5.1Use of Biological Resources
Food production — Edexcel Modular - OL Revision Notes & Topical Questions
15 min read 19 parts Lesson 3 of 5 2 quizzes
Food production Edexcel O Level Modular Biology notes: describe how glasshouses and polythene tunnels can be used to increase the yield of certain cropsNot all microorganisms are harmful – many are useful.Yeast needs warmth, food and moisture to work.Too much heat kills yeast.des…
- Food production Edexcel O Level Modular Biology: what to do next
- Fermentation
- Carbon dioxide
- Lactic acid
- Pasteurisation
- Give / State / Name
Section 1 of 19Process & sequence
- Sugar
- Yeast
- Carbon dioxide
- Dough rises
Section 2 of 19Keep going
Food production Edexcel O Level Modular Biology: what to do next
These Food production Edexcel O Level Modular Biology notes covered the key ideas, worked examples and exam technique for this topic. Check your Food production Edexcel O Level Modular Biology understanding with the practice questions below, then reread any Food production Edexcel O Level Modular Biology point you were unsure about before moving on.
Section 3 of 19Yeast and bread
Microorganisms can be used by humans to produce foods and other useful substances.
Section 4 of 19Practical: anaerobic respiration in yeast
Yeast can respire anaerobically (without oxygen), breaking down glucose to produce ethanol and carbon dioxide. Anaerobic respiration in yeast cells is called fermentation. Equation: glucose → ethanol + carbon dioxide.
As respiration is an enzyme-controlled reaction, as enzyme activity increases with temperature the rate of carbon dioxide production increases.
- and suggest improvements.
- (for example a gas syringe for more accurate volumes and a thermostatically controlled water bath)
Section 5 of 19Role of bacteria in yoghurt production
As well as fungi, bacteria are also used in the production of certain foods.
- which lowers the pH, causes the milk proteins to thicken, and gives yoghurt its sour taste.
Section 6 of 19Fermenters
Fermenters are containers used to grow microorganisms such as bacteria and fungi.
- such as producing genetically modified bacteria and moulds that produce insulin and antibiotics.
| Condition | Control |
|---|---|
| Optimum temperature | Monitored with probes and maintained using the water jacket; enzymes denature if the temperature is too high and work slowly if too low |
| Optimum pH | Monitored with a probe, adjusted using acids or alkalis |
| Oxygenation | Oxygen is needed for aerobic respiration, supplied by sterile air |
| Agitation | Stirring paddles ensure microorganisms, nutrients, oxygen, temperature and pH are evenly distributed |
Section 7 of 19Comparing aerobic and anaerobic respiration in yeast
| Aerobic | Anaerobic (fermentation) | |
|---|---|---|
| Oxygen | Needed | Not needed |
| Products | Carbon dioxide and water | Ethanol and carbon dioxide |
| Energy released | Much more per glucose | Much less |
| Use | Rapid growth of yeast | Bread, beer, wine |
In brewing the carbon dioxide escapes while ethanol builds up until the concentration is high enough to kill the yeast. Wine and beer production uses the same reaction, with yeast fermenting sugars from grapes or malted barley. Industrial fermenters are sterilised before use, because contaminating microbes would compete with the culture and could spoil the product.
Section 8 of 19Compare
| Food | Microorganism | Starts with | Microbe produces | Effect on food | Conditions |
|---|---|---|---|---|---|
| Bread | Yeast | Flour, sugar, water | Carbon dioxide + alcohol | Dough rises; alcohol evaporates on baking | Warm |
| Yoghurt | Bacteria (Lactobacillus) | Milk | Lactic acid | Milk thickens, tastes sour | ~40 °C |
| Cheese | Bacteria (+ moulds) | Milk | Lactic acid | Milk solidifies into curds | Controlled ripening |
| Vinegar | Bacteria | Wine / cider | Ethanoic acid | Sour taste | With oxygen |
| Soy sauce | Fungi + bacteria | Soybeans, wheat | Flavour compounds | Salty savoury taste | Months of fermentation |
Section 9 of 19Worked examples
Explain why bread dough rises. [3]3 marks
Yeast respires making carbon dioxide gas, which forms bubbles in the dough and expands it.
Describe the effect on yeast respiration of raising the temperature from 20 C to 60 C. [3]3 marks
Rate increases to the optimum (about 35-40 C) then falls as enzymes denature, stopping at high temperature.
Why is oil added to the sugar solution? [2]2 marks
To stop oxygen entering so yeast can only respire anaerobically.
List two conditions controlled in a fermenter and say why. [4]4 marks
Temperature (enzyme activity) and pH (enzyme activity); also oxygen for aerobic respiration.
Section 10 of 19Crop plants in glasshouses and polythene tunnels
If a plant is given unlimited light, carbon dioxide and water at a warm temperature, the only thing limiting the rate of photosynthesis is its own ability to absorb these materials and make them react. Glasshouses and polythene tunnels let growers control these limiting factors to raise yield.
- Temperature heaters or the trapped heat of the sun keep the enzymes of photosynthesis working at their optimum.
- Light extra lighting extends the day length.
- Carbon dioxide burners or gas supplies raise its concentration.
- Water irrigation prevents shortage.
