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2.5Structures and Functions in Living Organisms

Nutrition — Edexcel Linear - OL Revision Notes & Topical Questions

20 min read 28 parts Lesson 20 of 25 Unit quiz

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Photosynthesis and the Factors that Limit its Rate — EDEXCEL LINEAR - OL notes: Write the equation for photosynthesis, describe the uses of glucose and interpret how temperature, light and carbon dioxide limit the rate.Photosynthesis is the process in which energy from sunlight i…

  • chlorophyll
  • carbon dioxide and water
  • limiting factor
  • Worked example 1
  • Worked example 2
  • Worked example 3

Section 1 of 28The process of photosynthesis

Photosynthesis is the process in which energy from sunlight is transferred to the chloroplasts of green plants.

1Chlorophyll
The light is absorbed by chlorophyll, the green pigment in chloroplasts.
2Carbon dioxide and water
Green plants use this energy to make the carbohydrate glucose from the raw materials carbon dioxide and water,
  • and oxygen is released as a waste product.
3
Photosynthesis can be defined as the process by which plants manufacture carbohydrates from raw materials using energy from light.
4
Plants are producers, the first organisms in all food chains.
5
If asked for the raw materials, the answer is carbon dioxide and water; light is energy, not a substance.

Word equation

  • carbon dioxide + water → glucose + oxygen (in light, with chlorophyll).
  • Symbol equation: 6CO2 + 6H2O → C6H12O6 + 6O2.
  • This is the exact reverse of aerobic respiration, so learning one gives you the other.

Section 2 of 28Uses of the glucose made

Plants use glucose as a source of energy in respiration.

Uses of the glucose made
They can also use it to produce starch for storage, to synthesise lipids as an energy source in seeds, to form cellulose for cell walls,
  • and to produce amino acids (for proteins) when combined with nitrogen and other mineral ions absorbed by the roots.
Once the carbon from carbon dioxide has been converted into glucose it can be used to build many biological molecules.

Section 3 of 28Limiting factors

A limiting factor is something present in the environment in such short supply that it restricts a life process; the rate of photosynthesis is limited by whichever factor is lowest.

Limiting factors
The three main factors are temperature, light intensity and carbon dioxide concentration.
Water is not considered a limiting factor because the amount needed is small compared with the amount lost by transpiration.
The number of chloroplasts or the amount of chlorophyll also affects the rate.
FactorEffect on the rate
TemperatureRaises kinetic energy and collisions between reactants and enzymes, so the rate increases up to the optimum; above it the enzymes are denatured and the rate falls
Light intensityMore light gives a faster rate until another factor becomes limiting
Carbon dioxideA raw material; more gives a faster rate until another factor becomes limiting
Chlorophyll / chloroplastsMore means faster; reduced by disease (tobacco mosaic virus), lack of magnesium, loss of leaves and variegated leaves

Section 4 of 28Reading limiting factor graphs

Where the line is rising, the limiting factor is the one on the x-axis.

Reading limiting factor graphs
Where the line is horizontal, the limiting factor is something else: temperature, light intensity or carbon dioxide concentration (not the one on the axis).
For example, on a light intensity graph, the plateau means light is no longer limiting and carbon dioxide or temperature is.
Describe the graph then explain it.

Section 5 of 28Photosynthesis and respiration compared

FeaturePhotosynthesisAerobic respiration
WhereChloroplastsMitochondria (and cytoplasm)
EnergyAbsorbs light energyReleases energy
ReactantsCarbon dioxide and waterGlucose and oxygen
ProductsGlucose and oxygenCarbon dioxide and water
WhenIn lightAll the time

Photosynthesis therefore takes carbon dioxide out of the atmosphere and adds oxygen, whereas respiration does the reverse, and the two processes keep the atmosphere balanced.

Section 6 of 28Worked examples

  1. State the word equation for photosynthesis. [2]2 marks

  2. Explain why the rate of photosynthesis levels off as light intensity increases. [3]3 marks

  3. Why does the rate fall above about 45 °C? [2]2 marks

  4. Name two uses of glucose in a plant. [2]2 marks

  5. Explain why water is rarely a limiting factor. [1]1 mark

Section 7 of 28Structure of a leaf

Leaves have complex structures with layers of different tissues containing specially adapted cells.

