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8.1.7Transport in Plants

Translocation — Cambridge IGCSE Co-ordinated Sciences 0654 - Biology Revision Notes & Topical Questions

5 min read 9 parts Lesson 8 of 8

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Part 1 of 7What is translocation?

Translocation is the movement of dissolved food, mainly sucrose, from the leaves where it is made to other parts of the plant.

What is translocation?
The sugar travels in the phloem tissue.
Plants make glucose in photosynthesis,
  • but it is converted to sucrose for transport because sucrose is more soluble and less likely to be used in reactions on the way.
Amino acids made from nitrate and glucose are also transported in the phloem.

Part 2 of 7Sources and sinks

The phloem moves substances from a source, a part of the plant that makes or releases sugar, to a sink, a part that uses or stores it. Leaves are the main source. Sinks include:

Sources and sinks
Growing regions such as shoot tips and root tips, which use sucrose for respiration and to build new cells.
Storage organs, such as potato tubers or carrot roots, where sucrose is converted to starch.
Developing fruits and seeds.

The roles can change during the year. A potato tuber is a sink while the plant is growing, but in spring it becomes a source when stored food is used to grow new shoots.

Part 3 of 7The structure of phloem

Phloem is made of living cells. Sieve tube elements are joined end to end, with perforated end walls called sieve plates through which dissolved food can flow.

The structure of phloem
They have very little cytoplasm and no nucleus, which makes the pathway open.
Companion cell
Each sieve tube element is linked to a companion cell by narrow channels.
The companion cell has a nucleus, dense cytoplasm and many mitochondria,
  • so it carries out the metabolism for both cells and supplies the energy to load sucrose into the sieve tube by active transport.

Part 4 of 7How translocation works

Because translocation needs energy, it is an active process, unlike transpiration. In outline:

1
Sucrose is moved from the photosynthesising cells into the phloem at the source using energy from respiration.
2
The high solute concentration lowers the water potential in the phloem, so water enters by osmosis from the nearby xylem.
3
The water raises the pressure, which pushes the sap along the sieve tubes towards the sink, where the pressure is lower.
4
At the sink the sucrose is removed and used or stored, and water returns to the xylem.

This is the mass flow idea. The sap can move in both directions in a plant, but in a given tube it flows in one direction at a time.

Part 5 of 7Comparing the two transport streams

FeatureTranspiration (xylem)Translocation (phloem)
SubstanceWater and mineral ionsSucrose and amino acids
DirectionUpwards, from roots to leavesFrom sources to sinks, up or down
TissueDead xylem vesselsLiving sieve tubes and companion cells
Energy neededNo energy from the plant; driven by evaporationYes, energy needed for loading

Part 6 of 7Evidence and investigation

Scientists have shown that sap flows in the phloem using aphids.

1
An aphid inserts its feeding tube into a sieve tube, and the sap is forced out under pressure through the tube when the aphid is removed.
2
Analysis shows it is rich in sucrose.
3
Radioactively labelled carbon dioxide, supplied to a leaf, appears in the phloem as labelled sucrose and can be followed to the sinks.
4
If a ring of bark containing the phloem is removed from a stem, sugar collects above the ring and the tissues below it eventually die,
  • which shows that phloem carries sugar downwards.
5
Metabolic poisons that stop respiration also stop translocation, showing it needs energy.

Uses of the sugar that arrives

What happens to sucrose at the sink depends on the cell.

1
In growing tissues it is used for respiration to provide energy and as a raw material to make cellulose for cell walls.
2
In storage organs, such as a potato tuber, it is converted to insoluble starch,
  • which does not affect water potential in the cells,
  • so it can be stored in large amounts without drawing in water.
3
In fruits it may be stored as sugar to attract animals that disperse the seeds.
4
In spring, enzymes break the starch back down to sugar, and the phloem moves it to the growing buds,
  • which is why translocation can go upwards as well as downwards.

Part 7 of 7Exam-style questions

  1. Describe the function of the phloem and explain how it is adapted for this function. [4]4 marks

  2. Give two differences between transpiration and translocation. [2]2 marks

  3. A gardener removes a complete ring of bark from a tree trunk. Explain why the tree eventually dies. [3]3 marks

Key terms

TermMeaning
TranslocationThe transport of dissolved sugars in the phloem.
SourceA part of the plant that makes or releases sugars.
SinkA part of the plant that uses or stores sugars.
Sieve plateA perforated end wall between phloem sieve tube elements.
Companion cellA cell next to a sieve tube that provides energy and carries out its metabolism.
SucroseThe sugar in which food is transported through the phloem.
Mass flowThe movement of fluid along a pressure gradient.

Avoid these mistakes

1
Saying phloem carries glucoseit carries sucrose.
2
Writing that translocation only moves upwardsit moves from sources to sinks in either direction.
3
Claiming that both transport systems use dead cells: phloem cells are alive.
4
Saying a leaf is always a source and a root always a sink: the roles depend on the stage of the plant.

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