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Chapter 2 - Hormonal Coordination in Living Organisms

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Chapter 3 - Reproduction in Living Organisms

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Chapter 4 - Immunity in Living Organisms

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Chapter 5 - Molecular Biology - DNA Structure and Function

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Quiz

Lesson 2: Recombinant DNA, Gene Cloning, PCR and Genetic Engineering — Picture questions (exam style)

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  • Questions count: 300
  • Passing grade: 60%
Start Quiz
1.
A linear DNA of 8000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A linear DNA of 8000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 2, fragments with both = 2
double digest in lane 3, fragments with both = 3
double digest in lane 2, fragments with both = 3
double digest in lane 1, fragments with both = 4
2.
The graph shows the temperature profile of two PCR cycles, starting with 3 DNA molecule(s). Which option is correct?
The graph shows the temperature profile of two PCR cycles, starting with 3 DNA molecule(s). Which option is correct?
step X: extension by Taq polymerase, copies after 7 cycles = 192, time for 7 cycles = 12.8 min
step X: extension by Taq polymerase, copies after 7 cycles = 384, time for 7 cycles = 12.8 min
step X: denaturation, copies after 7 cycles = 42, time for 7 cycles = 25.7 min
step X: primer annealing, copies after 7 cycles = 384, time for 7 cycles = 25.7 min
3.
A linear DNA of 3000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A linear DNA of 3000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 1, fragments with both = 3
double digest in lane 2, fragments with both = 5
double digest in lane 3, fragments with both = 4
double digest in lane 3, fragments with both = 5
4.
The graph shows the temperature profile of two PCR cycles, starting with 2 DNA molecule(s). Which option is correct?
The graph shows the temperature profile of two PCR cycles, starting with 2 DNA molecule(s). Which option is correct?
step X: extension by Taq polymerase, copies after 7 cycles = 128, time for 7 cycles = 19.2 min
step X: extension by Taq polymerase, copies after 7 cycles = 256, time for 7 cycles = 19.2 min
step X: primer annealing, copies after 7 cycles = 28, time for 7 cycles = 38.5 min
step X: extension by Taq polymerase, copies after 7 cycles = 256, time for 7 cycles = 26.2 min
5.
A linear DNA of 5000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A linear DNA of 5000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 1, fragments with both = 6
double digest in lane 2, fragments with both = 5
double digest in lane 3, fragments with both = 5
double digest in lane 2, fragments with both = 4
6.
The graph shows the temperature profile of two PCR cycles, starting with 2 DNA molecule(s). Which option is correct?
The graph shows the temperature profile of two PCR cycles, starting with 2 DNA molecule(s). Which option is correct?
step X: denaturation, copies after 4 cycles = 32, time for 4 cycles = 15.3 min
step X: denaturation, copies after 4 cycles = 32, time for 4 cycles = 11.3 min
step X: denaturation, copies after 4 cycles = 18, time for 4 cycles = 2.83 min
step X: primer annealing, copies after 4 cycles = 32, time for 4 cycles = 11.3 min
7.
A linear DNA of 5000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A linear DNA of 5000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 2, fragments with both = 5
double digest in lane 3, fragments with both = 3
double digest in lane 2, fragments with both = 4
double digest in lane 3, fragments with both = 4
8.
The graph shows the temperature profile of two PCR cycles, starting with 2 DNA molecule(s). Which option is correct?
The graph shows the temperature profile of two PCR cycles, starting with 2 DNA molecule(s). Which option is correct?
step X: primer annealing, copies after 10 cycles = 1026, time for 10 cycles = 30.8 min
step X: denaturation, copies after 10 cycles = 1024, time for 10 cycles = 2.08 min
step X: extension by Taq polymerase, copies after 10 cycles = 2048, time for 10 cycles = 20.8 min
step X: extension by Taq polymerase, copies after 10 cycles = 2048, time for 10 cycles = 30.8 min
9.
The graph shows the temperature profile of two PCR cycles, starting with 5 DNA molecule(s). Which option is correct?
The graph shows the temperature profile of two PCR cycles, starting with 5 DNA molecule(s). Which option is correct?
step X: denaturation, copies after 5 cycles = 80, time for 5 cycles = 10 min
step X: extension by Taq polymerase, copies after 5 cycles = 50, time for 5 cycles = 20 min
step X: extension by Taq polymerase, copies after 5 cycles = 50, time for 5 cycles = 2 min
step X: extension by Taq polymerase, copies after 5 cycles = 160, time for 5 cycles = 10 min
10.
A linear DNA of 4000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A linear DNA of 4000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 2, fragments with both = 3
double digest in lane 3, fragments with both = 3
double digest in lane 2, fragments with both = 4
double digest in lane 2, fragments with both = 5
11.
The graph shows the temperature profile of two PCR cycles, starting with 1 DNA molecule(s). Which option is correct?
The graph shows the temperature profile of two PCR cycles, starting with 1 DNA molecule(s). Which option is correct?
step X: primer annealing, copies after 11 cycles = 2048, time for 11 cycles = 18.3 min
step X: extension by Taq polymerase, copies after 11 cycles = 2048, time for 11 cycles = 1.67 min
step X: primer annealing, copies after 11 cycles = 2048, time for 11 cycles = 36.7 min
step X: denaturation, copies after 11 cycles = 22, time for 11 cycles = 18.3 min
12.
A linear DNA of 8000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A linear DNA of 8000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 3, fragments with both = 5
double digest in lane 1, fragments with both = 6
double digest in lane 1, fragments with both = 4
double digest in lane 1, fragments with both = 5
13.
The graph shows the temperature profile of two PCR cycles, starting with 10 DNA molecule(s). Which option is correct?
The graph shows the temperature profile of two PCR cycles, starting with 10 DNA molecule(s). Which option is correct?
step X: extension by Taq polymerase, copies after 10 cycles = 10240, time for 10 cycles = 19.2 min
step X: primer annealing, copies after 10 cycles = 200, time for 10 cycles = 29.2 min
step X: denaturation, copies after 10 cycles = 10240, time for 10 cycles = 1.92 min
step X: extension by Taq polymerase, copies after 10 cycles = 10240, time for 10 cycles = 29.2 min
14.
A linear DNA of 8000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A linear DNA of 8000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 1, fragments with both = 4
double digest in lane 1, fragments with both = 5
double digest in lane 3, fragments with both = 6
double digest in lane 3, fragments with both = 5
15.
The graph shows the temperature profile of two PCR cycles, starting with 5 DNA molecule(s). Which option is correct?
The graph shows the temperature profile of two PCR cycles, starting with 5 DNA molecule(s). Which option is correct?
step X: denaturation, copies after 6 cycles = 320, time for 6 cycles = 12.5 min
step X: denaturation, copies after 6 cycles = 160, time for 6 cycles = 2.08 min
step X: extension by Taq polymerase, copies after 6 cycles = 320, time for 6 cycles = 12.5 min
step X: denaturation, copies after 6 cycles = 160, time for 6 cycles = 12.5 min
16.
A linear DNA of 8000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A linear DNA of 8000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 3, fragments with both = 5
double digest in lane 1, fragments with both = 4
double digest in lane 3, fragments with both = 4
double digest in lane 2, fragments with both = 6
17.
A circular plasmid of 4000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A circular plasmid of 4000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 2, fragments with both = 2
double digest in lane 3, fragments with both = 3
double digest in lane 3, fragments with both = 2
double digest in lane 1, fragments with both = 4
18.
The graph shows the temperature profile of two PCR cycles, starting with 2 DNA molecule(s). Which option is correct?
The graph shows the temperature profile of two PCR cycles, starting with 2 DNA molecule(s). Which option is correct?
step X: denaturation, copies after 7 cycles = 256, time for 7 cycles = 17.5 min
step X: primer annealing, copies after 7 cycles = 128, time for 7 cycles = 24.5 min
step X: extension by Taq polymerase, copies after 7 cycles = 28, time for 7 cycles = 17.5 min
step X: primer annealing, copies after 7 cycles = 256, time for 7 cycles = 17.5 min
19.
A linear DNA of 3000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A linear DNA of 3000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 3, fragments with both = 5
double digest in lane 2, fragments with both = 4
double digest in lane 3, fragments with both = 6
double digest in lane 2, fragments with both = 5
20.
The graph shows the temperature profile of two PCR cycles, starting with 10 DNA molecule(s). Which option is correct?
The graph shows the temperature profile of two PCR cycles, starting with 10 DNA molecule(s). Which option is correct?
step X: primer annealing, copies after 4 cycles = 26, time for 4 cycles = 9.33 min
step X: denaturation, copies after 4 cycles = 160, time for 4 cycles = 13.3 min
step X: primer annealing, copies after 4 cycles = 160, time for 4 cycles = 9.33 min
step X: primer annealing, copies after 4 cycles = 26, time for 4 cycles = 13.3 min
21.
A circular plasmid of 6000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A circular plasmid of 6000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 2, fragments with both = 1
double digest in lane 3, fragments with both = 2
double digest in lane 2, fragments with both = 3
double digest in lane 1, fragments with both = 2
22.
The graph shows the temperature profile of two PCR cycles, starting with 10 DNA molecule(s). Which option is correct?
The graph shows the temperature profile of two PCR cycles, starting with 10 DNA molecule(s). Which option is correct?
step X: extension by Taq polymerase, copies after 8 cycles = 2560, time for 8 cycles = 20.7 min
step X: extension by Taq polymerase, copies after 8 cycles = 266, time for 8 cycles = 2.58 min
step X: denaturation, copies after 8 cycles = 2560, time for 8 cycles = 20.7 min
step X: primer annealing, copies after 8 cycles = 2560, time for 8 cycles = 20.7 min
23.
A linear DNA of 8000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A linear DNA of 8000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 3, fragments with both = 5
double digest in lane 1, fragments with both = 4
double digest in lane 2, fragments with both = 5
double digest in lane 2, fragments with both = 4
24.
The graph shows the temperature profile of two PCR cycles, starting with 10 DNA molecule(s). Which option is correct?
The graph shows the temperature profile of two PCR cycles, starting with 10 DNA molecule(s). Which option is correct?
step X: primer annealing, copies after 7 cycles = 640, time for 7 cycles = 17.5 min
step X: extension by Taq polymerase, copies after 7 cycles = 1280, time for 7 cycles = 17.5 min
step X: extension by Taq polymerase, copies after 7 cycles = 1280, time for 7 cycles = 2.5 min
step X: extension by Taq polymerase, copies after 7 cycles = 640, time for 7 cycles = 35 min
25.
A linear DNA of 3000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A linear DNA of 3000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 2, fragments with both = 4
double digest in lane 3, fragments with both = 3
double digest in lane 1, fragments with both = 3
double digest in lane 1, fragments with both = 5
26.
The graph shows the temperature profile of two PCR cycles, starting with 2 DNA molecule(s). Which option is correct?
The graph shows the temperature profile of two PCR cycles, starting with 2 DNA molecule(s). Which option is correct?
step X: denaturation, copies after 5 cycles = 20, time for 5 cycles = 15 min
step X: primer annealing, copies after 5 cycles = 64, time for 5 cycles = 10 min
step X: denaturation, copies after 5 cycles = 20, time for 5 cycles = 20 min
step X: extension by Taq polymerase, copies after 5 cycles = 34, time for 5 cycles = 15 min
27.
A circular plasmid of 3000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A circular plasmid of 3000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 2, fragments with both = 2
double digest in lane 2, fragments with both = 3
double digest in lane 3, fragments with both = 3
double digest in lane 1, fragments with both = 2
28.
A linear DNA of 6000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A linear DNA of 6000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 1, fragments with both = 4
double digest in lane 3, fragments with both = 6
double digest in lane 1, fragments with both = 5
double digest in lane 3, fragments with both = 4
29.
The graph shows the temperature profile of two PCR cycles, starting with 2 DNA molecule(s). Which option is correct?
The graph shows the temperature profile of two PCR cycles, starting with 2 DNA molecule(s). Which option is correct?
step X: extension by Taq polymerase, copies after 9 cycles = 36, time for 9 cycles = 2.33 min
step X: extension by Taq polymerase, copies after 9 cycles = 1024, time for 9 cycles = 21 min
step X: denaturation, copies after 9 cycles = 512, time for 9 cycles = 2.33 min
step X: denaturation, copies after 9 cycles = 36, time for 9 cycles = 2.33 min
30.
A linear DNA of 6000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A linear DNA of 6000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 1, fragments with both = 4
double digest in lane 2, fragments with both = 3
double digest in lane 3, fragments with both = 5
double digest in lane 2, fragments with both = 4
31.
The graph shows the temperature profile of two PCR cycles, starting with 3 DNA molecule(s). Which option is correct?
The graph shows the temperature profile of two PCR cycles, starting with 3 DNA molecule(s). Which option is correct?
step X: extension by Taq polymerase, copies after 6 cycles = 192, time for 6 cycles = 16 min
step X: extension by Taq polymerase, copies after 6 cycles = 192, time for 6 cycles = 8 min
step X: primer annealing, copies after 6 cycles = 67, time for 6 cycles = 8 min
step X: denaturation, copies after 6 cycles = 67, time for 6 cycles = 16 min
32.