StructureDescription and function
Wax cuticleProtective layer on upper and lower sides; prevents water loss
Upper epidermisThin and transparent to allow light to enter the palisade layer
Palisade mesophyllColumn-shaped cells tightly packed with chloroplasts to absorb more light
Spongy mesophyllInternal air spaces increase the surface area for diffusion of gases (mainly carbon dioxide)
Lower epidermisContains guard cells and stomata
Guard cellsAbsorb and lose water to open and close the stomata
StomataWhere gas exchange occurs; open in the day, closed at night; most are on the underside to reduce water loss
Vascular bundleContains xylem (brings water) and phloem (transports sucrose and amino acids)

Leaves are adapted for photosynthesis by having a large surface area, a palisade layer near the top that is hit directly by light, and an air-space-filled spongy layer.

Section 8 of 28Plants and mineral ions

Plants must make proteins, lipids and nucleic acids themselves because they do not eat. Carbohydrates contain C, H and O, but proteins also contain nitrogen. Two fundamental mineral ions are nitrate (for amino acids and proteins) and magnesium (for chlorophyll). A lack of nitrate leads to stunted growth; a lack of magnesium causes yellow leaves (chlorosis) and poor photosynthesis.

Section 9 of 28Practical: oxygen release and the need for light

To show that oxygen is released, use a water plant such as Elodea in a beaker of water. Oxygen produced as bubbles leaves the cut end of the stem; counting the bubbles per minute estimates the rate. To show the need for light:

1
De-starch the plant by placing it in a dark cupboard for 24 hours.
2
Cover part of one leaf with aluminium foil and place the plant in bright light for several hours.
3
Dip the leaf in boiling water, then heat it in ethanol (in a water bath, with no flames) to remove chlorophyll.
4
Rinse, spread on a white tile and cover with iodine solution.

Glucose is not tested because it is quickly converted to other substances or stored as starch; starch is stored in chloroplasts and is a reliable indicator of photosynthesis.

Practical: oxygen release and the need for light
The uncovered area turns blue-black; the covered area stays orange-brown, which shows that light is needed for photosynthesis.
When the cells cannot photosynthesise they break down starch to use the glucose for respiration.

Section 10 of 28Mineral deficiency and other leaf features

To compare deficiencies

  • a plant lacking nitrate has stunted growth and yellow older leaves, because nitrate is moved to new growth;
    • a plant lacking magnesium has yellow leaves because it cannot make chlorophyll.

1
Both reduce photosynthesis and yield.
2
Roots take in these ions by active transport, so waterlogged soil, which lacks oxygen, reduces mineral uptake.
3
The leaf’s flat shape and thinness reduce the distance gases and light must travel, and the veins support the leaf and bring water.
4
The thin cuticle on the upper surface lets light through while still limiting water loss.

Remember to name the correct tissue when explaining transport: xylem delivers the water and mineral ions the leaf needs, and phloem carries away the sucrose made, so photosynthesis in the mesophyll depends on both.

Section 11 of 28Worked examples

  1. Explain how the palisade layer is adapted for photosynthesis. [3]3 marks

  2. State the function of guard cells. [1]1 mark

  3. Which mineral ion is needed to make proteins? [1]1 mark

  4. Why is the leaf boiled in ethanol? [2]2 marks

  5. Why is a plant destarched first? [1]1 mark

Section 12 of 28Practical: is carbon dioxide needed for photosynthesis?

1
De-starch the plant in a dark cupboard for 24 hours so existing starch is used up and cannot affect the results.
2
Enclose one leaf in a conical flask containing potassium hydroxide, which absorbs carbon dioxide, sealed with a bung.
3
Enclose another leaf in a control flask not containing potassium hydroxide.
4
Leave the plant in bright light for several hours.
5
Remove the leaves, dip in boiling water, and heat in ethanol in an electric water bath to remove chlorophyll.
6
Spread each leaf out on a white tile and cover with iodine solution.

