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8.4Transport in Plants

Translocation Core — Cambridge IGCSE Biology 0610 Revision Notes & Topical Questions

5 min read 15 parts Lesson 7 of 9 Unit quiz

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8.4 Translocation — IGCSE BIOLOGY notes: Describe translocation of sucrose and amino acids in phloem from sources to sinks (Supplement).Sugars — Mainly sucrose, made from glucose produced in photosynthesis.Amino acids — Made using glucose and nitrates absorbed from the soil.Trans…

  • Sieve plates
  • Changing direction
  • Cells — The tubes are made of living cells, unlike xylem vessels which are dead
  • Source — A part of the plant where substances are made or released
  • Sink — A part of the plant where substances are stored or used
  • Direction — Substances always move from a source to a sink

Section 1 of 15What is translocated

Sugars
  • Mainly sucrose, made from glucose produced in photosynthesis.
Amino acids
  • Made using glucose and nitrates absorbed from the soil.
Translocation
  • The transport of sucrose and amino acids in the phloem from regions of production to regions of storage or use.

Section 2 of 15Phloem tubes

Cells
  • The tubes are made of living cells, unlike xylem vessels which are dead.
Sieve plates
  • Cells are joined end to end with holes in the end walls that let substances flow from cell to cell.

Section 3 of 15Sources and sinks

Source
  • A part of the plant where substances are made or released.
Sink
  • A part of the plant where substances are stored or used.
Direction
  • Substances always move from a source to a sink.
Changing direction
  • The direction depends on the stage of development or the time of year.

Section 4 of 15Changes through the year

TimeSourceSink
WinterStorage organsOther parts of the plant, for respiration and repair
Spring growthStorage organs such as rootsGrowing areas
SummerPhotosynthesising leavesRoots, where sucrose becomes glucose and is stored as starch

Section 5 of 15Xylem and phloem compared

TissueWhat is movedProcessDirectionCells
XylemWater and mineral ionsTranspiration streamOne way, roots to leavesDead
PhloemSucrose and amino acidsTranslocationAll directionsLiving

Section 6 of 15More from the specification

Mesophyll
  • The tissue in the middle of a leaf where photosynthesis takes place.
Movement
  • An action by an organism or part of it causing a change of position or place.
Stomata
  • Pores in the leaf through which water vapour leaves.
Humidity
  • The amount of water vapour in the air.

Section 7 of 15What translocation is

Translocation
Translocation the movement of organic solutes, mainly sucrose and amino acids, through the phloem from regions of production to regions of use or storage.
Energy
  • Energy unlike the transpiration stream it uses energy.
Direction
  • Direction it moves in both directions, always from a source to a sink.
Sucrose
  • Sucrose glucose from photosynthesis is converted to sucrose, which is soluble, transportable and does not react on the way.
Amino acids
  • Amino acids made using glucose and nitrate absorbed from the soil.

Section 8 of 15Sources and sinks

SourceSink
MeaningReleases sugars into the phloemRemoves sugars from the phloem
ExamplesGreen leaves; storage organs releasing stores in springRoots, growing shoots, flowers, fruits, tubers and bulbs

Section 9 of 15Seasonal pattern of translocation

Winter
  • Winter storage organs supply sucrose for respiration and amino acids for cell repair.
Spring
  • Spring roots are the source and growing areas are the sinks.
Summer
  • Summer leaves photosynthesise fast and are the source;
    • roots are the sink, where sucrose is converted to glucose and stored as starch.
Storage
  • Storage insoluble starch does not affect water potential, so cells do not swell or burst.

Section 10 of 15Movement of sap (pressure-flow)

1
Sucrose is actively loaded into the sieve tubes at the source, using energy from companion cells.
2
Water enters the sieve tubes by osmosis, raising the pressure at the source.
3
Sap flows along the tube towards the sink, where pressure is lower.
4
Sucrose is unloaded at the sink and used for respiration, growth or stored as starch.

Section 11 of 15Loading and unloading in more detail

1Loading
  • Loading sucrose moves from mesophyll cells into companion cells and then into the sieve tube.
  • The companion cell uses ATP to pump hydrogen ions out;
    • they return through a co-transport protein carrying sucrose in.
2Source end
  • Source end the concentrated sap has a low water potential, so water enters from nearby xylem by osmosis and raises the pressure.
3Sink end
  • Sink end sucrose is removed and converted to starch or used in respiration,
  • so the sap has a higher water potential and water leaves by osmosis,
  • giving low pressure.
4Mass flow
  • Mass flow the pressure difference drives sap along the tube, and removal of sucrose at the sink maintains the gradient.

Section 12 of 15Experimental evidence

EvidenceWhat it shows
Ringing experiment (ring of bark including phloem removed)Sugars accumulate above the ring and the bark swells, so phloem carries sugars
Radioactive tracer (leaf given 14CO2)Labelled sucrose is made and later found in other parts, mainly in the phloem
Aphid styletsSap collected from the phloem is rich in sugar, with higher concentration nearer the leaf
Metabolic poisonsFlow slows when respiration is blocked, so energy is needed

Section 13 of 15Source and sink are functions

Source and sink are functions
Summer storage
  • Summer storage plants make more sugar than they need and store the excess as starch in roots, tubers, bulbs and seeds.
Spring
  • Spring enzymes break the starch back to soluble sugars, which are translocated to buds, so the storage organ becomes a source.
Meaning
  • Meaning source and sink describe the function at a given time, not a fixed place.

Section 14 of 15Key terms

TermMeaning
TranslocationMovement of sucrose and amino acids in phloem from sources to sinks.
SourceA part of a plant that releases sucrose or amino acids.
SinkA part of a plant that uses or stores sucrose or amino acids.
SucroseThe sugar mainly transported in phloem.
Sieve platePerforated end wall of a phloem cell.
PhloemLiving tissue that transports sucrose and amino acids.
TermMeaning
TranslocationMovement of sucrose and amino acids in the phloem.
SourceRegion where sugars enter the phloem.
SinkRegion where sugars leave the phloem.
SucroseThe soluble sugar used for transport in plants.
Sieve tubeA living tube in phloem that carries sap.

Section 15 of 15Exam technique

1
Saying a plant part is always a source or always a sink — a storage organ can be both at different times.
2
Saying translocation moves only downwards — it moves from sources to sinks in any direction.
3
Saying phloem carries water and minerals — it carries sucrose and amino acids.
4
Calling phloem tubes dead — they are living cells.
1
Saying translocation is in the xylem — it is in the phloem.
2
Thinking sugars move only down to roots — they move to wherever they are needed.
3
Saying translocation is passive — it needs energy.
4
Confusing glucose with sucrose as the transport sugar — plants transport sucrose.

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