A linear DNA of 8000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A linear DNA of 8000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 1, fragments with both = 4
double digest in lane 1, fragments with both = 3
double digest in lane 3, fragments with both = 5
double digest in lane 3, fragments with both = 3
33.
A linear DNA of 6000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A linear DNA of 6000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 1, fragments with both = 2
double digest in lane 2, fragments with both = 3
double digest in lane 2, fragments with both = 2
double digest in lane 2, fragments with both = 4
34.
The graph shows the temperature profile of two PCR cycles, starting with 3 DNA molecule(s). Which option is correct?
The graph shows the temperature profile of two PCR cycles, starting with 3 DNA molecule(s). Which option is correct?
step X: primer annealing, copies after 7 cycles = 192, time for 7 cycles = 37.3 min
step X: primer annealing, copies after 7 cycles = 384, time for 7 cycles = 18.7 min
step X: extension by Taq polymerase, copies after 7 cycles = 192, time for 7 cycles = 2.67 min
step X: primer annealing, copies after 7 cycles = 192, time for 7 cycles = 25.7 min
35.
A circular plasmid of 6000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A circular plasmid of 6000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 2, fragments with both = 3
double digest in lane 3, fragments with both = 3
double digest in lane 1, fragments with both = 2
double digest in lane 1, fragments with both = 3
36.
The graph shows the temperature profile of two PCR cycles, starting with 5 DNA molecule(s). Which option is correct?
The graph shows the temperature profile of two PCR cycles, starting with 5 DNA molecule(s). Which option is correct?
step X: extension by Taq polymerase, copies after 9 cycles = 2560, time for 9 cycles = 22.5 min
step X: primer annealing, copies after 9 cycles = 1280, time for 9 cycles = 22.5 min
step X: primer annealing, copies after 9 cycles = 2560, time for 9 cycles = 13.5 min
step X: extension by Taq polymerase, copies after 9 cycles = 1280, time for 9 cycles = 1.5 min
37.
The graph shows the temperature profile of two PCR cycles, starting with 3 DNA molecule(s). Which option is correct?
The graph shows the temperature profile of two PCR cycles, starting with 3 DNA molecule(s). Which option is correct?
step X: extension by Taq polymerase, copies after 12 cycles = 12288, time for 12 cycles = 38 min
step X: primer annealing, copies after 12 cycles = 4099, time for 12 cycles = 38 min
step X: denaturation, copies after 12 cycles = 12288, time for 12 cycles = 50 min
step X: denaturation, copies after 12 cycles = 6144, time for 12 cycles = 76 min
38.
The graph shows the temperature profile of two PCR cycles, starting with 5 DNA molecule(s). Which option is correct?
The graph shows the temperature profile of two PCR cycles, starting with 5 DNA molecule(s). Which option is correct?
step X: denaturation, copies after 12 cycles = 20480, time for 12 cycles = 20 min
step X: denaturation, copies after 12 cycles = 4101, time for 12 cycles = 32 min
step X: extension by Taq polymerase, copies after 12 cycles = 20480, time for 12 cycles = 32 min
step X: denaturation, copies after 12 cycles = 120, time for 12 cycles = 20 min
39.
The graph shows the temperature profile of two PCR cycles, starting with 5 DNA molecule(s). Which option is correct?
The graph shows the temperature profile of two PCR cycles, starting with 5 DNA molecule(s). Which option is correct?
step X: extension by Taq polymerase, copies after 11 cycles = 110, time for 11 cycles = 47.7 min
step X: extension by Taq polymerase, copies after 11 cycles = 10240, time for 11 cycles = 23.8 min
step X: primer annealing, copies after 11 cycles = 110, time for 11 cycles = 23.8 min
step X: extension by Taq polymerase, copies after 11 cycles = 2053, time for 11 cycles = 23.8 min
40.
A circular plasmid of 5000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A circular plasmid of 5000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 1, fragments with both = 4
double digest in lane 2, fragments with both = 5
double digest in lane 2, fragments with both = 3
double digest in lane 2, fragments with both = 4
41.
The graph shows the temperature profile of two PCR cycles, starting with 5 DNA molecule(s). Which option is correct?
The graph shows the temperature profile of two PCR cycles, starting with 5 DNA molecule(s). Which option is correct?
step X: denaturation, copies after 7 cycles = 640, time for 7 cycles = 17.5 min
step X: extension by Taq polymerase, copies after 7 cycles = 640, time for 7 cycles = 17.5 min
step X: denaturation, copies after 7 cycles = 133, time for 7 cycles = 24.5 min
step X: denaturation, copies after 7 cycles = 320, time for 7 cycles = 24.5 min
42.
A linear DNA of 4000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A linear DNA of 4000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 3, fragments with both = 5
double digest in lane 2, fragments with both = 6
double digest in lane 1, fragments with both = 6
double digest in lane 3, fragments with both = 4
43.
The graph shows the temperature profile of two PCR cycles, starting with 2 DNA molecule(s). Which option is correct?
The graph shows the temperature profile of two PCR cycles, starting with 2 DNA molecule(s). Which option is correct?
step X: primer annealing, copies after 11 cycles = 2048, time for 11 cycles = 25.7 min
step X: denaturation, copies after 11 cycles = 44, time for 11 cycles = 2.33 min
step X: primer annealing, copies after 11 cycles = 4096, time for 11 cycles = 36.7 min
step X: primer annealing, copies after 11 cycles = 4096, time for 11 cycles = 25.7 min
44.
A linear DNA of 6000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A linear DNA of 6000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 3, fragments with both = 2
double digest in lane 1, fragments with both = 3
double digest in lane 2, fragments with both = 3
double digest in lane 1, fragments with both = 2
45.
The graph shows the temperature profile of two PCR cycles, starting with 5 DNA molecule(s). Which option is correct?
The graph shows the temperature profile of two PCR cycles, starting with 5 DNA molecule(s). Which option is correct?
step X: primer annealing, copies after 12 cycles = 20480, time for 12 cycles = 21 min
step X: primer annealing, copies after 12 cycles = 120, time for 12 cycles = 33 min
step X: extension by Taq polymerase, copies after 12 cycles = 20480, time for 12 cycles = 1.75 min
step X: extension by Taq polymerase, copies after 12 cycles = 20480, time for 12 cycles = 21 min
46.
A circular plasmid of 6000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A circular plasmid of 6000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 3, fragments with both = 2
double digest in lane 2, fragments with both = 3
double digest in lane 2, fragments with both = 2
double digest in lane 1, fragments with both = 4
47.
The graph shows the temperature profile of two PCR cycles, starting with 5 DNA molecule(s). Which option is correct?
The graph shows the temperature profile of two PCR cycles, starting with 5 DNA molecule(s). Which option is correct?
step X: extension by Taq polymerase, copies after 9 cycles = 2560, time for 9 cycles = 12 min
step X: extension by Taq polymerase, copies after 9 cycles = 2560, time for 9 cycles = 21 min
step X: denaturation, copies after 9 cycles = 90, time for 9 cycles = 12 min
step X: extension by Taq polymerase, copies after 9 cycles = 1280, time for 9 cycles = 12 min
48.
A linear DNA of 5000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A linear DNA of 5000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 2, fragments with both = 2
double digest in lane 1, fragments with both = 4
double digest in lane 2, fragments with both = 3
double digest in lane 3, fragments with both = 3
49.
The graph shows the temperature profile of two PCR cycles, starting with 2 DNA molecule(s). Which option is correct?
The graph shows the temperature profile of two PCR cycles, starting with 2 DNA molecule(s). Which option is correct?
step X: primer annealing, copies after 6 cycles = 66, time for 6 cycles = 35 min
step X: extension by Taq polymerase, copies after 6 cycles = 128, time for 6 cycles = 2.92 min
step X: primer annealing, copies after 6 cycles = 128, time for 6 cycles = 17.5 min
step X: extension by Taq polymerase, copies after 6 cycles = 128, time for 6 cycles = 17.5 min
50.
A linear DNA of 3000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A linear DNA of 3000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 2, fragments with both = 2
double digest in lane 1, fragments with both = 4
double digest in lane 1, fragments with both = 2
double digest in lane 2, fragments with both = 3
51.
The graph shows the temperature profile of two PCR cycles, starting with 1 DNA molecule(s). Which option is correct?
The graph shows the temperature profile of two PCR cycles, starting with 1 DNA molecule(s). Which option is correct?
step X: extension by Taq polymerase, copies after 7 cycles = 128, time for 7 cycles = 25.7 min
step X: primer annealing, copies after 7 cycles = 128, time for 7 cycles = 12.8 min
step X: primer annealing, copies after 7 cycles = 129, time for 7 cycles = 12.8 min
step X: denaturation, copies after 7 cycles = 128, time for 7 cycles = 12.8 min
52.
A circular plasmid of 4000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A circular plasmid of 4000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 2, fragments with both = 4
double digest in lane 2, fragments with both = 3
double digest in lane 1, fragments with both = 3
double digest in lane 3, fragments with both = 2
53.
The graph shows the temperature profile of two PCR cycles, starting with 2 DNA molecule(s). Which option is correct?
The graph shows the temperature profile of two PCR cycles, starting with 2 DNA molecule(s). Which option is correct?
step X: primer annealing, copies after 12 cycles = 4096, time for 12 cycles = 34 min
step X: extension by Taq polymerase, copies after 12 cycles = 8192, time for 12 cycles = 34 min
step X: primer annealing, copies after 12 cycles = 8192, time for 12 cycles = 34 min
step X: denaturation, copies after 12 cycles = 8192, time for 12 cycles = 34 min
54.
A circular plasmid of 8000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A circular plasmid of 8000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 2, fragments with both = 3
double digest in lane 3, fragments with both = 4
double digest in lane 1, fragments with both = 2
double digest in lane 2, fragments with both = 2
55.
A linear DNA of 8000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A linear DNA of 8000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 1, fragments with both = 6
double digest in lane 2, fragments with both = 4
double digest in lane 3, fragments with both = 5
double digest in lane 2, fragments with both = 6
56.
The graph shows the temperature profile of two PCR cycles, starting with 3 DNA molecule(s). Which option is correct?
The graph shows the temperature profile of two PCR cycles, starting with 3 DNA molecule(s). Which option is correct?
step X: extension by Taq polymerase, copies after 7 cycles = 192, time for 7 cycles = 37.3 min
step X: denaturation, copies after 7 cycles = 384, time for 7 cycles = 18.7 min
step X: primer annealing, copies after 7 cycles = 384, time for 7 cycles = 18.7 min
step X: denaturation, copies after 7 cycles = 42, time for 7 cycles = 37.3 min
57.
A circular plasmid of 4000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A circular plasmid of 4000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 3, fragments with both = 3
double digest in lane 1, fragments with both = 3
double digest in lane 3, fragments with both = 4
double digest in lane 1, fragments with both = 2
58.
The graph shows the temperature profile of two PCR cycles, starting with 3 DNA molecule(s). Which option is correct?
The graph shows the temperature profile of two PCR cycles, starting with 3 DNA molecule(s). Which option is correct?
step X: primer annealing, copies after 6 cycles = 36, time for 6 cycles = 29 min
step X: denaturation, copies after 6 cycles = 192, time for 6 cycles = 14.5 min
step X: extension by Taq polymerase, copies after 6 cycles = 67, time for 6 cycles = 2.42 min
step X: denaturation, copies after 6 cycles = 67, time for 6 cycles = 14.5 min
59.
A linear DNA of 6000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A linear DNA of 6000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 2, fragments with both = 5
double digest in lane 1, fragments with both = 4
double digest in lane 3, fragments with both = 3
double digest in lane 1, fragments with both = 5
60.
A linear DNA of 4000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A linear DNA of 4000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 2, fragments with both = 3
double digest in lane 3, fragments with both = 2
double digest in lane 3, fragments with both = 3
double digest in lane 2, fragments with both = 2
61.
The graph shows the temperature profile of two PCR cycles, starting with 10 DNA molecule(s). Which option is correct?
The graph shows the temperature profile of two PCR cycles, starting with 10 DNA molecule(s). Which option is correct?
step X: denaturation, copies after 8 cycles = 266, time for 8 cycles = 22 min
step X: denaturation, copies after 8 cycles = 266, time for 8 cycles = 44 min
step X: primer annealing, copies after 8 cycles = 2560, time for 8 cycles = 22 min
step X: extension by Taq polymerase, copies after 8 cycles = 1280, time for 8 cycles = 22 min
62.
A linear DNA of 4000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A linear DNA of 4000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 2, fragments with both = 5
double digest in lane 1, fragments with both = 6
double digest in lane 3, fragments with both = 5
double digest in lane 1, fragments with both = 5
63.
The graph shows the temperature profile of two PCR cycles, starting with 1 DNA molecule(s). Which option is correct?