The leaf from the potassium hydroxide flask stays orange-brown because it could not photosynthesise without carbon dioxide; the control leaf turns blue-black because it had everything needed.

Safety

  • ethanol is extremely flammable, so turn the Bunsen burner off at that stage and heat the ethanol in an electric water bath, not a beaker over a flame.
  • Wear eye protection;
    • potassium hydroxide is corrosive.

Section 13 of 28Applying CORMS

LetterIn this practical
C – ChangeWhether there is carbon dioxide or no carbon dioxide
O – OrganismLeaves from the same plant or species, of the same age and size
R – RepeatRepeat several times to ensure reliable results
M – MeasureObserve the colour change of the leaf when iodine is applied
S – SameControl the temperature of the room and the light intensity

CORMS questions will probably ask about unfamiliar experiments, so practise applying CORMS to many different practical scenarios.

Section 14 of 28A balanced diet

A balanced diet contains all the food groups in the correct proportions. An unbalanced diet can lead to malnutrition, which causes many health problems.

Food groupSourcesFunction
CarbohydratesBread, cereals, pasta, rice, potatoesMain energy source
ProteinsMeat, fish, eggs, beansGrowth and repair
LipidsButter, oils, nutsEnergy store, insulation
FibreVegetables, whole grainsProvides bulk (roughage) for the intestine to push food through
VitaminsFruits and vegetables, meats, dairy productsNeeded in small quantities to maintain health (e.g. vitamin C prevents scurvy, vitamin D helps bones)
MineralsDairy, leafy vegetablesCalcium for bones and teeth; iron for haemoglobin
WaterDrinks and foodSolvent for reactions and transport

The amount of energy needed depends on age, activity and sex; young people and active people need more. For example, teenagers and pregnant women need more energy, and a manual worker needs more than an office worker.

Section 15 of 28Interpreting results and drawing conclusions

In the carbon dioxide experiment the conclusion depends on comparing the two leaves: if both leaves turned blue-black, carbon dioxide may still have entered the flask, so the seal must be airtight.

Interpreting results and drawing conclusions
The control leaf proves the plant could photosynthesise and the leaf in potassium hydroxide proves that it cannot without carbon dioxide.
A good conclusion states: “Carbon dioxide is needed for photosynthesis because the leaf without carbon dioxide contained no starch.” You should also evaluate: results from one leaf only are not reliable,
  • so repeat with several leaves.
For diet questions, always link the nutrient to its function and to a deficiency effect.

Hint for examswhen asked to name a variable that was controlled, give a specific one such as the same plant, the same light intensity and the same time left in the light, rather than just saying “the conditions”.

Section 16 of 28Worked examples

  1. Explain the purpose of potassium hydroxide in the flask. [2]2 marks

  2. Why is a control flask used? [1]1 mark

  3. State two safety precautions when using ethanol. [2]2 marks

  4. Name the deficiency disease caused by a lack of vitamin C. [1]1 mark

  5. Why is fibre needed in the diet? [1]1 mark

Section 17 of 28The digestive system and the alimentary canal

The digestive system is an example of an organ system. It includes the organs of the alimentary canal (the tube food passes through) and the accessory organs (liver, gall bladder and pancreas) that work together to break large insoluble molecules into small soluble ones that can be absorbed.

OrganRole in digestion
MouthTeeth chew (mechanical digestion); saliva contains amylase; tongue forms a bolus
OesophagusCarries the bolus to the stomach by peristalsis
StomachFood is mechanically digested by churning; protease enzymes begin protein digestion; hydrochloric acid kills bacteria and provides the optimum pH for protease
Small intestineDuodenum: digestion completed by enzymes from the pancreas and the lining; the pH is slightly alkaline, about 8–9; ileum: absorption
Large intestineAbsorbs water
Liver, gall bladder, pancreasAccessory organs: bile made and stored; the pancreas makes enzymes and insulin

Section 18 of 28Peristalsis

Peristalsis is a mechanism that moves food along the alimentary canal. It is controlled by circular and longitudinal muscles in the gut wall.

