Skip to content
Dabour HubLEARN · PRACTICE · EXPLORESign inStudent

5.1Use of Biological Resources

Food production — Edexcel Modular - OL Revision Notes & Topical Questions

15 min read 19 parts Lesson 3 of 5 2 quizzes

📚 Edexcel Modular - OL Hub / Topical Revision
🩺
100% Curriculum Specification Match Lesson Doctor Verified
The only platform with 100% curriculum specification match and verified interactive question banks.
0%read
Lesson summary

Food production Edexcel O Level Modular Biology notes: describe how glasshouses and polythene tunnels can be used to increase the yield of certain cropsNot all microorganisms are harmful – many are useful.Yeast needs warmth, food and moisture to work.Too much heat kills yeast.des…

  • Food production Edexcel O Level Modular Biology: what to do next
  • Fermentation
  • Carbon dioxide
  • Lactic acid
  • Pasteurisation
  • Give / State / Name

Section 1 of 19Process & sequence

1
Mix flour, water, sugar and yeast.
2
Leave in a warm place.
3
Yeast ferments sugar → carbon dioxide.
4
Gas bubbles make the dough rise.
5
Baking kills the yeast and sets the bread.
  • Sugar
  • Yeast
  • Carbon dioxide
  • Dough rises

Section 2 of 19Keep going

Food production Edexcel O Level Modular Biology: what to do next

These Food production Edexcel O Level Modular Biology notes covered the key ideas, worked examples and exam technique for this topic. Check your Food production Edexcel O Level Modular Biology understanding with the practice questions below, then reread any Food production Edexcel O Level Modular Biology point you were unsure about before moving on.

Food production Edexcel O Level Modular Biology revision notes

Section 3 of 19Yeast and bread

Microorganisms can be used by humans to produce foods and other useful substances.

1
One example is the production of bread using yeast, a single-celled fungus that can carry out both aerobic and anaerobic respiration.
2
Yeast is mixed with flour and warm water and the dough is kept warm; the yeast respires, producing carbon dioxide that makes the dough rise.
3
The dough is then baked in a hot oven to form bread.
4
The yeast is killed by the high temperatures used during baking, which ensures there is no further respiration.

Section 4 of 19Practical: anaerobic respiration in yeast

Yeast can respire anaerobically (without oxygen), breaking down glucose to produce ethanol and carbon dioxide. Anaerobic respiration in yeast cells is called fermentation. Equation: glucose → ethanol + carbon dioxide.

1
Add yeast to a sugar solution; the sugar solution provides the yeast with glucose for anaerobic respiration.
2
Carefully add a layer of oil on top of the solution; this prevents oxygen entering and so prevents aerobic respiration.
3
Connect a delivery tube to limewater or a gas syringe to detect or measure the carbon dioxide produced.
4
Keep the tube in a water bath at a fixed temperature, and record bubbles per minute.
5
Repeat at several temperatures.

As respiration is an enzyme-controlled reaction, as enzyme activity increases with temperature the rate of carbon dioxide production increases.

Practical: anaerobic respiration in yeast
If the temperature is too high (beyond the optimum), the enzymes denature, causing carbon dioxide production to slow and eventually stop.
Remember CORMS: change temperature; organism - same yeast mass and sugar concentration; repeat; measure bubbles or gas volume; same time.
In your evaluation describe the trend, give data, explain using enzyme activity,
  • and suggest improvements.
    • (for example a gas syringe for more accurate volumes and a thermostatically controlled water bath)

Section 5 of 19Role of bacteria in yoghurt production

As well as fungi, bacteria are also used in the production of certain foods.

Role of bacteria in yoghurt production
Bacteria are useful because they are capable of producing complex molecules, for example certain bacteria added to milk produce enzymes that turn the milk into yoghurt.
The bacteria use lactose as their energy source and produce lactic acid,
  • which lowers the pH, causes the milk proteins to thicken, and gives yoghurt its sour taste.
The milk is first heated to kill unwanted microorganisms and then cooled to the optimum temperature for the bacteria, about 40 C.

Section 6 of 19Fermenters

Fermenters are containers used to grow microorganisms such as bacteria and fungi.