The graph shows the temperature profile of two PCR cycles, starting with 1 DNA molecule(s). Which option is correct?
step X: extension by Taq polymerase, copies after 10 cycles = 1024, time for 10 cycles = 21.7 min
step X: denaturation, copies after 10 cycles = 20, time for 10 cycles = 31.7 min
step X: extension by Taq polymerase, copies after 10 cycles = 20, time for 10 cycles = 31.7 min
step X: primer annealing, copies after 10 cycles = 1025, time for 10 cycles = 31.7 min
64.
A linear DNA of 8000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A linear DNA of 8000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 3, fragments with both = 3
double digest in lane 3, fragments with both = 4
double digest in lane 2, fragments with both = 3
double digest in lane 3, fragments with both = 2
65.
The graph shows the temperature profile of two PCR cycles, starting with 1 DNA molecule(s). Which option is correct?
The graph shows the temperature profile of two PCR cycles, starting with 1 DNA molecule(s). Which option is correct?
step X: extension by Taq polymerase, copies after 8 cycles = 16, time for 8 cycles = 21.3 min
step X: denaturation, copies after 8 cycles = 257, time for 8 cycles = 29.3 min
step X: denaturation, copies after 8 cycles = 256, time for 8 cycles = 21.3 min
step X: primer annealing, copies after 8 cycles = 256, time for 8 cycles = 21.3 min
66.
A circular plasmid of 4000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A circular plasmid of 4000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 1, fragments with both = 4
double digest in lane 3, fragments with both = 2
double digest in lane 2, fragments with both = 3
double digest in lane 1, fragments with both = 2
67.
The graph shows the temperature profile of two PCR cycles, starting with 2 DNA molecule(s). Which option is correct?
The graph shows the temperature profile of two PCR cycles, starting with 2 DNA molecule(s). Which option is correct?
step X: primer annealing, copies after 8 cycles = 512, time for 8 cycles = 17.3 min
step X: primer annealing, copies after 8 cycles = 256, time for 8 cycles = 2.17 min
step X: extension by Taq polymerase, copies after 8 cycles = 512, time for 8 cycles = 17.3 min
step X: extension by Taq polymerase, copies after 8 cycles = 32, time for 8 cycles = 2.17 min
68.
A linear DNA of 3000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A linear DNA of 3000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 2, fragments with both = 3
double digest in lane 1, fragments with both = 3
double digest in lane 3, fragments with both = 3
double digest in lane 3, fragments with both = 4
69.
A circular plasmid of 3000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A circular plasmid of 3000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 1, fragments with both = 2
double digest in lane 2, fragments with both = 3
double digest in lane 2, fragments with both = 1
double digest in lane 3, fragments with both = 2
70.
The graph shows the temperature profile of two PCR cycles, starting with 1 DNA molecule(s). Which option is correct?
The graph shows the temperature profile of two PCR cycles, starting with 1 DNA molecule(s). Which option is correct?
step X: denaturation, copies after 4 cycles = 17, time for 4 cycles = 7.67 min
step X: denaturation, copies after 4 cycles = 16, time for 4 cycles = 7.67 min
step X: denaturation, copies after 4 cycles = 8, time for 4 cycles = 1.92 min
step X: primer annealing, copies after 4 cycles = 16, time for 4 cycles = 1.92 min
71.
A linear DNA of 4000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A linear DNA of 4000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 3, fragments with both = 5
double digest in lane 2, fragments with both = 4
double digest in lane 1, fragments with both = 5
double digest in lane 2, fragments with both = 5
72.
The graph shows the temperature profile of two PCR cycles, starting with 5 DNA molecule(s). Which option is correct?
The graph shows the temperature profile of two PCR cycles, starting with 5 DNA molecule(s). Which option is correct?
step X: primer annealing, copies after 9 cycles = 90, time for 9 cycles = 24 min
step X: primer annealing, copies after 9 cycles = 2560, time for 9 cycles = 33 min
step X: primer annealing, copies after 9 cycles = 2560, time for 9 cycles = 24 min
step X: extension by Taq polymerase, copies after 9 cycles = 90, time for 9 cycles = 2.67 min
73.
A linear DNA of 3000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A linear DNA of 3000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 3, fragments with both = 5
double digest in lane 1, fragments with both = 3
double digest in lane 3, fragments with both = 3
double digest in lane 3, fragments with both = 4
74.
The graph shows the temperature profile of two PCR cycles, starting with 3 DNA molecule(s). Which option is correct?
The graph shows the temperature profile of two PCR cycles, starting with 3 DNA molecule(s). Which option is correct?
step X: denaturation, copies after 11 cycles = 6144, time for 11 cycles = 23.8 min
step X: extension by Taq polymerase, copies after 11 cycles = 2051, time for 11 cycles = 2.17 min
step X: primer annealing, copies after 11 cycles = 2051, time for 11 cycles = 2.17 min
step X: extension by Taq polymerase, copies after 11 cycles = 3072, time for 11 cycles = 23.8 min
75.
A circular plasmid of 8000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A circular plasmid of 8000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 2, fragments with both = 2
double digest in lane 3, fragments with both = 3
double digest in lane 3, fragments with both = 2
double digest in lane 2, fragments with both = 3
76.
A linear DNA of 4000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A linear DNA of 4000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 3, fragments with both = 4
double digest in lane 2, fragments with both = 3
double digest in lane 2, fragments with both = 4
double digest in lane 3, fragments with both = 3
77.
A circular plasmid of 5000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A circular plasmid of 5000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 1, fragments with both = 3
double digest in lane 3, fragments with both = 1
double digest in lane 2, fragments with both = 1
double digest in lane 3, fragments with both = 2
78.
The graph shows the temperature profile of two PCR cycles, starting with 1 DNA molecule(s). Which option is correct?
The graph shows the temperature profile of two PCR cycles, starting with 1 DNA molecule(s). Which option is correct?
step X: denaturation, copies after 9 cycles = 513, time for 9 cycles = 31.5 min
step X: primer annealing, copies after 9 cycles = 18, time for 9 cycles = 22.5 min
step X: denaturation, copies after 9 cycles = 512, time for 9 cycles = 22.5 min
step X: primer annealing, copies after 9 cycles = 513, time for 9 cycles = 31.5 min
79.
The graph shows the temperature profile of two PCR cycles, starting with 5 DNA molecule(s). Which option is correct?
The graph shows the temperature profile of two PCR cycles, starting with 5 DNA molecule(s). Which option is correct?
step X: extension by Taq polymerase, copies after 6 cycles = 69, time for 6 cycles = 20 min
step X: primer annealing, copies after 6 cycles = 320, time for 6 cycles = 14 min
step X: primer annealing, copies after 6 cycles = 69, time for 6 cycles = 14 min
step X: primer annealing, copies after 6 cycles = 160, time for 6 cycles = 14 min
80.
A circular plasmid of 6000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A circular plasmid of 6000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 1, fragments with both = 5
double digest in lane 1, fragments with both = 4
double digest in lane 2, fragments with both = 3
double digest in lane 3, fragments with both = 5
81.
A circular plasmid of 3000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A circular plasmid of 3000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 2, fragments with both = 3
double digest in lane 1, fragments with both = 3
double digest in lane 3, fragments with both = 3
double digest in lane 1, fragments with both = 4
82.
A linear DNA of 8000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A linear DNA of 8000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 1, fragments with both = 4
double digest in lane 1, fragments with both = 5
double digest in lane 2, fragments with both = 5
double digest in lane 2, fragments with both = 3
83.
The graph shows the temperature profile of two PCR cycles, starting with 3 DNA molecule(s). Which option is correct?
The graph shows the temperature profile of two PCR cycles, starting with 3 DNA molecule(s). Which option is correct?
step X: extension by Taq polymerase, copies after 8 cycles = 768, time for 8 cycles = 17.3 min
step X: denaturation, copies after 8 cycles = 768, time for 8 cycles = 17.3 min
step X: denaturation, copies after 8 cycles = 768, time for 8 cycles = 34.7 min
step X: denaturation, copies after 8 cycles = 768, time for 8 cycles = 25.3 min
84.
The graph shows the temperature profile of two PCR cycles, starting with 5 DNA molecule(s). Which option is correct?
The graph shows the temperature profile of two PCR cycles, starting with 5 DNA molecule(s). Which option is correct?
step X: denaturation, copies after 4 cycles = 80, time for 4 cycles = 9.33 min
step X: extension by Taq polymerase, copies after 4 cycles = 21, time for 4 cycles = 2.33 min
step X: extension by Taq polymerase, copies after 4 cycles = 21, time for 4 cycles = 9.33 min
step X: denaturation, copies after 4 cycles = 21, time for 4 cycles = 9.33 min
85.
The graph shows the temperature profile of two PCR cycles, starting with 2 DNA molecule(s). Which option is correct?
The graph shows the temperature profile of two PCR cycles, starting with 2 DNA molecule(s). Which option is correct?
step X: denaturation, copies after 9 cycles = 1024, time for 9 cycles = 30 min
step X: primer annealing, copies after 9 cycles = 514, time for 9 cycles = 30 min
step X: primer annealing, copies after 9 cycles = 1024, time for 9 cycles = 15 min
step X: denaturation, copies after 9 cycles = 1024, time for 9 cycles = 15 min
86.
A linear DNA of 4000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A linear DNA of 4000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 1, fragments with both = 3
double digest in lane 1, fragments with both = 5
double digest in lane 1, fragments with both = 4
double digest in lane 2, fragments with both = 5
87.
The graph shows the temperature profile of two PCR cycles, starting with 2 DNA molecule(s). Which option is correct?
The graph shows the temperature profile of two PCR cycles, starting with 2 DNA molecule(s). Which option is correct?
step X: denaturation, copies after 11 cycles = 4096, time for 11 cycles = 2.5 min
step X: extension by Taq polymerase, copies after 11 cycles = 4096, time for 11 cycles = 27.5 min
step X: primer annealing, copies after 11 cycles = 4096, time for 11 cycles = 38.5 min
step X: extension by Taq polymerase, copies after 11 cycles = 44, time for 11 cycles = 38.5 min
88.
A linear DNA of 6000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A linear DNA of 6000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 2, fragments with both = 4
double digest in lane 1, fragments with both = 5
double digest in lane 2, fragments with both = 6
double digest in lane 2, fragments with both = 5
89.
A linear DNA of 8000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A linear DNA of 8000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 1, fragments with both = 2
double digest in lane 2, fragments with both = 3
double digest in lane 2, fragments with both = 4
double digest in lane 3, fragments with both = 3
90.
The graph shows the temperature profile of two PCR cycles, starting with 10 DNA molecule(s). Which option is correct?
The graph shows the temperature profile of two PCR cycles, starting with 10 DNA molecule(s). Which option is correct?
step X: primer annealing, copies after 6 cycles = 640, time for 6 cycles = 24 min
step X: denaturation, copies after 6 cycles = 320, time for 6 cycles = 18 min
step X: extension by Taq polymerase, copies after 6 cycles = 120, time for 6 cycles = 18 min
step X: denaturation, copies after 6 cycles = 640, time for 6 cycles = 18 min
91.
A linear DNA of 5000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A linear DNA of 5000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 2, fragments with both = 5
double digest in lane 2, fragments with both = 4
double digest in lane 1, fragments with both = 5
double digest in lane 3, fragments with both = 4
92.
A linear DNA of 3000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A linear DNA of 3000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 1, fragments with both = 4
double digest in lane 1, fragments with both = 3
double digest in lane 2, fragments with both = 4
double digest in lane 2, fragments with both = 3
93.
The graph shows the temperature profile of two PCR cycles, starting with 10 DNA molecule(s). Which option is correct?
The graph shows the temperature profile of two PCR cycles, starting with 10 DNA molecule(s). Which option is correct?
step X: denaturation, copies after 5 cycles = 160, time for 5 cycles = 15 min
step X: denaturation, copies after 5 cycles = 320, time for 5 cycles = 7.5 min
step X: primer annealing, copies after 5 cycles = 320, time for 5 cycles = 7.5 min
step X: primer annealing, copies after 5 cycles = 42, time for 5 cycles = 1.5 min
94.
A linear DNA of 6000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A linear DNA of 6000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 3, fragments with both = 2
double digest in lane 2, fragments with both = 3
double digest in lane 3, fragments with both = 4
double digest in lane 2, fragments with both = 2
95.
A circular plasmid of 3000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A circular plasmid of 3000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 1, fragments with both = 4
double digest in lane 1, fragments with both = 3
double digest in lane 3, fragments with both = 3
double digest in lane 2, fragments with both = 4
96.
The graph shows the temperature profile of two PCR cycles, starting with 1 DNA molecule(s). Which option is correct?
The graph shows the temperature profile of two PCR cycles, starting with 1 DNA molecule(s). Which option is correct?
step X: primer annealing, copies after 10 cycles = 20, time for 10 cycles = 41.7 min
step X: denaturation, copies after 10 cycles = 1024, time for 10 cycles = 20.8 min
step X: denaturation, copies after 10 cycles = 20, time for 10 cycles = 20.8 min
step X: denaturation, copies after 10 cycles = 512, time for 10 cycles = 20.8 min
97.