1
Muscles in the oesophagus wall create waves of contractions that force the bolus along.
2
Circular muscles contract behind the bolus, narrowing the tube, while longitudinal muscles contract to shorten and push the bolus onward.
3
The bolus reaches the stomach, where it is churned into a less solid form called chyme.
4
Chyme passes on to the small intestine, and the waves continue through the intestines.

Section 19 of 28Digestive enzymes

The purpose of digestion is to break large, insoluble molecules into smaller, soluble molecules that can be absorbed into the bloodstream. Food is partly digested mechanically (chewing, churning, emulsification), which increases surface area, but digestion mainly takes place chemically: bonds holding the large molecules together are broken by enzymes made in different parts of the system.

TypeExamplesSubstrate → products
CarbohydrasesAmylase, maltaseStarch → maltose → glucose
ProteasesPepsin (stomach), proteases from pancreas and small intestineProtein → polypeptides → amino acids
LipasesPancreatic and intestinal lipaseLipids → glycerol and fatty acids

Pepsin is made in the stomach and breaks proteins into smaller polypeptide chains; proteases made in the pancreas and small intestine break these chains into amino acids. The pancreas is an accessory organ: food does not pass through it, but it releases digestive enzymes into the small intestine and makes the hormone insulin.

Section 20 of 28Bile production and storage

Bile is an alkaline substance produced by cells in the liver and stored in the gall bladder before being released into the small intestine. It neutralises the acid from the stomach and its bile salts emulsify lipids, as described in the next lesson.

Section 21 of 28Linking structure to function in the gut

The wall of the alimentary canal has layers of muscle, glands and a lining that suits each region.

1
The stomach wall is muscular and has glands secreting acid and pepsin; the small intestine has glands and a lining with villi; the large intestine has a smooth lining to absorb water.
2
The long length of the intestines gives time for digestion and absorption: the small intestine is about six metres in an adult.
3
When asked to explain digestion, name the organ, the enzyme, the substrate and the product, for example: amylase from the salivary glands and pancreas digests starch to maltose.
4
Remember that chemical digestion needs specific enzymes at their optimum pH, which is why the acid stomach and alkaline duodenum are so different.

Section 22 of 28Worked examples

  1. Describe how peristalsis moves food. [3]3 marks

  2. State the products of protein digestion. [1]1 mark

  3. Explain why the stomach contains hydrochloric acid. [2]2 marks

  4. Name the enzymes that convert starch to glucose and the intermediate. [3]3 marks

  5. Why is the pancreas called an accessory organ? [1]1 mark

Section 23 of 28Bile and emulsification

Bile has two roles.

First it neutralises the hydrochloric acid arriving from the stomach, creating the alkaline conditions in which pancreatic and intestinal enzymes work best.
Emulsification
Second, it breaks apart large drops of lipid into smaller droplets, which increases their surface area: this is emulsification.
The more alkaline conditions and larger surface area let lipase chemically break lipids down into glycerol and fatty acids at a faster rate.
Bile salts do the emulsifying.

Emulsification is like tearing a large piece of paper into smaller pieces: it is mechanical digestion, not chemical, because breaking something into smaller pieces does not break bonds or change the chemical structure of the molecules.

Section 24 of 28Absorption and the adaptations of the small intestine

Absorption is the movement of small digested molecules from the digestive system into the blood (glucose and amino acids) and lymph (fatty acids and glycerol). Small soluble molecules move by diffusion and sometimes active transport; water is absorbed by osmosis, mainly in the small intestine but also in the large intestine.

AdaptationHow it helps absorption
Very long, folded small intestineLarge surface area and more time for absorption
Villi (finger-like projections) with microvilliGreatly increase the surface area
Thin wall, one cell thickShort diffusion distance
Rich network of capillariesRemoves absorbed molecules and maintains a steep concentration gradient
Lacteal (lymph vessel)Takes up fatty acids and glycerol
Many mitochondriaSupply energy for active transport of glucose

The structure of a villus in relation to its function is a frequent exam question, so learn these adaptations and how each affects the rate of absorption.