Fermenters
The microorganisms can then be used for making yoghurt and mycoprotein and other processes not involving food,
  • such as producing genetically modified bacteria and moulds that produce insulin and antibiotics.
The advantage is that conditions can be carefully controlled to produce large quantities of exactly the right type of microorganism.
ConditionControl
Optimum temperatureMonitored with probes and maintained using the water jacket; enzymes denature if the temperature is too high and work slowly if too low
Optimum pHMonitored with a probe, adjusted using acids or alkalis
OxygenationOxygen is needed for aerobic respiration, supplied by sterile air
AgitationStirring paddles ensure microorganisms, nutrients, oxygen, temperature and pH are evenly distributed

Section 7 of 19Comparing aerobic and anaerobic respiration in yeast

AerobicAnaerobic (fermentation)
OxygenNeededNot needed
ProductsCarbon dioxide and waterEthanol and carbon dioxide
Energy releasedMuch more per glucoseMuch less
UseRapid growth of yeastBread, beer, wine

In brewing the carbon dioxide escapes while ethanol builds up until the concentration is high enough to kill the yeast. Wine and beer production uses the same reaction, with yeast fermenting sugars from grapes or malted barley. Industrial fermenters are sterilised before use, because contaminating microbes would compete with the culture and could spoil the product.

Section 8 of 19Compare

Microorganisms in food production
FoodMicroorganismStarts withMicrobe producesEffect on foodConditions
BreadYeastFlour, sugar, waterCarbon dioxide + alcoholDough rises; alcohol evaporates on bakingWarm
YoghurtBacteria (Lactobacillus)MilkLactic acidMilk thickens, tastes sour~40 °C
CheeseBacteria (+ moulds)MilkLactic acidMilk solidifies into curdsControlled ripening
VinegarBacteriaWine / ciderEthanoic acidSour tasteWith oxygen
Soy sauceFungi + bacteriaSoybeans, wheatFlavour compoundsSalty savoury tasteMonths of fermentation

Section 9 of 19Worked examples

  1. Explain why bread dough rises. [3]3 marks

  2. Describe the effect on yeast respiration of raising the temperature from 20 C to 60 C. [3]3 marks

  3. Why is oil added to the sugar solution? [2]2 marks

  4. List two conditions controlled in a fermenter and say why. [4]4 marks

Section 10 of 19Crop plants in glasshouses and polythene tunnels

If a plant is given unlimited light, carbon dioxide and water at a warm temperature, the only thing limiting the rate of photosynthesis is its own ability to absorb these materials and make them react. Glasshouses and polythene tunnels let growers control these limiting factors to raise yield.

1Temperature
  • Temperature heaters or the trapped heat of the sun keep the enzymes of photosynthesis working at their optimum.
2Light
  • Light extra lighting extends the day length.
3Carbon dioxide
  • Carbon dioxide burners or gas supplies raise its concentration.
4Water
  • Water irrigation prevents shortage.
5Pests and diseases
  • Pests and diseases the enclosed space keeps most pests out.

The specification requires only that magnesium ions are needed for chlorophyll and nitrate ions for amino acids, so learn those two functions. Fertilisers supply them.

Section 11 of 19Pest control: pesticides and biological control

Another key way modern technology has increased food supply is the use of pesticides, which kill insects, weeds and fungi that reduce yield. Pesticides can harm other organisms and build up in food chains.

Biological control uses a natural predator or parasite to control a pest. This can happen naturally, for example ladybirds eat aphids. It leaves no chemical residues and is long lasting, but control is slower and the introduced species may become a pest itself.

Section 12 of 19Fish farming

Fish stocks in the wild are falling because of overfishing, so many fish are now farmed in ponds, cages or tanks.

Fish farming
This has several advantages over wild-caught fish,
  • including that stocks of wild fish are protected, the fish can be fed to grow quickly, the conditions are controlled,
    • (oxygen, temperature, disease control)
  • and predators and competitors are removed.
Disadvantages include the spread of disease and parasites, pollution from waste and food, and the use of antibiotics.

Section 13 of 19Genetic modification and biotechnology

Genetic modification (genetic engineering) is transferring a gene, or a piece of DNA, into another organism so that it gains a new characteristic. A student can remember the enzymes with a simple memory aid: a restriction enzyme is scissors (it cuts DNA at a specific sequence) and ligase is glue (it joins the cut pieces into one molecule).

TechniquePurposeKey steps / toolExample use
PCRCopy DNA95 °C denature, 55 °C anneal, 72 °C extend; Taq polymeraseForensics
Gel electrophoresisSeparate fragmentsLoad, run current, stain; agarose gelDNA fingerprinting
Restriction digestCut DNAMix DNA with restriction enzymeCloning (sticky ends)
Bacterial transformationInsert a geneHeat shock, select on antibiotic; plasmid and ligaseInsulin production
SequencingRead DNADideoxy / next-generation; DNA polymeraseGenome projects
CRISPREdit genesGuide RNA targets DNA; Cas9Gene therapy research

A transgenic organism contains DNA from a different species, so every transgenic organism is a GMO, but a GMO may have DNA from the same species.