A circular plasmid of 5000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A circular plasmid of 5000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 2, fragments with both = 3
double digest in lane 2, fragments with both = 2
double digest in lane 2, fragments with both = 4
double digest in lane 3, fragments with both = 4
98.
The graph shows the temperature profile of two PCR cycles, starting with 5 DNA molecule(s). Which option is correct?
The graph shows the temperature profile of two PCR cycles, starting with 5 DNA molecule(s). Which option is correct?
step X: primer annealing, copies after 9 cycles = 1280, time for 9 cycles = 19.5 min
step X: primer annealing, copies after 9 cycles = 2560, time for 9 cycles = 19.5 min
step X: primer annealing, copies after 9 cycles = 2560, time for 9 cycles = 28.5 min
step X: denaturation, copies after 9 cycles = 517, time for 9 cycles = 28.5 min
99.
A linear DNA of 5000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A linear DNA of 5000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 1, fragments with both = 4
double digest in lane 3, fragments with both = 5
double digest in lane 1, fragments with both = 5
double digest in lane 2, fragments with both = 5
100.
The graph shows the temperature profile of two PCR cycles, starting with 2 DNA molecule(s). Which option is correct?
The graph shows the temperature profile of two PCR cycles, starting with 2 DNA molecule(s). Which option is correct?
step X: denaturation, copies after 10 cycles = 40, time for 10 cycles = 18.3 min
step X: extension by Taq polymerase, copies after 10 cycles = 40, time for 10 cycles = 1.83 min
step X: primer annealing, copies after 10 cycles = 2048, time for 10 cycles = 18.3 min
step X: denaturation, copies after 10 cycles = 1024, time for 10 cycles = 18.3 min
101.
A circular plasmid of 5000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A circular plasmid of 5000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 2, fragments with both = 4
double digest in lane 1, fragments with both = 3
double digest in lane 2, fragments with both = 3
double digest in lane 1, fragments with both = 2
102.
A circular plasmid of 6000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A circular plasmid of 6000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 2, fragments with both = 3
double digest in lane 1, fragments with both = 4
double digest in lane 2, fragments with both = 2
double digest in lane 1, fragments with both = 3
103.
A linear DNA of 5000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A linear DNA of 5000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 1, fragments with both = 4
double digest in lane 1, fragments with both = 5
double digest in lane 2, fragments with both = 5
double digest in lane 2, fragments with both = 3
104.
A linear DNA of 4000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A linear DNA of 4000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 3, fragments with both = 4
double digest in lane 3, fragments with both = 6
double digest in lane 1, fragments with both = 5
double digest in lane 2, fragments with both = 6
105.
A circular plasmid of 4000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A circular plasmid of 4000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 1, fragments with both = 4
double digest in lane 2, fragments with both = 2
double digest in lane 2, fragments with both = 3
double digest in lane 1, fragments with both = 2
106.
A linear DNA of 4000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A linear DNA of 4000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 2, fragments with both = 5
double digest in lane 3, fragments with both = 4
double digest in lane 1, fragments with both = 6
double digest in lane 1, fragments with both = 5
107.
A circular plasmid of 3000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A circular plasmid of 3000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 1, fragments with both = 3
double digest in lane 2, fragments with both = 3
double digest in lane 3, fragments with both = 2
double digest in lane 2, fragments with both = 2
108.
The graph shows the temperature profile of two PCR cycles, starting with 5 DNA molecule(s). Which option is correct?
The graph shows the temperature profile of two PCR cycles, starting with 5 DNA molecule(s). Which option is correct?
step X: denaturation, copies after 5 cycles = 80, time for 5 cycles = 7.5 min
step X: extension by Taq polymerase, copies after 5 cycles = 37, time for 5 cycles = 12.5 min
step X: denaturation, copies after 5 cycles = 160, time for 5 cycles = 7.5 min
step X: primer annealing, copies after 5 cycles = 37, time for 5 cycles = 15 min
109.
A circular plasmid of 8000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A circular plasmid of 8000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 1, fragments with both = 3
double digest in lane 1, fragments with both = 4
double digest in lane 2, fragments with both = 4
double digest in lane 3, fragments with both = 4
110.
The graph shows the temperature profile of two PCR cycles, starting with 1 DNA molecule(s). Which option is correct?
The graph shows the temperature profile of two PCR cycles, starting with 1 DNA molecule(s). Which option is correct?
step X: denaturation, copies after 12 cycles = 4096, time for 12 cycles = 24 min
step X: denaturation, copies after 12 cycles = 4096, time for 12 cycles = 2 min
step X: primer annealing, copies after 12 cycles = 24, time for 12 cycles = 24 min
step X: primer annealing, copies after 12 cycles = 4096, time for 12 cycles = 36 min
111.
The graph shows the temperature profile of two PCR cycles, starting with 3 DNA molecule(s). Which option is correct?
The graph shows the temperature profile of two PCR cycles, starting with 3 DNA molecule(s). Which option is correct?
step X: extension by Taq polymerase, copies after 10 cycles = 1536, time for 10 cycles = 41.7 min
step X: primer annealing, copies after 10 cycles = 3072, time for 10 cycles = 20.8 min
step X: extension by Taq polymerase, copies after 10 cycles = 60, time for 10 cycles = 30.8 min
step X: extension by Taq polymerase, copies after 10 cycles = 3072, time for 10 cycles = 20.8 min
112.
A circular plasmid of 4000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A circular plasmid of 4000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 3, fragments with both = 4
double digest in lane 1, fragments with both = 4
double digest in lane 3, fragments with both = 3
double digest in lane 3, fragments with both = 2
113.
The graph shows the temperature profile of two PCR cycles, starting with 5 DNA molecule(s). Which option is correct?
The graph shows the temperature profile of two PCR cycles, starting with 5 DNA molecule(s). Which option is correct?
step X: primer annealing, copies after 12 cycles = 20480, time for 12 cycles = 26 min
step X: primer annealing, copies after 12 cycles = 20480, time for 12 cycles = 2.17 min
step X: denaturation, copies after 12 cycles = 120, time for 12 cycles = 52 min
step X: primer annealing, copies after 12 cycles = 120, time for 12 cycles = 52 min
114.
A linear DNA of 6000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A linear DNA of 6000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 1, fragments with both = 6
double digest in lane 1, fragments with both = 4
double digest in lane 1, fragments with both = 5
double digest in lane 3, fragments with both = 4
115.
A linear DNA of 5000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A linear DNA of 5000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 3, fragments with both = 4
double digest in lane 3, fragments with both = 5
double digest in lane 1, fragments with both = 5
double digest in lane 3, fragments with both = 3
116.
A linear DNA of 3000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A linear DNA of 3000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 2, fragments with both = 3
double digest in lane 1, fragments with both = 3
double digest in lane 1, fragments with both = 4
double digest in lane 3, fragments with both = 4
117.
The graph shows the temperature profile of two PCR cycles, starting with 5 DNA molecule(s). Which option is correct?
The graph shows the temperature profile of two PCR cycles, starting with 5 DNA molecule(s). Which option is correct?
step X: denaturation, copies after 12 cycles = 20480, time for 12 cycles = 30 min
step X: extension by Taq polymerase, copies after 12 cycles = 10240, time for 12 cycles = 60 min
step X: primer annealing, copies after 12 cycles = 4101, time for 12 cycles = 42 min
step X: extension by Taq polymerase, copies after 12 cycles = 4101, time for 12 cycles = 2.5 min
118.
A linear DNA of 4000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A linear DNA of 4000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 2, fragments with both = 2
double digest in lane 2, fragments with both = 4
double digest in lane 3, fragments with both = 4
double digest in lane 2, fragments with both = 3
119.
The graph shows the temperature profile of two PCR cycles, starting with 10 DNA molecule(s). Which option is correct?
The graph shows the temperature profile of two PCR cycles, starting with 10 DNA molecule(s). Which option is correct?
step X: denaturation, copies after 11 cycles = 20480, time for 11 cycles = 29.3 min
step X: primer annealing, copies after 11 cycles = 20480, time for 11 cycles = 2.67 min
step X: denaturation, copies after 11 cycles = 20480, time for 11 cycles = 40.3 min
step X: denaturation, copies after 11 cycles = 20480, time for 11 cycles = 2.67 min
120.
The graph shows the temperature profile of two PCR cycles, starting with 1 DNA molecule(s). Which option is correct?
The graph shows the temperature profile of two PCR cycles, starting with 1 DNA molecule(s). Which option is correct?
step X: denaturation, copies after 12 cycles = 24, time for 12 cycles = 44 min
step X: primer annealing, copies after 12 cycles = 24, time for 12 cycles = 22 min
step X: extension by Taq polymerase, copies after 12 cycles = 4096, time for 12 cycles = 22 min
step X: extension by Taq polymerase, copies after 12 cycles = 2048, time for 12 cycles = 22 min
121.
A linear DNA of 4000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A linear DNA of 4000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 2, fragments with both = 3
double digest in lane 3, fragments with both = 4
double digest in lane 2, fragments with both = 2
double digest in lane 1, fragments with both = 2
122.
A circular plasmid of 4000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A circular plasmid of 4000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 2, fragments with both = 3
double digest in lane 1, fragments with both = 2
double digest in lane 1, fragments with both = 4
double digest in lane 3, fragments with both = 4
123.
A linear DNA of 4000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A linear DNA of 4000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 1, fragments with both = 5
double digest in lane 1, fragments with both = 3
double digest in lane 3, fragments with both = 4
double digest in lane 2, fragments with both = 5
124.
A linear DNA of 8000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A linear DNA of 8000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 3, fragments with both = 6
double digest in lane 1, fragments with both = 6
double digest in lane 1, fragments with both = 4
double digest in lane 2, fragments with both = 5
125.
The graph shows the temperature profile of two PCR cycles, starting with 5 DNA molecule(s). Which option is correct?
The graph shows the temperature profile of two PCR cycles, starting with 5 DNA molecule(s). Which option is correct?
step X: denaturation, copies after 4 cycles = 21, time for 4 cycles = 21.3 min
step X: extension by Taq polymerase, copies after 4 cycles = 80, time for 4 cycles = 10.7 min
step X: extension by Taq polymerase, copies after 4 cycles = 40, time for 4 cycles = 10.7 min
step X: extension by Taq polymerase, copies after 4 cycles = 40, time for 4 cycles = 21.3 min
126.
The graph shows the temperature profile of two PCR cycles, starting with 3 DNA molecule(s). Which option is correct?
The graph shows the temperature profile of two PCR cycles, starting with 3 DNA molecule(s). Which option is correct?
step X: extension by Taq polymerase, copies after 4 cycles = 48, time for 4 cycles = 13.3 min
step X: extension by Taq polymerase, copies after 4 cycles = 48, time for 4 cycles = 9.33 min
step X: primer annealing, copies after 4 cycles = 48, time for 4 cycles = 9.33 min
step X: primer annealing, copies after 4 cycles = 24, time for 4 cycles = 9.33 min
127.
A linear DNA of 8000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A linear DNA of 8000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 1, fragments with both = 2
double digest in lane 1, fragments with both = 4
double digest in lane 3, fragments with both = 4
double digest in lane 3, fragments with both = 3
128.
A linear DNA of 6000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A linear DNA of 6000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 3, fragments with both = 3
double digest in lane 1, fragments with both = 4
double digest in lane 2, fragments with both = 3
double digest in lane 3, fragments with both = 4
129.
The graph shows the temperature profile of two PCR cycles, starting with 3 DNA molecule(s). Which option is correct?
The graph shows the temperature profile of two PCR cycles, starting with 3 DNA molecule(s). Which option is correct?
step X: extension by Taq polymerase, copies after 8 cycles = 48, time for 8 cycles = 25.3 min
step X: denaturation, copies after 8 cycles = 384, time for 8 cycles = 50.7 min
step X: extension by Taq polymerase, copies after 8 cycles = 384, time for 8 cycles = 3.17 min
step X: primer annealing, copies after 8 cycles = 768, time for 8 cycles = 25.3 min
130.
A linear DNA of 5000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A linear DNA of 5000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 1, fragments with both = 5
double digest in lane 1, fragments with both = 3
double digest in lane 3, fragments with both = 4
double digest in lane 2, fragments with both = 4
131.
A linear DNA of 3000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A linear DNA of 3000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 1, fragments with both = 4
double digest in lane 1, fragments with both = 5
double digest in lane 3, fragments with both = 3
double digest in lane 2, fragments with both = 4
132.
The graph shows the temperature profile of two PCR cycles, starting with 2 DNA molecule(s). Which option is correct?
The graph shows the temperature profile of two PCR cycles, starting with 2 DNA molecule(s). Which option is correct?
step X: primer annealing, copies after 9 cycles = 514, time for 9 cycles = 32.2 min
step X: primer annealing, copies after 9 cycles = 1024, time for 9 cycles = 2.58 min
step X: primer annealing, copies after 9 cycles = 1024, time for 9 cycles = 23.2 min
step X: denaturation, copies after 9 cycles = 512, time for 9 cycles = 46.5 min
133.