Section 25 of 28Practical: energy content of a food sample

Burn food samples to compare energy contents using simple calorimetry.

1
Measure 25 cm3 of water into a boiling tube and record its temperature.
2
Weigh a small sample of food on a mounted needle.
3
Light the food in a Bunsen flame and hold it under the tube until it stops burning.
4
Record the final temperature, stir first, and calculate the temperature rise.

The larger the rise in water temperature, the more energy the food stored.

Practical: energy content of a food sample
Energy transferred (J) = mass of water (g) × temperature rise (°C) × 4.2, and energy per gram = energy ÷ mass of food (g).
Example: 25 g of water rising by 16 °C when 0.5 g burns gives 25 × 16 × 4.2 = 1680 J, so 3360 J/g.

Sources of error

  • some food may not burn completely, so not all its energy is released (solution: relight the sample until it will not light);
    • heat is lost to the surroundings (use a shield and insulation, and keep the tube close to the flame).
  • The measured value is lower than the true one.

Section 26 of 28Worked examples

  1. State two functions of bile. [2]2 marks

  2. Explain why emulsification speeds up lipid digestion. [2]2 marks

  3. Describe three adaptations of a villus. [3]3 marks

  4. A sample of 0.8 g heats 30 g of water by 20 °C. Calculate the energy per gram. [3]3 marks

  5. Why is the experimental energy value too low? [2]2 marks

Section 27 of 28Key terms

TermMeaning
PhotosynthesisProcess by which plants make glucose from CO2 and water using light energy.
ChlorophyllGreen pigment in chloroplasts that absorbs light.
Limiting factorA factor in short supply that restricts a process.
ProducerAn organism that makes its own food, forming the start of a food chain.
Raw materialA substance used up in a reaction.
TermMeaning
StomataPores in the leaf epidermis for gas exchange.
Guard cellA cell that opens and closes a stoma.
Palisade mesophyllLeaf tissue with many chloroplasts that performs most photosynthesis.
CuticleWaxy layer that reduces water loss.
XylemTissue that transports water and mineral ions.
PhloemTissue that transports sucrose and amino acids.
TermMeaning
Potassium hydroxideA chemical that absorbs carbon dioxide.
ControlThe part of an experiment used for comparison.
MalnutritionPoor health from a diet lacking a balance of nutrients.
FibrePlant material that adds bulk to food in the intestine.
Balanced dietA diet with all food groups in the right amounts.
TermMeaning
Alimentary canalThe tube from mouth to anus through which food passes.
PeristalsisWaves of muscular contraction that move food.
ChymeSemi-liquid mixture of food and gastric juice.
CarbohydraseAn enzyme that digests carbohydrates.
ProteaseAn enzyme that digests proteins.
LipaseAn enzyme that digests lipids.
TermMeaning
EmulsificationBreaking lipid into tiny droplets, increasing surface area.
VillusFinger-like projection of the small intestine.
LactealA lymph vessel inside a villus.
AbsorptionMovement of digested food into the blood or lymph.
CalorimetryMeasuring energy by heating water.

Section 28 of 28Exam technique

1
Listing light as a raw material.
2
Saying plants photosynthesise only in the day and respire only at night.
3
Saying temperature always increases the rate — enzymes are denatured at high temperatures.
4
Reading the wrong factor as limiting on the flat part of a graph.
1
Saying stomata are mostly on the upper surface.
2
Saying magnesium is needed to make protein.
3
Testing for glucose rather than starch.
4
Heating ethanol with a naked flame.
1
Forgetting to destarch before the experiment.
2
Heating ethanol with a Bunsen flame.
3
Thinking fibre provides energy.
4
Using only one leaf with no control.
1
Saying digestion happens in the large intestine.
2
Saying peristalsis is caused by gravity.
3
Saying all digestion is chemical — chewing and churning are mechanical.
4
Forgetting that enzymes are specific to one substrate.
1
Saying bile contains enzymes.
2
Saying emulsification is chemical digestion.
3
Forgetting that fatty acids and glycerol enter the lacteal (lymph).
4
Not multiplying by 4.2 in the energy calculation.

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