2.5Structures and Functions in Living Organisms
Nutrition — Edexcel Linear - OL Revision Notes & Topical Questions
20 min read 28 parts Lesson 20 of 25 Unit quiz
Photosynthesis and the Factors that Limit its Rate — EDEXCEL LINEAR - OL notes: Write the equation for photosynthesis, describe the uses of glucose and interpret how temperature, light and carbon dioxide limit the rate.Photosynthesis is the process in which energy from sunlight i…
- chlorophyll
- carbon dioxide and water
- limiting factor
- Worked example 1
- Worked example 2
- Worked example 3
Section 1 of 28The process of photosynthesis
Photosynthesis is the process in which energy from sunlight is transferred to the chloroplasts of green plants.
- and oxygen is released as a waste product.
Word equation
- carbon dioxide + water → glucose + oxygen (in light, with chlorophyll).
- Symbol equation: 6CO2 + 6H2O → C6H12O6 + 6O2.
- This is the exact reverse of aerobic respiration, so learning one gives you the other.
Section 2 of 28Uses of the glucose made
Plants use glucose as a source of energy in respiration.
- and to produce amino acids (for proteins) when combined with nitrogen and other mineral ions absorbed by the roots.
Section 3 of 28Limiting factors
A limiting factor is something present in the environment in such short supply that it restricts a life process; the rate of photosynthesis is limited by whichever factor is lowest.
| Factor | Effect on the rate |
|---|---|
| Temperature | Raises kinetic energy and collisions between reactants and enzymes, so the rate increases up to the optimum; above it the enzymes are denatured and the rate falls |
| Light intensity | More light gives a faster rate until another factor becomes limiting |
| Carbon dioxide | A raw material; more gives a faster rate until another factor becomes limiting |
| Chlorophyll / chloroplasts | More means faster; reduced by disease (tobacco mosaic virus), lack of magnesium, loss of leaves and variegated leaves |
Section 4 of 28Reading limiting factor graphs
Where the line is rising, the limiting factor is the one on the x-axis.
Section 5 of 28Photosynthesis and respiration compared
| Feature | Photosynthesis | Aerobic respiration |
|---|---|---|
| Where | Chloroplasts | Mitochondria (and cytoplasm) |
| Energy | Absorbs light energy | Releases energy |
| Reactants | Carbon dioxide and water | Glucose and oxygen |
| Products | Glucose and oxygen | Carbon dioxide and water |
| When | In light | All the time |
Photosynthesis therefore takes carbon dioxide out of the atmosphere and adds oxygen, whereas respiration does the reverse, and the two processes keep the atmosphere balanced.
Section 6 of 28Worked examples
State the word equation for photosynthesis. [2]2 marks
Carbon dioxide + water → glucose + oxygen (with light energy and chlorophyll).
Explain why the rate of photosynthesis levels off as light intensity increases. [3]3 marks
Another factor, such as carbon dioxide concentration or temperature, becomes limiting, so extra light cannot increase the rate.
Why does the rate fall above about 45 °C? [2]2 marks
The enzymes controlling photosynthesis are denatured.
Name two uses of glucose in a plant. [2]2 marks
Respiration for energy and making starch for storage (or cellulose, lipids, amino acids).
Explain why water is rarely a limiting factor. [1]1 mark
The amount needed is small compared with the amount transpired.
Section 7 of 28Structure of a leaf
Leaves have complex structures with layers of different tissues containing specially adapted cells.
| Structure | Description and function |
|---|---|
| Wax cuticle | Protective layer on upper and lower sides; prevents water loss |
| Upper epidermis | Thin and transparent to allow light to enter the palisade layer |
| Palisade mesophyll | Column-shaped cells tightly packed with chloroplasts to absorb more light |
| Spongy mesophyll | Internal air spaces increase the surface area for diffusion of gases (mainly carbon dioxide) |
| Lower epidermis | Contains guard cells and stomata |
| Guard cells | Absorb and lose water to open and close the stomata |
| Stomata | Where gas exchange occurs; open in the day, closed at night; most are on the underside to reduce water loss |
| Vascular bundle | Contains xylem (brings water) and phloem (transports sucrose and amino acids) |
Leaves are adapted for photosynthesis by having a large surface area, a palisade layer near the top that is hit directly by light, and an air-space-filled spongy layer.
Section 8 of 28Plants and mineral ions
Plants must make proteins, lipids and nucleic acids themselves because they do not eat. Carbohydrates contain C, H and O, but proteins also contain nitrogen. Two fundamental mineral ions are nitrate (for amino acids and proteins) and magnesium (for chlorophyll). A lack of nitrate leads to stunted growth; a lack of magnesium causes yellow leaves (chlorosis) and poor photosynthesis.
Section 9 of 28Practical: oxygen release and the need for light
To show that oxygen is released, use a water plant such as Elodea in a beaker of water. Oxygen produced as bubbles leaves the cut end of the stem; counting the bubbles per minute estimates the rate. To show the need for light:
Glucose is not tested because it is quickly converted to other substances or stored as starch; starch is stored in chloroplasts and is a reliable indicator of photosynthesis.
Section 10 of 28Mineral deficiency and other leaf features
To compare deficiencies
- a plant lacking nitrate has stunted growth and yellow older leaves, because nitrate is moved to new growth;
- a plant lacking magnesium has yellow leaves because it cannot make chlorophyll.
Remember to name the correct tissue when explaining transport: xylem delivers the water and mineral ions the leaf needs, and phloem carries away the sucrose made, so photosynthesis in the mesophyll depends on both.
Section 11 of 28Worked examples
Explain how the palisade layer is adapted for photosynthesis. [3]3 marks
Cells are column-shaped and tightly packed under the upper epidermis, with many chloroplasts to absorb light.
State the function of guard cells. [1]1 mark
To open and close the stomata by gaining and losing water.
Which mineral ion is needed to make proteins? [1]1 mark
Nitrate.
Why is the leaf boiled in ethanol? [2]2 marks
To remove chlorophyll so that the colour change with iodine can be seen.
Why is a plant destarched first? [1]1 mark
To make sure that any starch found was made during the experiment.
Section 12 of 28Practical: is carbon dioxide needed for photosynthesis?
The leaf from the potassium hydroxide flask stays orange-brown because it could not photosynthesise without carbon dioxide; the control leaf turns blue-black because it had everything needed.
Safety
- ethanol is extremely flammable, so turn the Bunsen burner off at that stage and heat the ethanol in an electric water bath, not a beaker over a flame.
- Wear eye protection;
- potassium hydroxide is corrosive.
Section 13 of 28Applying CORMS
| Letter | In this practical |
|---|---|
| C – Change | Whether there is carbon dioxide or no carbon dioxide |
| O – Organism | Leaves from the same plant or species, of the same age and size |
| R – Repeat | Repeat several times to ensure reliable results |
| M – Measure | Observe the colour change of the leaf when iodine is applied |
| S – Same | Control the temperature of the room and the light intensity |
CORMS questions will probably ask about unfamiliar experiments, so practise applying CORMS to many different practical scenarios.
Section 14 of 28A balanced diet
A balanced diet contains all the food groups in the correct proportions. An unbalanced diet can lead to malnutrition, which causes many health problems.
| Food group | Sources | Function |
|---|---|---|
| Carbohydrates | Bread, cereals, pasta, rice, potatoes | Main energy source |
| Proteins | Meat, fish, eggs, beans | Growth and repair |
| Lipids | Butter, oils, nuts | Energy store, insulation |
| Fibre | Vegetables, whole grains | Provides bulk (roughage) for the intestine to push food through |
| Vitamins | Fruits and vegetables, meats, dairy products | Needed in small quantities to maintain health (e.g. vitamin C prevents scurvy, vitamin D helps bones) |
| Minerals | Dairy, leafy vegetables | Calcium for bones and teeth; iron for haemoglobin |
| Water | Drinks and food | Solvent for reactions and transport |
The amount of energy needed depends on age, activity and sex; young people and active people need more. For example, teenagers and pregnant women need more energy, and a manual worker needs more than an office worker.
Section 15 of 28Interpreting results and drawing conclusions
In the carbon dioxide experiment the conclusion depends on comparing the two leaves: if both leaves turned blue-black, carbon dioxide may still have entered the flask, so the seal must be airtight.
- so repeat with several leaves.
Hint for examswhen asked to name a variable that was controlled, give a specific one such as the same plant, the same light intensity and the same time left in the light, rather than just saying “the conditions”.
Section 16 of 28Worked examples
Explain the purpose of potassium hydroxide in the flask. [2]2 marks
It absorbs carbon dioxide so the leaf cannot photosynthesise.
Why is a control flask used? [1]1 mark
To show what happens when carbon dioxide is present.
State two safety precautions when using ethanol. [2]2 marks
Turn off flames and heat it in an electric water bath.
Name the deficiency disease caused by a lack of vitamin C. [1]1 mark
Scurvy.
Why is fibre needed in the diet? [1]1 mark
It provides bulk to help push food through the intestine.
Section 17 of 28The digestive system and the alimentary canal
The digestive system is an example of an organ system. It includes the organs of the alimentary canal (the tube food passes through) and the accessory organs (liver, gall bladder and pancreas) that work together to break large insoluble molecules into small soluble ones that can be absorbed.
| Organ | Role in digestion |
|---|---|
| Mouth | Teeth chew (mechanical digestion); saliva contains amylase; tongue forms a bolus |
| Oesophagus | Carries the bolus to the stomach by peristalsis |
| Stomach | Food is mechanically digested by churning; protease enzymes begin protein digestion; hydrochloric acid kills bacteria and provides the optimum pH for protease |
| Small intestine | Duodenum: digestion completed by enzymes from the pancreas and the lining; the pH is slightly alkaline, about 8–9; ileum: absorption |
| Large intestine | Absorbs water |
| Liver, gall bladder, pancreas | Accessory organs: bile made and stored; the pancreas makes enzymes and insulin |
Section 18 of 28Peristalsis
Peristalsis is a mechanism that moves food along the alimentary canal. It is controlled by circular and longitudinal muscles in the gut wall.
Section 19 of 28Digestive enzymes
The purpose of digestion is to break large, insoluble molecules into smaller, soluble molecules that can be absorbed into the bloodstream. Food is partly digested mechanically (chewing, churning, emulsification), which increases surface area, but digestion mainly takes place chemically: bonds holding the large molecules together are broken by enzymes made in different parts of the system.
| Type | Examples | Substrate → products |
|---|---|---|
| Carbohydrases | Amylase, maltase | Starch → maltose → glucose |
| Proteases | Pepsin (stomach), proteases from pancreas and small intestine | Protein → polypeptides → amino acids |
| Lipases | Pancreatic and intestinal lipase | Lipids → glycerol and fatty acids |
Pepsin is made in the stomach and breaks proteins into smaller polypeptide chains; proteases made in the pancreas and small intestine break these chains into amino acids. The pancreas is an accessory organ: food does not pass through it, but it releases digestive enzymes into the small intestine and makes the hormone insulin.
Section 20 of 28Bile production and storage
Bile is an alkaline substance produced by cells in the liver and stored in the gall bladder before being released into the small intestine. It neutralises the acid from the stomach and its bile salts emulsify lipids, as described in the next lesson.
Section 21 of 28Linking structure to function in the gut
The wall of the alimentary canal has layers of muscle, glands and a lining that suits each region.
Section 22 of 28Worked examples
Describe how peristalsis moves food. [3]3 marks
Waves of contraction of the circular and longitudinal muscles in the gut wall squeeze the food along the canal.
State the products of protein digestion. [1]1 mark
Amino acids.
Explain why the stomach contains hydrochloric acid. [2]2 marks
It kills bacteria in food and gives the acid pH at which pepsin works best.
Name the enzymes that convert starch to glucose and the intermediate. [3]3 marks
Amylase converts starch to maltose; maltase converts maltose to glucose.
Why is the pancreas called an accessory organ? [1]1 mark
Food does not pass through it, but it makes digestive enzymes and insulin.
Section 23 of 28Bile and emulsification
Bile has two roles.
Emulsification is like tearing a large piece of paper into smaller pieces: it is mechanical digestion, not chemical, because breaking something into smaller pieces does not break bonds or change the chemical structure of the molecules.
Section 24 of 28Absorption and the adaptations of the small intestine
Absorption is the movement of small digested molecules from the digestive system into the blood (glucose and amino acids) and lymph (fatty acids and glycerol). Small soluble molecules move by diffusion and sometimes active transport; water is absorbed by osmosis, mainly in the small intestine but also in the large intestine.
| Adaptation | How it helps absorption |
|---|---|
| Very long, folded small intestine | Large surface area and more time for absorption |
| Villi (finger-like projections) with microvilli | Greatly increase the surface area |
| Thin wall, one cell thick | Short diffusion distance |
| Rich network of capillaries | Removes absorbed molecules and maintains a steep concentration gradient |
| Lacteal (lymph vessel) | Takes up fatty acids and glycerol |
| Many mitochondria | Supply energy for active transport of glucose |
The structure of a villus in relation to its function is a frequent exam question, so learn these adaptations and how each affects the rate of absorption.
Section 25 of 28Practical: energy content of a food sample
Burn food samples to compare energy contents using simple calorimetry.
The larger the rise in water temperature, the more energy the food stored.
Sources of error
- some food may not burn completely, so not all its energy is released (solution: relight the sample until it will not light);
- heat is lost to the surroundings (use a shield and insulation, and keep the tube close to the flame).
- The measured value is lower than the true one.
Section 26 of 28Worked examples
State two functions of bile. [2]2 marks
It neutralises stomach acid and emulsifies lipids.
Explain why emulsification speeds up lipid digestion. [2]2 marks
It breaks lipid drops into droplets with a larger surface area for lipase to act on.
Describe three adaptations of a villus. [3]3 marks
A large surface area, thin walls and a good capillary supply.
A sample of 0.8 g heats 30 g of water by 20 °C. Calculate the energy per gram. [3]3 marks
30 × 20 × 4.2 = 2520 J; 2520 ÷ 0.8 = 3150 J/g.
Why is the experimental energy value too low? [2]2 marks
Heat is lost to the surroundings and burning is incomplete.
Section 27 of 28Key terms
| Term | Meaning |
|---|---|
| Photosynthesis | Process by which plants make glucose from CO2 and water using light energy. |
| Chlorophyll | Green pigment in chloroplasts that absorbs light. |
| Limiting factor | A factor in short supply that restricts a process. |
| Producer | An organism that makes its own food, forming the start of a food chain. |
| Raw material | A substance used up in a reaction. |
| Term | Meaning |
|---|---|
| Stomata | Pores in the leaf epidermis for gas exchange. |
| Guard cell | A cell that opens and closes a stoma. |
| Palisade mesophyll | Leaf tissue with many chloroplasts that performs most photosynthesis. |
| Cuticle | Waxy layer that reduces water loss. |
| Xylem | Tissue that transports water and mineral ions. |
| Phloem | Tissue that transports sucrose and amino acids. |
| Term | Meaning |
|---|---|
| Potassium hydroxide | A chemical that absorbs carbon dioxide. |
| Control | The part of an experiment used for comparison. |
| Malnutrition | Poor health from a diet lacking a balance of nutrients. |
| Fibre | Plant material that adds bulk to food in the intestine. |
| Balanced diet | A diet with all food groups in the right amounts. |
| Term | Meaning |
|---|---|
| Alimentary canal | The tube from mouth to anus through which food passes. |
| Peristalsis | Waves of muscular contraction that move food. |
| Chyme | Semi-liquid mixture of food and gastric juice. |
| Carbohydrase | An enzyme that digests carbohydrates. |
| Protease | An enzyme that digests proteins. |
| Lipase | An enzyme that digests lipids. |
| Term | Meaning |
|---|---|
| Emulsification | Breaking lipid into tiny droplets, increasing surface area. |
| Villus | Finger-like projection of the small intestine. |
| Lacteal | A lymph vessel inside a villus. |
| Absorption | Movement of digested food into the blood or lymph. |
| Calorimetry | Measuring energy by heating water. |
Section 28 of 28Exam technique
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