A circular plasmid of 6000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A circular plasmid of 6000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 1, fragments with both = 5
double digest in lane 1, fragments with both = 4
double digest in lane 3, fragments with both = 4
double digest in lane 2, fragments with both = 4
134.
A linear DNA of 6000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A linear DNA of 6000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 1, fragments with both = 3
double digest in lane 3, fragments with both = 2
double digest in lane 1, fragments with both = 2
double digest in lane 3, fragments with both = 3
135.
The graph shows the temperature profile of two PCR cycles, starting with 3 DNA molecule(s). Which option is correct?
The graph shows the temperature profile of two PCR cycles, starting with 3 DNA molecule(s). Which option is correct?
step X: denaturation, copies after 12 cycles = 6144, time for 12 cycles = 3.17 min
step X: denaturation, copies after 12 cycles = 12288, time for 12 cycles = 38 min
step X: primer annealing, copies after 12 cycles = 12288, time for 12 cycles = 38 min
step X: denaturation, copies after 12 cycles = 72, time for 12 cycles = 38 min
136.
A circular plasmid of 5000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A circular plasmid of 5000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 1, fragments with both = 2
double digest in lane 2, fragments with both = 3
double digest in lane 3, fragments with both = 3
double digest in lane 3, fragments with both = 2
137.
A linear DNA of 3000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A linear DNA of 3000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 1, fragments with both = 4
double digest in lane 2, fragments with both = 3
double digest in lane 2, fragments with both = 5
double digest in lane 2, fragments with both = 4
138.
A circular plasmid of 8000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A circular plasmid of 8000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 1, fragments with both = 3
double digest in lane 2, fragments with both = 3
double digest in lane 1, fragments with both = 4
double digest in lane 3, fragments with both = 3
139.
A linear DNA of 3000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A linear DNA of 3000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 1, fragments with both = 3
double digest in lane 1, fragments with both = 5
double digest in lane 3, fragments with both = 3
double digest in lane 1, fragments with both = 4
140.
A linear DNA of 8000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A linear DNA of 8000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 2, fragments with both = 3
double digest in lane 1, fragments with both = 5
double digest in lane 2, fragments with both = 4
double digest in lane 2, fragments with both = 5
141.
The graph shows the temperature profile of two PCR cycles, starting with 2 DNA molecule(s). Which option is correct?
The graph shows the temperature profile of two PCR cycles, starting with 2 DNA molecule(s). Which option is correct?
step X: primer annealing, copies after 4 cycles = 32, time for 4 cycles = 2.5 min
step X: denaturation, copies after 4 cycles = 18, time for 4 cycles = 2.5 min
step X: denaturation, copies after 4 cycles = 32, time for 4 cycles = 10 min
step X: primer annealing, copies after 4 cycles = 16, time for 4 cycles = 20 min
142.
A linear DNA of 3000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A linear DNA of 3000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 2, fragments with both = 5
double digest in lane 3, fragments with both = 5
double digest in lane 2, fragments with both = 4
double digest in lane 1, fragments with both = 5
143.
A linear DNA of 3000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A linear DNA of 3000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 2, fragments with both = 6
double digest in lane 3, fragments with both = 6
double digest in lane 3, fragments with both = 5
double digest in lane 2, fragments with both = 5
144.
A circular plasmid of 6000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A circular plasmid of 6000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 1, fragments with both = 3
double digest in lane 3, fragments with both = 3
double digest in lane 3, fragments with both = 2
double digest in lane 1, fragments with both = 2
145.
The graph shows the temperature profile of two PCR cycles, starting with 2 DNA molecule(s). Which option is correct?
The graph shows the temperature profile of two PCR cycles, starting with 2 DNA molecule(s). Which option is correct?
step X: primer annealing, copies after 9 cycles = 514, time for 9 cycles = 2.08 min
step X: extension by Taq polymerase, copies after 9 cycles = 514, time for 9 cycles = 18.8 min
step X: extension by Taq polymerase, copies after 9 cycles = 1024, time for 9 cycles = 18.8 min
step X: primer annealing, copies after 9 cycles = 1024, time for 9 cycles = 37.5 min
146.
A linear DNA of 3000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A linear DNA of 3000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 3, fragments with both = 5
double digest in lane 2, fragments with both = 4
double digest in lane 3, fragments with both = 3
double digest in lane 3, fragments with both = 4
147.
The graph shows the temperature profile of two PCR cycles, starting with 5 DNA molecule(s). Which option is correct?
The graph shows the temperature profile of two PCR cycles, starting with 5 DNA molecule(s). Which option is correct?
step X: primer annealing, copies after 5 cycles = 160, time for 5 cycles = 1.67 min
step X: primer annealing, copies after 5 cycles = 160, time for 5 cycles = 8.33 min
step X: primer annealing, copies after 5 cycles = 160, time for 5 cycles = 13.3 min
step X: denaturation, copies after 5 cycles = 160, time for 5 cycles = 8.33 min
148.
A linear DNA of 8000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A linear DNA of 8000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 3, fragments with both = 5
double digest in lane 2, fragments with both = 5
double digest in lane 2, fragments with both = 3
double digest in lane 1, fragments with both = 4
149.
The graph shows the temperature profile of two PCR cycles, starting with 3 DNA molecule(s). Which option is correct?
The graph shows the temperature profile of two PCR cycles, starting with 3 DNA molecule(s). Which option is correct?
step X: denaturation, copies after 4 cycles = 48, time for 4 cycles = 2.5 min
step X: extension by Taq polymerase, copies after 4 cycles = 24, time for 4 cycles = 2.5 min
step X: extension by Taq polymerase, copies after 4 cycles = 48, time for 4 cycles = 10 min
step X: extension by Taq polymerase, copies after 4 cycles = 48, time for 4 cycles = 20 min
150.
A linear DNA of 5000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A linear DNA of 5000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 3, fragments with both = 4
double digest in lane 3, fragments with both = 3
double digest in lane 2, fragments with both = 3
double digest in lane 2, fragments with both = 4
151.
A linear DNA of 4000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A linear DNA of 4000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 3, fragments with both = 4
double digest in lane 2, fragments with both = 4
double digest in lane 3, fragments with both = 5
double digest in lane 2, fragments with both = 5
152.
A linear DNA of 8000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A linear DNA of 8000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 2, fragments with both = 4
double digest in lane 2, fragments with both = 5
double digest in lane 3, fragments with both = 4
double digest in lane 1, fragments with both = 3
153.
The graph shows the temperature profile of two PCR cycles, starting with 3 DNA molecule(s). Which option is correct?
The graph shows the temperature profile of two PCR cycles, starting with 3 DNA molecule(s). Which option is correct?
step X: extension by Taq polymerase, copies after 4 cycles = 19, time for 4 cycles = 9.33 min
step X: primer annealing, copies after 4 cycles = 48, time for 4 cycles = 18.7 min
step X: extension by Taq polymerase, copies after 4 cycles = 24, time for 4 cycles = 9.33 min
step X: extension by Taq polymerase, copies after 4 cycles = 48, time for 4 cycles = 9.33 min
154.
A linear DNA of 5000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A linear DNA of 5000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 3, fragments with both = 4
double digest in lane 3, fragments with both = 5
double digest in lane 1, fragments with both = 4
double digest in lane 2, fragments with both = 3
155.
A linear DNA of 4000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A linear DNA of 4000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 3, fragments with both = 6
double digest in lane 1, fragments with both = 6
double digest in lane 2, fragments with both = 5
double digest in lane 2, fragments with both = 4
156.
A circular plasmid of 8000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A circular plasmid of 8000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 2, fragments with both = 3
double digest in lane 1, fragments with both = 4
double digest in lane 1, fragments with both = 2
double digest in lane 3, fragments with both = 4
157.
The graph shows the temperature profile of two PCR cycles, starting with 1 DNA molecule(s). Which option is correct?
The graph shows the temperature profile of two PCR cycles, starting with 1 DNA molecule(s). Which option is correct?
step X: denaturation, copies after 12 cycles = 24, time for 12 cycles = 44 min
step X: extension by Taq polymerase, copies after 12 cycles = 4096, time for 12 cycles = 22 min
step X: primer annealing, copies after 12 cycles = 4097, time for 12 cycles = 22 min
step X: denaturation, copies after 12 cycles = 4096, time for 12 cycles = 22 min
158.
A circular plasmid of 3000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A circular plasmid of 3000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 1, fragments with both = 2
double digest in lane 3, fragments with both = 2
double digest in lane 1, fragments with both = 4
double digest in lane 3, fragments with both = 3
159.
A linear DNA of 5000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A linear DNA of 5000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 2, fragments with both = 4
double digest in lane 1, fragments with both = 5
double digest in lane 3, fragments with both = 3
double digest in lane 2, fragments with both = 5
160.
A circular plasmid of 5000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A circular plasmid of 5000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 2, fragments with both = 2
double digest in lane 1, fragments with both = 2
double digest in lane 1, fragments with both = 3
double digest in lane 3, fragments with both = 3
161.
A circular plasmid of 4000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A circular plasmid of 4000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 2, fragments with both = 4
double digest in lane 1, fragments with both = 3
double digest in lane 3, fragments with both = 3
double digest in lane 3, fragments with both = 2
162.
A linear DNA of 4000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A linear DNA of 4000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 1, fragments with both = 5
double digest in lane 2, fragments with both = 5
double digest in lane 1, fragments with both = 4
double digest in lane 3, fragments with both = 4
163.
A linear DNA of 4000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A linear DNA of 4000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 1, fragments with both = 5
double digest in lane 3, fragments with both = 4
double digest in lane 1, fragments with both = 4
double digest in lane 3, fragments with both = 3
164.
A circular plasmid of 6000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A circular plasmid of 6000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 2, fragments with both = 2
double digest in lane 1, fragments with both = 3
double digest in lane 1, fragments with both = 4
double digest in lane 3, fragments with both = 2
165.
The graph shows the temperature profile of two PCR cycles, starting with 5 DNA molecule(s). Which option is correct?
The graph shows the temperature profile of two PCR cycles, starting with 5 DNA molecule(s). Which option is correct?
step X: primer annealing, copies after 4 cycles = 21, time for 4 cycles = 9 min
step X: denaturation, copies after 4 cycles = 80, time for 4 cycles = 9 min
step X: denaturation, copies after 4 cycles = 21, time for 4 cycles = 9 min
step X: extension by Taq polymerase, copies after 4 cycles = 40, time for 4 cycles = 2.25 min
166.
A linear DNA of 4000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A linear DNA of 4000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 2, fragments with both = 4
double digest in lane 3, fragments with both = 3
double digest in lane 1, fragments with both = 3
double digest in lane 3, fragments with both = 4
167.
A circular plasmid of 5000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A circular plasmid of 5000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 3, fragments with both = 4
double digest in lane 2, fragments with both = 3
double digest in lane 2, fragments with both = 5
double digest in lane 2, fragments with both = 4
168.
The graph shows the temperature profile of two PCR cycles, starting with 10 DNA molecule(s). Which option is correct?
The graph shows the temperature profile of two PCR cycles, starting with 10 DNA molecule(s). Which option is correct?
step X: extension by Taq polymerase, copies after 10 cycles = 10240, time for 10 cycles = 15 min
step X: primer annealing, copies after 10 cycles = 5120, time for 10 cycles = 25 min
step X: primer annealing, copies after 10 cycles = 10240, time for 10 cycles = 25 min
step X: denaturation, copies after 10 cycles = 200, time for 10 cycles = 30 min
169.
A linear DNA of 4000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A linear DNA of 4000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 1, fragments with both = 3
double digest in lane 2, fragments with both = 3
double digest in lane 3, fragments with both = 5
double digest in lane 2, fragments with both = 4
170.
A linear DNA of 6000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A linear DNA of 6000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 1, fragments with both = 5
double digest in lane 2, fragments with both = 4
double digest in lane 2, fragments with both = 5
double digest in lane 1, fragments with both = 3
171.
The graph shows the temperature profile of two PCR cycles, starting with 2 DNA molecule(s). Which option is correct?
The graph shows the temperature profile of two PCR cycles, starting with 2 DNA molecule(s). Which option is correct?
step X: extension by Taq polymerase, copies after 11 cycles = 44, time for 11 cycles = 2.42 min
step X: denaturation, copies after 11 cycles = 4096, time for 11 cycles = 26.6 min
step X: extension by Taq polymerase, copies after 11 cycles = 2050, time for 11 cycles = 53.2 min
step X: denaturation, copies after 11 cycles = 44, time for 11 cycles = 26.6 min
172.
A linear DNA of 5000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A linear DNA of 5000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 3, fragments with both = 4
double digest in lane 1, fragments with both = 4
double digest in lane 1, fragments with both = 3
double digest in lane 1, fragments with both = 5
173.
A linear DNA of 6000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A linear DNA of 6000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 1, fragments with both = 5
double digest in lane 1, fragments with both = 4
double digest in lane 2, fragments with both = 5
double digest in lane 2, fragments with both = 6
174.
