLignified, with pitsCellulose, not lignified
TransportsWater and mineral ions, upwards onlySucrose and amino acids, up or down
Associated cellNoneCompanion cell
  • Size
    • xylem vessels are typically 20 to 200 µm in diameter and sieve tubes 10 to 40 µm.

Section 6 of 10Xerophyte adaptations

Xerophyte adaptations
Aim
  • Aim they reduce the water vapour potential gradient or evaporation.
Features
  • Features thick waxy cuticle, rolled leaves, sunken stomata in pits, hairs that trap moist air, fewer stomata and reduced leaf area (spines).
Marram grass
  • Marram grass leaves roll with stomata on the inner surface, with hinge cells, hairs and sunken stomata.
Cacti
  • Cacti stems store water and leaves are reduced to spines.

Section 7 of 10Plan diagrams and sections

1
Cut a thin transverse section of stem or root with a sharp blade, or use a prepared slide.
2
Mount it in water, optionally stained with toluidine blue (lignin stains blue-green), and add a coverslip.
3
View under low power to find the vascular bundles and their arrangement.
4
Draw a plan diagram showing tissue outlines only, with no individual cells, using sharp continuous lines and correct proportions.
5
Switch to high power and draw a few cells, such as a xylem vessel, sieve tube and companion cell, including cell walls.
6
Label with ruled lines that touch the structure and do not cross, and add the magnification or scale.
Magnification
Magnification image size ÷ actual size, so actual size = image size ÷ magnification, with both in the same units (1 mm = 1000 µm; 1 µm = 1000 nm).
Graticule
  • Graticule the value of one eyepiece division = stage micrometer length ÷ number of eyepiece divisions.

Section 8 of 10More from the specification

More from the specification
Phloem structure and translocation of assimilates
  • Phloem sieve tube elements transport organic assimilates, predominantly sucrose synthesised from glucose,
  • as dissolved solutes to growing and storage parts of the plant.
Xylem tissue and structural features
  • Mature xylem vessel elements are dead, hollow tubes with lignified walls that provide mechanical support and conduct water and dissolved mineral ions upwards from roots.
Micrograph interpretation and biological drawing
  • In examination drawing tasks, students must identify tissue types from a light micrograph or plan diagram, using sharp, unbroken lines without shading to represent vascular bundles.
Transverse sections of plant transport organs
  • Plant stems and roots contain distinct vascular bundles arranged with xylem and phloem, specialised to transport organic compounds and inorganic substances efficiently.
Assimilates
  • Organic substances such as sucrose and amino acids transported in the phloem.
Micrograph
  • A photograph taken through a microscope.
Compound
  • A substance of two or more kinds of atoms chemically combined
Glucose
  • A simple sugar; the unit that starch, glycogen and cellulose are built from.
Organic compound
  • Compound containing carbon and hydrogen.
Transport system
  • A system, such as the circulatory system, that moves substances around the body.

Section 9 of 10Key terms

TermMeaning
Xylem vessel elementA dead, lignified cell with no end walls, joined end to end to form a hollow vessel.
LigninA strong, waterproof polymer deposited in xylem cell walls.
Sieve plateA perforated end wall between sieve tube elements.
Companion cellA cell next to a sieve tube element that supports it and actively loads sucrose.
PlasmodesmataChannels through plant cell walls linking the cytoplasm of adjacent cells.
Vascular bundleA strand of transport tissue containing xylem and phloem.
TranslocationMovement of assimilates in the phloem from sources to sinks.
XerophyteA plant adapted to dry conditions by reducing water loss.

Section 10 of 10Exam technique

1
Putting the xylem on the outside of the phloem in a stem bundle — xylem is on the inner side.
2
Saying phloem sieve tubes are dead — they are living cells.
3
Drawing individual cells in a plan diagram — it shows tissue outlines only.
4
Saying the root has pith — a dicot root has a solid central vascular cylinder.

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