- Pests and diseases the enclosed space keeps most pests out.
The specification requires only that magnesium ions are needed for chlorophyll and nitrate ions for amino acids, so learn those two functions. Fertilisers supply them.
Section 11 of 19Pest control: pesticides and biological control
Another key way modern technology has increased food supply is the use of pesticides, which kill insects, weeds and fungi that reduce yield. Pesticides can harm other organisms and build up in food chains.
Biological control uses a natural predator or parasite to control a pest. This can happen naturally, for example ladybirds eat aphids. It leaves no chemical residues and is long lasting, but control is slower and the introduced species may become a pest itself.
Section 12 of 19Fish farming
Fish stocks in the wild are falling because of overfishing, so many fish are now farmed in ponds, cages or tanks.
- including that stocks of wild fish are protected, the fish can be fed to grow quickly, the conditions are controlled,
- (oxygen, temperature, disease control)
- and predators and competitors are removed.
Section 13 of 19Genetic modification and biotechnology
Genetic modification (genetic engineering) is transferring a gene, or a piece of DNA, into another organism so that it gains a new characteristic. A student can remember the enzymes with a simple memory aid: a restriction enzyme is scissors (it cuts DNA at a specific sequence) and ligase is glue (it joins the cut pieces into one molecule).
| Technique | Purpose | Key steps / tool | Example use |
|---|---|---|---|
| PCR | Copy DNA | 95 °C denature, 55 °C anneal, 72 °C extend; Taq polymerase | Forensics |
| Gel electrophoresis | Separate fragments | Load, run current, stain; agarose gel | DNA fingerprinting |
| Restriction digest | Cut DNA | Mix DNA with restriction enzyme | Cloning (sticky ends) |
| Bacterial transformation | Insert a gene | Heat shock, select on antibiotic; plasmid and ligase | Insulin production |
| Sequencing | Read DNA | Dideoxy / next-generation; DNA polymerase | Genome projects |
| CRISPR | Edit genes | Guide RNA targets DNA; Cas9 | Gene therapy research |
A transgenic organism contains DNA from a different species, so every transgenic organism is a GMO, but a GMO may have DNA from the same species.
Section 14 of 19Evaluating GM crops and microorganisms in food
| Aspect | Advantages | Disadvantages |
|---|---|---|
| Chemicals | Fewer pesticides and herbicides needed | Dependence on particular herbicides |
| Cost | Cheaper for farmers to grow | The seeds themselves are expensive |
| Yield | Increased yield | Some GM crops grow less well |
| Environment | Overall benefit from reduced chemical use | Gene transfer to wild relatives can reduce biodiversity |
Microorganisms such as yeast and bacteria can be used by humans to produce foods and other useful substances. In a yeast fermentation practical, carefully adding a layer of oil on top of the solution excludes oxygen, so the yeast respires anaerobically and no further aerobic respiration takes place.
When an exam says "compare", each point must refer to both items; when it says "explain", each marking point is usually a cause followed by a consequence, and unlinked facts often score nothing.
Section 15 of 19Worked examples
Give two concerns people raise about growing GM crops. [2]2 marks
Genes may transfer to wild relatives, reducing biodiversity and affecting wildflowers and insects; reliance on specific herbicides may increase, with possible effects on human health. The seeds are also expensive for farmers.
Explain why a glasshouse increases crop yield. [3]3 marks
Heating, lighting, carbon dioxide and watering are controlled to remove limiting factors, so photosynthesis, and therefore growth, occurs at a faster rate.
State the roles of restriction enzymes and ligase in genetic modification. [2]2 marks
Restriction enzymes cut DNA at a specific sequence; ligase joins the pieces into one molecule.
Give two advantages of fish farming compared with catching wild fish. [2]2 marks
Wild stocks are protected; conditions such as feeding, oxygen and disease control can be managed for faster growth.
Why is a layer of oil added on top of a yeast fermentation experiment? [1]1 mark
To exclude oxygen so that only anaerobic respiration occurs.
Section 16 of 19Key terms
| Term | Meaning |
|---|---|
| Fermentation | Anaerobic respiration in yeast making ethanol and CO2. |
| Mycoprotein | Protein food made from fungus. |
| Agitation | Stirring in a fermenter. |
| Optimum temperature | Temperature at which enzymes work fastest. |
| Lactic acid | Acid made by yoghurt bacteria. |
| Term | Meaning |
|---|---|
| Limiting factor | A factor in short supply that restricts the rate of a process such as photosynthesis. |
| Biological control | Using a living organism, such as a predator, to control a pest. |
| Genetic modification | Transferring a gene into another organism so it gains a new characteristic. |
| Restriction enzyme | An enzyme that cuts DNA at a specific base sequence. |
| Ligase | An enzyme that joins pieces of DNA. |
| Transgenic organism | An organism containing DNA from a different species. |
| Pesticide | A chemical that kills pests. |
Section 17 of 19Syllabus checklist
- including maintaining water quality, controlling intraspecific and interspecific predation, controlling disease, removing waste products, controlling the quality and frequency of feeding,
- and selective breeding
Section 18 of 19Command terms
Section 19 of 19Exam technique
Marked practiceQuizzes for this lesson
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