A linear DNA of 5000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A linear DNA of 5000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 3, fragments with both = 4
double digest in lane 2, fragments with both = 3
double digest in lane 1, fragments with both = 3
double digest in lane 2, fragments with both = 4
175.
The graph shows the temperature profile of two PCR cycles, starting with 2 DNA molecule(s). Which option is correct?
The graph shows the temperature profile of two PCR cycles, starting with 2 DNA molecule(s). Which option is correct?
step X: denaturation, copies after 6 cycles = 128, time for 6 cycles = 15 min
step X: extension by Taq polymerase, copies after 6 cycles = 24, time for 6 cycles = 15 min
step X: extension by Taq polymerase, copies after 6 cycles = 128, time for 6 cycles = 2.5 min
step X: primer annealing, copies after 6 cycles = 64, time for 6 cycles = 30 min
176.
A linear DNA of 5000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A linear DNA of 5000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 2, fragments with both = 4
double digest in lane 3, fragments with both = 5
double digest in lane 1, fragments with both = 5
double digest in lane 3, fragments with both = 3
177.
The graph shows the temperature profile of two PCR cycles, starting with 10 DNA molecule(s). Which option is correct?
The graph shows the temperature profile of two PCR cycles, starting with 10 DNA molecule(s). Which option is correct?
step X: denaturation, copies after 8 cycles = 266, time for 8 cycles = 29.3 min
step X: denaturation, copies after 8 cycles = 1280, time for 8 cycles = 29.3 min
step X: primer annealing, copies after 8 cycles = 266, time for 8 cycles = 14.7 min
step X: primer annealing, copies after 8 cycles = 2560, time for 8 cycles = 14.7 min
178.
A circular plasmid of 4000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A circular plasmid of 4000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 1, fragments with both = 3
double digest in lane 2, fragments with both = 4
double digest in lane 3, fragments with both = 4
double digest in lane 1, fragments with both = 2
179.
A linear DNA of 4000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A linear DNA of 4000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 1, fragments with both = 4
double digest in lane 1, fragments with both = 5
double digest in lane 2, fragments with both = 4
double digest in lane 3, fragments with both = 4
180.
The graph shows the temperature profile of two PCR cycles, starting with 5 DNA molecule(s). Which option is correct?
The graph shows the temperature profile of two PCR cycles, starting with 5 DNA molecule(s). Which option is correct?
step X: denaturation, copies after 8 cycles = 1280, time for 8 cycles = 46.7 min
step X: denaturation, copies after 8 cycles = 1280, time for 8 cycles = 23.3 min
step X: denaturation, copies after 8 cycles = 80, time for 8 cycles = 23.3 min
step X: extension by Taq polymerase, copies after 8 cycles = 261, time for 8 cycles = 23.3 min
181.
A circular plasmid of 3000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A circular plasmid of 3000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 1, fragments with both = 5
double digest in lane 2, fragments with both = 3
double digest in lane 1, fragments with both = 4
double digest in lane 3, fragments with both = 4
182.
A linear DNA of 5000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A linear DNA of 5000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 2, fragments with both = 5
double digest in lane 3, fragments with both = 4
double digest in lane 1, fragments with both = 5
double digest in lane 1, fragments with both = 4
183.
A circular plasmid of 3000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A circular plasmid of 3000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 3, fragments with both = 4
double digest in lane 1, fragments with both = 4
double digest in lane 1, fragments with both = 3
double digest in lane 3, fragments with both = 3
184.
The graph shows the temperature profile of two PCR cycles, starting with 3 DNA molecule(s). Which option is correct?
The graph shows the temperature profile of two PCR cycles, starting with 3 DNA molecule(s). Which option is correct?
step X: primer annealing, copies after 7 cycles = 42, time for 7 cycles = 23.3 min
step X: extension by Taq polymerase, copies after 7 cycles = 192, time for 7 cycles = 23.3 min
step X: extension by Taq polymerase, copies after 7 cycles = 384, time for 7 cycles = 11.7 min
step X: primer annealing, copies after 7 cycles = 42, time for 7 cycles = 18.7 min
185.
A linear DNA of 5000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A linear DNA of 5000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 1, fragments with both = 3
double digest in lane 2, fragments with both = 5
double digest in lane 2, fragments with both = 4
double digest in lane 3, fragments with both = 3
186.
The graph shows the temperature profile of two PCR cycles, starting with 2 DNA molecule(s). Which option is correct?
The graph shows the temperature profile of two PCR cycles, starting with 2 DNA molecule(s). Which option is correct?
step X: denaturation, copies after 11 cycles = 4096, time for 11 cycles = 33.9 min
step X: primer annealing, copies after 11 cycles = 4096, time for 11 cycles = 22.9 min
step X: denaturation, copies after 11 cycles = 4096, time for 11 cycles = 22.9 min
step X: extension by Taq polymerase, copies after 11 cycles = 44, time for 11 cycles = 33.9 min
187.
The graph shows the temperature profile of two PCR cycles, starting with 3 DNA molecule(s). Which option is correct?
The graph shows the temperature profile of two PCR cycles, starting with 3 DNA molecule(s). Which option is correct?
step X: extension by Taq polymerase, copies after 12 cycles = 12288, time for 12 cycles = 22 min
step X: denaturation, copies after 12 cycles = 12288, time for 12 cycles = 44 min
step X: primer annealing, copies after 12 cycles = 6144, time for 12 cycles = 44 min
step X: extension by Taq polymerase, copies after 12 cycles = 4099, time for 12 cycles = 1.83 min
188.
A linear DNA of 4000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A linear DNA of 4000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 3, fragments with both = 6
double digest in lane 1, fragments with both = 5
double digest in lane 3, fragments with both = 5
double digest in lane 3, fragments with both = 4
189.
A linear DNA of 4000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A linear DNA of 4000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 3, fragments with both = 6
double digest in lane 2, fragments with both = 5
double digest in lane 3, fragments with both = 5
double digest in lane 1, fragments with both = 5
190.
A linear DNA of 3000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A linear DNA of 3000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 3, fragments with both = 3
double digest in lane 2, fragments with both = 4
double digest in lane 1, fragments with both = 4
double digest in lane 1, fragments with both = 3
191.
A linear DNA of 5000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A linear DNA of 5000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 3, fragments with both = 2
double digest in lane 3, fragments with both = 3
double digest in lane 2, fragments with both = 2
double digest in lane 1, fragments with both = 3
192.
A linear DNA of 6000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A linear DNA of 6000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 1, fragments with both = 6
double digest in lane 1, fragments with both = 5
double digest in lane 2, fragments with both = 6
double digest in lane 3, fragments with both = 6
193.
The graph shows the temperature profile of two PCR cycles, starting with 5 DNA molecule(s). Which option is correct?
The graph shows the temperature profile of two PCR cycles, starting with 5 DNA molecule(s). Which option is correct?
step X: extension by Taq polymerase, copies after 9 cycles = 90, time for 9 cycles = 16.5 min
step X: denaturation, copies after 9 cycles = 2560, time for 9 cycles = 16.5 min
step X: denaturation, copies after 9 cycles = 1280, time for 9 cycles = 16.5 min
step X: extension by Taq polymerase, copies after 9 cycles = 517, time for 9 cycles = 25.5 min
194.
A linear DNA of 3000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A linear DNA of 3000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 3, fragments with both = 5
double digest in lane 1, fragments with both = 4
double digest in lane 2, fragments with both = 6
double digest in lane 1, fragments with both = 5
195.
A circular plasmid of 3000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A circular plasmid of 3000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 2, fragments with both = 4
double digest in lane 3, fragments with both = 4
double digest in lane 1, fragments with both = 3
double digest in lane 2, fragments with both = 3
196.
A linear DNA of 4000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A linear DNA of 4000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 3, fragments with both = 3
double digest in lane 1, fragments with both = 5
double digest in lane 2, fragments with both = 3
double digest in lane 1, fragments with both = 4
197.
A linear DNA of 6000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A linear DNA of 6000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 1, fragments with both = 3
double digest in lane 2, fragments with both = 3
double digest in lane 3, fragments with both = 4
double digest in lane 3, fragments with both = 3
198.
The graph shows the temperature profile of two PCR cycles, starting with 1 DNA molecule(s). Which option is correct?
The graph shows the temperature profile of two PCR cycles, starting with 1 DNA molecule(s). Which option is correct?
step X: extension by Taq polymerase, copies after 5 cycles = 32, time for 5 cycles = 7.5 min
step X: primer annealing, copies after 5 cycles = 32, time for 5 cycles = 1.5 min
step X: primer annealing, copies after 5 cycles = 10, time for 5 cycles = 1.5 min
step X: extension by Taq polymerase, copies after 5 cycles = 32, time for 5 cycles = 1.5 min
199.
A linear DNA of 6000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A linear DNA of 6000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 2, fragments with both = 5
double digest in lane 1, fragments with both = 5
double digest in lane 1, fragments with both = 4
double digest in lane 3, fragments with both = 4
200.
A linear DNA of 4000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A linear DNA of 4000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 3, fragments with both = 4
double digest in lane 1, fragments with both = 4
double digest in lane 2, fragments with both = 4
double digest in lane 1, fragments with both = 3
201.
A circular plasmid of 6000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A circular plasmid of 6000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 2, fragments with both = 3
double digest in lane 1, fragments with both = 2
double digest in lane 1, fragments with both = 3
double digest in lane 3, fragments with both = 2
202.
A linear DNA of 5000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A linear DNA of 5000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 1, fragments with both = 4
double digest in lane 1, fragments with both = 3
double digest in lane 2, fragments with both = 2
double digest in lane 1, fragments with both = 2
203.
A linear DNA of 4000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A linear DNA of 4000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 1, fragments with both = 4
double digest in lane 3, fragments with both = 4
double digest in lane 3, fragments with both = 5
double digest in lane 2, fragments with both = 5
204.
A circular plasmid of 6000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A circular plasmid of 6000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 1, fragments with both = 4
double digest in lane 2, fragments with both = 3
double digest in lane 1, fragments with both = 3
double digest in lane 3, fragments with both = 4
205.
A linear DNA of 4000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A linear DNA of 4000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 1, fragments with both = 3
double digest in lane 3, fragments with both = 4
double digest in lane 3, fragments with both = 5
double digest in lane 2, fragments with both = 4
206.
A linear DNA of 3000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A linear DNA of 3000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 2, fragments with both = 6
double digest in lane 2, fragments with both = 5
double digest in lane 1, fragments with both = 6
double digest in lane 3, fragments with both = 6
207.
A linear DNA of 4000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A linear DNA of 4000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 2, fragments with both = 5
double digest in lane 3, fragments with both = 5
double digest in lane 3, fragments with both = 4
double digest in lane 1, fragments with both = 6
208.
A linear DNA of 3000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A linear DNA of 3000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 3, fragments with both = 6
double digest in lane 3, fragments with both = 4
double digest in lane 1, fragments with both = 6
double digest in lane 1, fragments with both = 5
209.
A linear DNA of 3000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A linear DNA of 3000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 2, fragments with both = 2
double digest in lane 1, fragments with both = 4
double digest in lane 2, fragments with both = 3
double digest in lane 3, fragments with both = 2
210.
A linear DNA of 8000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A linear DNA of 8000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 1, fragments with both = 3
double digest in lane 1, fragments with both = 5
double digest in lane 2, fragments with both = 4
double digest in lane 3, fragments with both = 4
211.
The graph shows the temperature profile of two PCR cycles, starting with 3 DNA molecule(s). Which option is correct?
The graph shows the temperature profile of two PCR cycles, starting with 3 DNA molecule(s). Which option is correct?
step X: primer annealing, copies after 4 cycles = 19, time for 4 cycles = 7.33 min
step X: extension by Taq polymerase, copies after 4 cycles = 48, time for 4 cycles = 7.33 min
step X: denaturation, copies after 4 cycles = 24, time for 4 cycles = 14.7 min
step X: extension by Taq polymerase, copies after 4 cycles = 19, time for 4 cycles = 7.33 min
212.
A linear DNA of 4000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A linear DNA of 4000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 3, fragments with both = 3
double digest in lane 1, fragments with both = 4
double digest in lane 1, fragments with both = 3
double digest in lane 3, fragments with both = 4
213.
The graph shows the temperature profile of two PCR cycles, starting with 1 DNA molecule(s). Which option is correct?
The graph shows the temperature profile of two PCR cycles, starting with 1 DNA molecule(s). Which option is correct?
step X: primer annealing, copies after 7 cycles = 64, time for 7 cycles = 16.9 min
step X: primer annealing, copies after 7 cycles = 128, time for 7 cycles = 2.42 min
step X: primer annealing, copies after 7 cycles = 128, time for 7 cycles = 16.9 min
step X: extension by Taq polymerase, copies after 7 cycles = 129, time for 7 cycles = 16.9 min
214.
A circular plasmid of 6000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A circular plasmid of 6000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 3, fragments with both = 4
double digest in lane 3, fragments with both = 2
double digest in lane 1, fragments with both = 3
double digest in lane 3, fragments with both = 3
215.
A linear DNA of 8000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A linear DNA of 8000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 1, fragments with both = 4
double digest in lane 3, fragments with both = 3
double digest in lane 3, fragments with both = 4
double digest in lane 2, fragments with both = 3
216.
A linear DNA of 4000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A linear DNA of 4000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 1, fragments with both = 3
double digest in lane 1, fragments with both = 2
double digest in lane 2, fragments with both = 3
double digest in lane 3, fragments with both = 3
217.
The graph shows the temperature profile of two PCR cycles, starting with 3 DNA molecule(s). Which option is correct?
The graph shows the temperature profile of two PCR cycles, starting with 3 DNA molecule(s). Which option is correct?
step X: extension by Taq polymerase, copies after 12 cycles = 4099, time for 12 cycles = 2.17 min
step X: primer annealing, copies after 12 cycles = 4099, time for 12 cycles = 26 min
step X: denaturation, copies after 12 cycles = 12288, time for 12 cycles = 26 min
step X: denaturation, copies after 12 cycles = 6144, time for 12 cycles = 26 min
218.
A circular plasmid of 3000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A circular plasmid of 3000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 1, fragments with both = 4
double digest in lane 3, fragments with both = 4
double digest in lane 3, fragments with both = 3
double digest in lane 2, fragments with both = 3
219.
A circular plasmid of 8000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A circular plasmid of 8000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 2, fragments with both = 4
double digest in lane 1, fragments with both = 3
double digest in lane 1, fragments with both = 2
double digest in lane 3, fragments with both = 2
220.
A circular plasmid of 5000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A circular plasmid of 5000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 3, fragments with both = 5
double digest in lane 2, fragments with both = 4
double digest in lane 1, fragments with both = 3
double digest in lane 2, fragments with both = 5
221.
A linear DNA of 8000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A linear DNA of 8000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 1, fragments with both = 4
double digest in lane 2, fragments with both = 5
double digest in lane 3, fragments with both = 3
double digest in lane 1, fragments with both = 5
222.
A linear DNA of 6000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A linear DNA of 6000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 2, fragments with both = 5
double digest in lane 1, fragments with both = 4
double digest in lane 1, fragments with both = 3
double digest in lane 3, fragments with both = 4
223.
The graph shows the temperature profile of two PCR cycles, starting with 1 DNA molecule(s). Which option is correct?
The graph shows the temperature profile of two PCR cycles, starting with 1 DNA molecule(s). Which option is correct?
step X: extension by Taq polymerase, copies after 9 cycles = 256, time for 9 cycles = 30 min
step X: primer annealing, copies after 9 cycles = 18, time for 9 cycles = 30 min
step X: extension by Taq polymerase, copies after 9 cycles = 513, time for 9 cycles = 21 min
step X: extension by Taq polymerase, copies after 9 cycles = 512, time for 9 cycles = 21 min
224.
A linear DNA of 3000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A linear DNA of 3000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 1, fragments with both = 6
double digest in lane 1, fragments with both = 5
double digest in lane 2, fragments with both = 5
double digest in lane 3, fragments with both = 5
225.
A linear DNA of 6000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A linear DNA of 6000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 2, fragments with both = 2
double digest in lane 1, fragments with both = 3
double digest in lane 3, fragments with both = 3
double digest in lane 1, fragments with both = 2
226.
A circular plasmid of 5000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A circular plasmid of 5000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 2, fragments with both = 3
double digest in lane 1, fragments with both = 4
double digest in lane 1, fragments with both = 2
double digest in lane 1, fragments with both = 3
227.
A circular plasmid of 3000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A circular plasmid of 3000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 2, fragments with both = 4
double digest in lane 2, fragments with both = 5
double digest in lane 1, fragments with both = 4
double digest in lane 3, fragments with both = 4
228.
A circular plasmid of 3000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A circular plasmid of 3000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 1, fragments with both = 3
double digest in lane 2, fragments with both = 4
double digest in lane 2, fragments with both = 3
double digest in lane 3, fragments with both = 3
229.
A circular plasmid of 3000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A circular plasmid of 3000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 2, fragments with both = 3
double digest in lane 1, fragments with both = 2
double digest in lane 1, fragments with both = 3
double digest in lane 1, fragments with both = 4
230.
A circular plasmid of 8000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A circular plasmid of 8000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 3, fragments with both = 3
double digest in lane 3, fragments with both = 2
double digest in lane 1, fragments with both = 3
double digest in lane 2, fragments with both = 4
231.
A circular plasmid of 5000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A circular plasmid of 5000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 1, fragments with both = 5
double digest in lane 3, fragments with both = 5
double digest in lane 3, fragments with both = 3
double digest in lane 3, fragments with both = 4
232.
A linear DNA of 4000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A linear DNA of 4000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 1, fragments with both = 4
double digest in lane 2, fragments with both = 3
double digest in lane 2, fragments with both = 5
double digest in lane 3, fragments with both = 4
233.
A linear DNA of 8000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A linear DNA of 8000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 3, fragments with both = 4
double digest in lane 1, fragments with both = 3
double digest in lane 1, fragments with both = 4
double digest in lane 2, fragments with both = 4
234.
A linear DNA of 6000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A linear DNA of 6000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 3, fragments with both = 4
double digest in lane 1, fragments with both = 4
double digest in lane 3, fragments with both = 3
double digest in lane 1, fragments with both = 5
235.
A circular plasmid of 5000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A circular plasmid of 5000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 2, fragments with both = 3
double digest in lane 3, fragments with both = 4
double digest in lane 3, fragments with both = 3
double digest in lane 2, fragments with both = 4
236.
The graph shows the temperature profile of two PCR cycles, starting with 10 DNA molecule(s). Which option is correct?
The graph shows the temperature profile of two PCR cycles, starting with 10 DNA molecule(s). Which option is correct?
step X: extension by Taq polymerase, copies after 11 cycles = 2058, time for 11 cycles = 23.8 min
step X: denaturation, copies after 11 cycles = 20480, time for 11 cycles = 34.8 min
step X: denaturation, copies after 11 cycles = 20480, time for 11 cycles = 23.8 min
step X: primer annealing, copies after 11 cycles = 20480, time for 11 cycles = 23.8 min
237.
A circular plasmid of 8000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A circular plasmid of 8000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 3, fragments with both = 3
double digest in lane 1, fragments with both = 4
double digest in lane 1, fragments with both = 3
double digest in lane 3, fragments with both = 2
238.
The graph shows the temperature profile of two PCR cycles, starting with 10 DNA molecule(s). Which option is correct?
The graph shows the temperature profile of two PCR cycles, starting with 10 DNA molecule(s). Which option is correct?
step X: denaturation, copies after 12 cycles = 4106, time for 12 cycles = 24 min
step X: primer annealing, copies after 12 cycles = 40960, time for 12 cycles = 48 min
step X: denaturation, copies after 12 cycles = 40960, time for 12 cycles = 24 min
step X: primer annealing, copies after 12 cycles = 40960, time for 12 cycles = 2 min
239.
A linear DNA of 8000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A linear DNA of 8000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 2, fragments with both = 3
double digest in lane 1, fragments with both = 3
double digest in lane 2, fragments with both = 4
double digest in lane 1, fragments with both = 5
240.
The graph shows the temperature profile of two PCR cycles, starting with 1 DNA molecule(s). Which option is correct?
The graph shows the temperature profile of two PCR cycles, starting with 1 DNA molecule(s). Which option is correct?
step X: denaturation, copies after 12 cycles = 24, time for 12 cycles = 36 min
step X: denaturation, copies after 12 cycles = 4097, time for 12 cycles = 2 min
step X: primer annealing, copies after 12 cycles = 24, time for 12 cycles = 24 min
step X: primer annealing, copies after 12 cycles = 4096, time for 12 cycles = 24 min
241.
A circular plasmid of 3000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A circular plasmid of 3000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 2, fragments with both = 4
double digest in lane 1, fragments with both = 4
double digest in lane 3, fragments with both = 5
double digest in lane 1, fragments with both = 5
242.
The graph shows the temperature profile of two PCR cycles, starting with 10 DNA molecule(s). Which option is correct?
The graph shows the temperature profile of two PCR cycles, starting with 10 DNA molecule(s). Which option is correct?
step X: extension by Taq polymerase, copies after 6 cycles = 640, time for 6 cycles = 10 min
step X: extension by Taq polymerase, copies after 6 cycles = 320, time for 6 cycles = 1.67 min
step X: primer annealing, copies after 6 cycles = 74, time for 6 cycles = 16 min
step X: denaturation, copies after 6 cycles = 640, time for 6 cycles = 10 min
243.
A linear DNA of 8000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A linear DNA of 8000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 2, fragments with both = 4
double digest in lane 2, fragments with both = 3
double digest in lane 1, fragments with both = 3
double digest in lane 3, fragments with both = 2
244.
A circular plasmid of 4000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A circular plasmid of 4000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 3, fragments with both = 3
double digest in lane 1, fragments with both = 2
double digest in lane 2, fragments with both = 3
double digest in lane 2, fragments with both = 4
245.
A linear DNA of 5000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A linear DNA of 5000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 2, fragments with both = 5
double digest in lane 2, fragments with both = 3
double digest in lane 3, fragments with both = 5
double digest in lane 1, fragments with both = 4
246.
The graph shows the temperature profile of two PCR cycles, starting with 10 DNA molecule(s). Which option is correct?
The graph shows the temperature profile of two PCR cycles, starting with 10 DNA molecule(s). Which option is correct?
step X: denaturation, copies after 7 cycles = 1280, time for 7 cycles = 11.7 min
step X: primer annealing, copies after 7 cycles = 640, time for 7 cycles = 11.7 min
step X: primer annealing, copies after 7 cycles = 138, time for 7 cycles = 1.67 min
step X: extension by Taq polymerase, copies after 7 cycles = 640, time for 7 cycles = 1.67 min
247.
A circular plasmid of 8000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A circular plasmid of 8000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 2, fragments with both = 3
double digest in lane 3, fragments with both = 5
double digest in lane 2, fragments with both = 4
double digest in lane 3, fragments with both = 3
248.
The graph shows the temperature profile of two PCR cycles, starting with 1 DNA molecule(s). Which option is correct?
The graph shows the temperature profile of two PCR cycles, starting with 1 DNA molecule(s). Which option is correct?
step X: extension by Taq polymerase, copies after 11 cycles = 1024, time for 11 cycles = 24.8 min
step X: denaturation, copies after 11 cycles = 2048, time for 11 cycles = 24.8 min
step X: primer annealing, copies after 11 cycles = 22, time for 11 cycles = 2.25 min
step X: primer annealing, copies after 11 cycles = 2049, time for 11 cycles = 24.8 min
249.
A linear DNA of 8000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A linear DNA of 8000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 1, fragments with both = 4
double digest in lane 2, fragments with both = 3
double digest in lane 1, fragments with both = 5
double digest in lane 2, fragments with both = 4
250.
The graph shows the temperature profile of two PCR cycles, starting with 1 DNA molecule(s). Which option is correct?
The graph shows the temperature profile of two PCR cycles, starting with 1 DNA molecule(s). Which option is correct?
step X: denaturation, copies after 11 cycles = 2048, time for 11 cycles = 33 min
step X: primer annealing, copies after 11 cycles = 2048, time for 11 cycles = 66 min
step X: extension by Taq polymerase, copies after 11 cycles = 1024, time for 11 cycles = 66 min
step X: denaturation, copies after 11 cycles = 2049, time for 11 cycles = 44 min
251.
A linear DNA of 8000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A linear DNA of 8000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 2, fragments with both = 4
double digest in lane 2, fragments with both = 5
double digest in lane 1, fragments with both = 3
double digest in lane 1, fragments with both = 5
252.
A linear DNA of 5000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A linear DNA of 5000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 1, fragments with both = 6
double digest in lane 1, fragments with both = 4
double digest in lane 2, fragments with both = 4
double digest in lane 2, fragments with both = 5
253.
A linear DNA of 6000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A linear DNA of 6000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 2, fragments with both = 3
double digest in lane 3, fragments with both = 3
double digest in lane 3, fragments with both = 4
double digest in lane 1, fragments with both = 2
254.
A linear DNA of 5000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A linear DNA of 5000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 3, fragments with both = 4
double digest in lane 2, fragments with both = 4
double digest in lane 1, fragments with both = 4
double digest in lane 1, fragments with both = 5
255.
A circular plasmid of 6000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A circular plasmid of 6000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 3, fragments with both = 4
double digest in lane 2, fragments with both = 4
double digest in lane 3, fragments with both = 3
double digest in lane 2, fragments with both = 3
256.
A circular plasmid of 8000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A circular plasmid of 8000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 1, fragments with both = 5
double digest in lane 2, fragments with both = 3
double digest in lane 3, fragments with both = 4
double digest in lane 2, fragments with both = 5
257.
A circular plasmid of 5000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A circular plasmid of 5000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 3, fragments with both = 4
double digest in lane 2, fragments with both = 5
double digest in lane 2, fragments with both = 3
double digest in lane 1, fragments with both = 4
258.
A linear DNA of 5000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A linear DNA of 5000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 1, fragments with both = 4
double digest in lane 2, fragments with both = 3
double digest in lane 2, fragments with both = 5
double digest in lane 3, fragments with both = 3
259.
The graph shows the temperature profile of two PCR cycles, starting with 10 DNA molecule(s). Which option is correct?
The graph shows the temperature profile of two PCR cycles, starting with 10 DNA molecule(s). Which option is correct?
step X: denaturation, copies after 10 cycles = 1034, time for 10 cycles = 25 min
step X: denaturation, copies after 10 cycles = 10240, time for 10 cycles = 15 min
step X: primer annealing, copies after 10 cycles = 200, time for 10 cycles = 25 min
step X: extension by Taq polymerase, copies after 10 cycles = 1034, time for 10 cycles = 25 min
260.
A linear DNA of 3000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A linear DNA of 3000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 3, fragments with both = 4
double digest in lane 1, fragments with both = 4
double digest in lane 2, fragments with both = 5
double digest in lane 1, fragments with both = 3
261.
A linear DNA of 8000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A linear DNA of 8000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 1, fragments with both = 5
double digest in lane 1, fragments with both = 4
double digest in lane 3, fragments with both = 4
double digest in lane 2, fragments with both = 4
262.
A linear DNA of 5000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A linear DNA of 5000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 1, fragments with both = 5
double digest in lane 3, fragments with both = 6
double digest in lane 3, fragments with both = 4
double digest in lane 2, fragments with both = 4
263.
A linear DNA of 3000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A linear DNA of 3000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 1, fragments with both = 5
double digest in lane 2, fragments with both = 5
double digest in lane 3, fragments with both = 4
double digest in lane 3, fragments with both = 5
264.
A circular plasmid of 8000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A circular plasmid of 8000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 1, fragments with both = 4
double digest in lane 3, fragments with both = 5
double digest in lane 2, fragments with both = 5
double digest in lane 2, fragments with both = 4
265.
The graph shows the temperature profile of two PCR cycles, starting with 2 DNA molecule(s). Which option is correct?
The graph shows the temperature profile of two PCR cycles, starting with 2 DNA molecule(s). Which option is correct?
step X: primer annealing, copies after 7 cycles = 28, time for 7 cycles = 15.8 min
step X: extension by Taq polymerase, copies after 7 cycles = 28, time for 7 cycles = 22.8 min
step X: primer annealing, copies after 7 cycles = 256, time for 7 cycles = 15.8 min
step X: denaturation, copies after 7 cycles = 256, time for 7 cycles = 22.8 min
266.
A linear DNA of 6000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A linear DNA of 6000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 2, fragments with both = 5
double digest in lane 1, fragments with both = 4
double digest in lane 1, fragments with both = 5
double digest in lane 2, fragments with both = 4
267.
A linear DNA of 6000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A linear DNA of 6000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 3, fragments with both = 3
double digest in lane 2, fragments with both = 4
double digest in lane 1, fragments with both = 4
double digest in lane 2, fragments with both = 3
268.
A linear DNA of 3000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A linear DNA of 3000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 1, fragments with both = 4
double digest in lane 2, fragments with both = 4
double digest in lane 3, fragments with both = 5
double digest in lane 1, fragments with both = 3
269.
A circular plasmid of 6000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A circular plasmid of 6000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 2, fragments with both = 2
double digest in lane 2, fragments with both = 4
double digest in lane 3, fragments with both = 3
double digest in lane 1, fragments with both = 2
270.
A circular plasmid of 8000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A circular plasmid of 8000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 3, fragments with both = 3
double digest in lane 2, fragments with both = 4
double digest in lane 3, fragments with both = 5
double digest in lane 2, fragments with both = 5
271.
A linear DNA of 5000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A linear DNA of 5000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 1, fragments with both = 5
double digest in lane 1, fragments with both = 3
double digest in lane 3, fragments with both = 3
double digest in lane 1, fragments with both = 4
272.
A linear DNA of 8000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A linear DNA of 8000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 3, fragments with both = 4
double digest in lane 2, fragments with both = 6
double digest in lane 3, fragments with both = 6
double digest in lane 3, fragments with both = 5
273.
A linear DNA of 6000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A linear DNA of 6000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 2, fragments with both = 3
double digest in lane 2, fragments with both = 4
double digest in lane 1, fragments with both = 4
double digest in lane 1, fragments with both = 3
274.
The graph shows the temperature profile of two PCR cycles, starting with 3 DNA molecule(s). Which option is correct?
The graph shows the temperature profile of two PCR cycles, starting with 3 DNA molecule(s). Which option is correct?
step X: primer annealing, copies after 9 cycles = 1536, time for 9 cycles = 25.5 min
step X: extension by Taq polymerase, copies after 9 cycles = 1536, time for 9 cycles = 33 min
step X: denaturation, copies after 9 cycles = 1536, time for 9 cycles = 16.5 min
step X: primer annealing, copies after 9 cycles = 768, time for 9 cycles = 16.5 min
275.
A linear DNA of 5000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A linear DNA of 5000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 2, fragments with both = 5
double digest in lane 2, fragments with both = 4
double digest in lane 2, fragments with both = 6
double digest in lane 3, fragments with both = 5
276.
A circular plasmid of 6000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A circular plasmid of 6000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 3, fragments with both = 4
double digest in lane 2, fragments with both = 5
double digest in lane 3, fragments with both = 5
double digest in lane 2, fragments with both = 4
277.
A linear DNA of 6000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A linear DNA of 6000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 2, fragments with both = 5
double digest in lane 2, fragments with both = 4
double digest in lane 3, fragments with both = 4
double digest in lane 3, fragments with both = 5
278.
A linear DNA of 3000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A linear DNA of 3000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 1, fragments with both = 3
double digest in lane 3, fragments with both = 3
double digest in lane 3, fragments with both = 4
double digest in lane 2, fragments with both = 4
279.
The graph shows the temperature profile of two PCR cycles, starting with 10 DNA molecule(s). Which option is correct?
The graph shows the temperature profile of two PCR cycles, starting with 10 DNA molecule(s). Which option is correct?
step X: denaturation, copies after 9 cycles = 5120, time for 9 cycles = 30 min
step X: denaturation, copies after 9 cycles = 5120, time for 9 cycles = 21 min
step X: denaturation, copies after 9 cycles = 180, time for 9 cycles = 42 min
step X: primer annealing, copies after 9 cycles = 5120, time for 9 cycles = 21 min
280.
A circular plasmid of 4000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A circular plasmid of 4000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 1, fragments with both = 2
double digest in lane 3, fragments with both = 2
double digest in lane 3, fragments with both = 4
double digest in lane 1, fragments with both = 3
281.
The graph shows the temperature profile of two PCR cycles, starting with 5 DNA molecule(s). Which option is correct?
The graph shows the temperature profile of two PCR cycles, starting with 5 DNA molecule(s). Which option is correct?
step X: extension by Taq polymerase, copies after 11 cycles = 10240, time for 11 cycles = 18.3 min
step X: denaturation, copies after 11 cycles = 5120, time for 11 cycles = 18.3 min
step X: denaturation, copies after 11 cycles = 10240, time for 11 cycles = 1.67 min
step X: primer annealing, copies after 11 cycles = 2053, time for 11 cycles = 18.3 min
282.
A linear DNA of 4000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A linear DNA of 4000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 1, fragments with both = 5
double digest in lane 3, fragments with both = 5
double digest in lane 1, fragments with both = 3
double digest in lane 1, fragments with both = 4
283.
A linear DNA of 4000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A linear DNA of 4000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 1, fragments with both = 4
double digest in lane 1, fragments with both = 2
double digest in lane 2, fragments with both = 3
double digest in lane 3, fragments with both = 3
284.
A circular plasmid of 8000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A circular plasmid of 8000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 2, fragments with both = 3
double digest in lane 3, fragments with both = 2
double digest in lane 1, fragments with both = 2
double digest in lane 3, fragments with both = 4
285.
A circular plasmid of 4000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A circular plasmid of 4000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 2, fragments with both = 4
double digest in lane 1, fragments with both = 3
double digest in lane 3, fragments with both = 3
double digest in lane 1, fragments with both = 4
286.
A linear DNA of 4000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A linear DNA of 4000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 3, fragments with both = 5
double digest in lane 1, fragments with both = 5
double digest in lane 2, fragments with both = 4
double digest in lane 1, fragments with both = 4
287.
A linear DNA of 3000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A linear DNA of 3000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 2, fragments with both = 3
double digest in lane 3, fragments with both = 3
double digest in lane 3, fragments with both = 4
double digest in lane 2, fragments with both = 4
288.
A linear DNA of 3000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A linear DNA of 3000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 3, fragments with both = 5
double digest in lane 1, fragments with both = 4
double digest in lane 2, fragments with both = 5
double digest in lane 3, fragments with both = 4
289.
A linear DNA of 4000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A linear DNA of 4000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 1, fragments with both = 4
double digest in lane 2, fragments with both = 4
double digest in lane 3, fragments with both = 5
double digest in lane 3, fragments with both = 4
290.
A linear DNA of 3000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A linear DNA of 3000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 2, fragments with both = 3
double digest in lane 3, fragments with both = 5
double digest in lane 2, fragments with both = 5
double digest in lane 1, fragments with both = 4
291.
A circular plasmid of 5000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A circular plasmid of 5000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 2, fragments with both = 2
double digest in lane 1, fragments with both = 3
double digest in lane 3, fragments with both = 2
double digest in lane 1, fragments with both = 4
292.
The graph shows the temperature profile of two PCR cycles, starting with 3 DNA molecule(s). Which option is correct?
The graph shows the temperature profile of two PCR cycles, starting with 3 DNA molecule(s). Which option is correct?
step X: primer annealing, copies after 9 cycles = 1536, time for 9 cycles = 19.5 min
step X: extension by Taq polymerase, copies after 9 cycles = 515, time for 9 cycles = 19.5 min
step X: extension by Taq polymerase, copies after 9 cycles = 1536, time for 9 cycles = 19.5 min
step X: denaturation, copies after 9 cycles = 768, time for 9 cycles = 39 min
293.
A circular plasmid of 3000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A circular plasmid of 3000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 1, fragments with both = 4
double digest in lane 1, fragments with both = 3
double digest in lane 3, fragments with both = 4
double digest in lane 2, fragments with both = 4
294.
A linear DNA of 8000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A linear DNA of 8000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 2, fragments with both = 5
double digest in lane 3, fragments with both = 5
double digest in lane 3, fragments with both = 4
double digest in lane 3, fragments with both = 6
295.
The graph shows the temperature profile of two PCR cycles, starting with 10 DNA molecule(s). Which option is correct?
The graph shows the temperature profile of two PCR cycles, starting with 10 DNA molecule(s). Which option is correct?
step X: extension by Taq polymerase, copies after 7 cycles = 1280, time for 7 cycles = 10.5 min
step X: extension by Taq polymerase, copies after 7 cycles = 1280, time for 7 cycles = 17.5 min
step X: primer annealing, copies after 7 cycles = 1280, time for 7 cycles = 10.5 min
step X: denaturation, copies after 7 cycles = 1280, time for 7 cycles = 10.5 min
296.
The graph shows the temperature profile of two PCR cycles, starting with 5 DNA molecule(s). Which option is correct?
The graph shows the temperature profile of two PCR cycles, starting with 5 DNA molecule(s). Which option is correct?
step X: extension by Taq polymerase, copies after 5 cycles = 160, time for 5 cycles = 10.4 min
step X: denaturation, copies after 5 cycles = 160, time for 5 cycles = 20.8 min
step X: primer annealing, copies after 5 cycles = 160, time for 5 cycles = 10.4 min
step X: denaturation, copies after 5 cycles = 37, time for 5 cycles = 10.4 min
297.
A linear DNA of 4000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A linear DNA of 4000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 2, fragments with both = 5
double digest in lane 2, fragments with both = 4
double digest in lane 3, fragments with both = 5
double digest in lane 1, fragments with both = 4
298.
A linear DNA of 5000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A linear DNA of 5000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 3, fragments with both = 3
double digest in lane 2, fragments with both = 3
double digest in lane 3, fragments with both = 4
double digest in lane 3, fragments with both = 5
299.
A linear DNA of 6000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A linear DNA of 6000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 1, fragments with both = 5
double digest in lane 3, fragments with both = 4
double digest in lane 1, fragments with both = 4
double digest in lane 2, fragments with both = 6
300.
A linear DNA of 5000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
A linear DNA of 5000 bp was cut with restriction enzymes A and B (sites on the map) and run on a gel (figure). Which option is correct?
double digest in lane 1, fragments with both = 3
double digest in lane 3, fragments with both = 3
double digest in lane 2, fragments with both = 3
double digest in lane 1, fragments with both = 2
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