Section 14 of 19Evaluating GM crops and microorganisms in food

AspectAdvantagesDisadvantages
ChemicalsFewer pesticides and herbicides neededDependence on particular herbicides
CostCheaper for farmers to growThe seeds themselves are expensive
YieldIncreased yieldSome GM crops grow less well
EnvironmentOverall benefit from reduced chemical useGene transfer to wild relatives can reduce biodiversity

Microorganisms such as yeast and bacteria can be used by humans to produce foods and other useful substances. In a yeast fermentation practical, carefully adding a layer of oil on top of the solution excludes oxygen, so the yeast respires anaerobically and no further aerobic respiration takes place.

When an exam says "compare", each point must refer to both items; when it says "explain", each marking point is usually a cause followed by a consequence, and unlinked facts often score nothing.

Section 15 of 19Worked examples

  1. Give two concerns people raise about growing GM crops. [2]2 marks

  2. Explain why a glasshouse increases crop yield. [3]3 marks

  3. State the roles of restriction enzymes and ligase in genetic modification. [2]2 marks

  4. Give two advantages of fish farming compared with catching wild fish. [2]2 marks

  5. Why is a layer of oil added on top of a yeast fermentation experiment? [1]1 mark

Section 16 of 19Key terms

TermMeaning
FermentationAnaerobic respiration in yeast making ethanol and CO2.
MycoproteinProtein food made from fungus.
AgitationStirring in a fermenter.
Optimum temperatureTemperature at which enzymes work fastest.
Lactic acidAcid made by yoghurt bacteria.
TermMeaning
Limiting factorA factor in short supply that restricts the rate of a process such as photosynthesis.
Biological controlUsing a living organism, such as a predator, to control a pest.
Genetic modificationTransferring a gene into another organism so it gains a new characteristic.
Restriction enzymeAn enzyme that cuts DNA at a specific base sequence.
LigaseAn enzyme that joins pieces of DNA.
Transgenic organismAn organism containing DNA from a different species.
PesticideA chemical that kills pests.

Section 17 of 19Syllabus checklist

1
describe how glasshouses and polythene tunnels can be used to increase the yield of certain crops
2
understand the effects on crop yield of increased carbon dioxide and increased temperature in glasshouses
3
understand how the use of fertiliser can increase crop yield
4
understand the reasons for pest control and the advantages and disadvantages of using pesticides and biological control with crop plants
5
understand the role of yeast in the production of food including bread
6
practical: investigate the role of anaerobic respiration by yeast in different conditions
7
understand the role of bacteria (Lactobacillus) in the production of yoghurt
8
understand the use of an industrial fermenter and explain the need to provide suitable conditions in the fermenter, including aseptic precautions, nutrients, optimum temperature and pH, oxygenation and agitation, for the growth of micro-organisms
9
understand the methods used to farm large numbers of fish to provide a source of protein,
  • including maintaining water quality, controlling intraspecific and interspecific predation, controlling disease, removing waste products, controlling the quality and frequency of feeding,
  • and selective breeding

Section 18 of 19Command terms

Command terms
Give / State / Name
Recall a fact; one idea per mark.
Define
Give the exact meaning using the specification keywords.
Describe
Give an account of what happens / the pattern in data, quoting values; no reasons needed.
Explain
Give reasons with linked statements (cause → effect). Each linked step is usually a separate marking point.
Suggest
Use knowledge in a new context; more than one answer may be accepted.
Compare
Similarities and differences; each point must refer to both items.
Calculate
Show working; include units; check significant figures. Method marks are given even if the final answer is wrong.
Discuss
Explore the issue from different angles and link the points.
Evaluate / Assess
Weigh strengths and weaknesses and reach a supported judgement.
Deduce
Draw a conclusion from the information given.
Justify
Support a statement/choice with evidence.

Section 19 of 19Exam technique

1
Saying yeast respires only with oxygen.
2
Saying baking kills the alcohol with heat of enzymes.
3
Saying yoghurt bacteria make carbon dioxide.
4
Saying the oil layer supplies oxygen.
1
Saying fertilisers feed the plant — plants make their own food; fertilisers supply mineral ions such as nitrate and magnesium.
2
Mixing up restriction enzyme (cuts) and ligase (joins).
3
Saying a GMO and a transgenic organism are always identical — transgenic organisms carry DNA from a different species and are a sub-group of GMOs.
4
Writing that biological control uses chemicals — it uses living predators or parasites.

Last updated: