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6.1Organisms Respond to Changes in their Environments (A Level only)

Response to Stimuli (A Level only) — AQA A Level Biology 7402 Revision Notes & Topical Questions

20 min read 27 parts Lesson 2 of 44 Unit quiz

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Survival and Plant Responses: Growth Factors and IAA — AQA A LEVEL BIOLOGY 7402 notes: Explain how plants respond to directional stimuli using growth factors, and describe the effect of IAA on shoots and roots.Detecting and responding to environmental change helps organisms survi…

  • cell elongation
  • Worked example 1
  • Worked example 2
  • Worked example 3
  • IAA (indoleacetic acid)
  • Phototropism

Section 1 of 27Why responding to the environment helps survival

Detecting and responding to environmental change helps organisms survive: it lets them avoid harmful conditions, find food and escape predators. The process is: a stimulus is detected by receptor cells, information is coordinated, and effectors produce a response. Plants as well as animals depend on this ability.

Section 2 of 27Growth factors and tropisms

Plants carry out growth responses to directional stimuli such as light and gravity. At GCSE these substances are called plant hormones, but at A Level you must always use the term growth factors. A growth response towards or away from a directional stimulus is a tropism; growth towards light is positive phototropism.

Section 3 of 27Indoleacetic acid (IAA)

IAA is a plant growth factor and belongs to the group called auxins. It is synthesised in the growing tips of roots and shoots and then moves to other regions of the plant. IAA influences cell elongation, not cell division or 'growth' in general.

OrganEffect of IAAResult
ShootHigher concentration on the shaded side increases the rate of cell elongationShaded side grows faster, so the shoot bends towards light (positive phototropism)
RootIAA inhibits cell elongationWhere IAA is more concentrated that side elongates more slowly than the other side; root bends away from light (negative phototropism) and, with gravity, downwards

Be careful not to mix up the effect of IAA on root cells and on shoot cells. In roots the lower side, with more IAA, elongates at a slower rate than the upper side.

Section 4 of 27Investigating the effect of IAA on root growth

Investigating the effect of IAA on root growth
Use a scalpel to cut a 1 cm section from the root tip of each seedling.
Mark the root tips at 2 mm intervals.
Treat groups of seedlings in different conditions, for example group A without IAA, group B with IAA, group C in directional light,
  • and measure the elongation after a set time.

Interpretationroots with added IAA do not grow as long as those in group A because IAA inhibits elongation.

Investigating the effect of IAA on root growth
IAA does not redistribute in response to light, so with uniform lighting there is an equal IAA concentration on both sides of the root tip.
In group C (directional light) we would expect the root to bend away from the light: there is a greater concentration of IAA on the shaded side,
  • so elongation is inhibited there while the illuminated side grows at a faster rate.
Phototropism is a directional response to light.

Section 5 of 27Worked examples

  1. State the name of the group of growth factors to which IAA belongs, and where it is made. [2]2 marks

  2. Explain how IAA causes a shoot to grow towards light. [3]3 marks

  3. Explain why a root placed in directional light bends away from the light. [3]3 marks

Section 6 of 27Taxes and kineses

Taxes and kineses are simple, innate responses that enable mobile organisms to remain in a favourable environment; they respond to stimuli such as light, humidity and chemicals. Kineses (singular kinesis) are non-directional responses involving random movement: for example in bright light flatworms move faster and change direction more often, increasing the chance of leaving the unfavourable conditions, which also helps them avoid predation. Taxis involves moving towards (positive) or away from (negative) a stimulus.

ResponseDirectionMovement
KinesisNon-directionalRandom, speed or turning rate changes with stimulus intensity
TaxisDirectionalTowards or away from the stimulus

Do not confuse these with plant responses: a beetle moving away from light is a taxis, not a tropism.

Section 7 of 27Reflex arcs

Simple reflexes allow rapid, automatic responses that protect an organism from harm, e.g. the pupil reflex prevents bright light from damaging the retina and the coughing reflex stops obstructions entering the airways. The pathway is: stimulus → receptor → sensory neurone → relay neurone → motor neurone → effector → response. Be ready to apply this to unfamiliar examples.

Section 8 of 27Required practical: animal movement in woodlice

Environmental factors that influence animal movement can be investigated using equipment such as a choice chamber (with different humidity or light zones) or a paper maze.

1
Moisture investigation: place woodlice in a chamber with a moist side and a dry side, count how many are on each side after a set time,
  • then repeat at least twice more.
2
A soft paintbrush can be used to gently move the woodlice back to the centre between repeats.
3
Calculate the mean number of woodlice on the moist and dry sides across all trials.
4
Return the woodlice to where they were collected from at the end.
5
A paper maze can be used to investigate turning behaviour under different conditions.

Section 9 of 27The Pacinian corpuscle

Receptors are cells that detect stimuli and are specific: each type detects a different type of stimulus.

Pacinian corpuscles
Skin contains touch receptors sensitive to pressure and temperature changes. Pacinian corpuscles are receptors found deep in the skin that respond to changes in pressure.
They are found at sensory neurone endings and are made of many layers of membrane separated by gel.
Do not confuse them with other receptors; they differ in structure and location.

A generator potential is a change in the potential difference across the membrane of a nerve cell in response to a stimulus. Its size depends on the size of the stimulus.

Section 10 of 27Worked examples

  1. Distinguish between a taxis and a kinesis. [2]2 marks

  2. Describe how you would investigate the effect of moisture on the movement of woodlice. [4]4 marks

  3. Explain why a reflex helps survival. [2]2 marks

Section 11 of 27Generator potential in a Pacinian corpuscle

When pressure is applied, the layers of membrane deform and the stretch-mediated sodium ion channels in the sensory neurone ending open.

Generator potential in a Pacinian corpuscle
Sodium ions diffuse in,
  • and the influx of sodium ions establishes an electrical potential difference across the membrane of the axon: this is the generator potential.
Stretch-mediated channels
Always describe the sodium ion channels that open in response to pressure as stretch-mediated channels.
A larger stimulus opens more channels, giving a larger generator potential; if it reaches the threshold an action potential is triggered.

Section 12 of 27Investigating touch and temperature receptors

It is possible to investigate receptors in human skin, for example the sensitivity of temperature receptors using warm and cool objects, and the effect of distance between two points of contact on the accuracy of touch perception, using a blunt pair of scissors or an unfolded paperclip.

1
Open the scissors or paperclip to a measured distance, e.g. 4 cm; use a ruler to confirm the spacing.
2
Seat the subject comfortably with their eyes covered.
3
Touch the skin with one or two points at random, and ask the subject whether they felt one or two.
4
Repeat at different distances and different skin areas.

Areas such as the fingertips and soles of the feet have much higher resolution: two points can be identified when very close together, because of a higher density of touch receptors. A limitation is that only one subject is used; ideally the experiment would be repeated with a number of subjects to achieve more reliable results.

Section 13 of 27Habituation of touch receptors

Over time touch receptors in the skin can become habituated to certain stimuli: the response to a stimulus decreases after repeated or prolonged exposure. This is why you stop noticing clothing on your skin.

Section 14 of 27The human retina: rods and cones

The eye is a sense organ containing receptors sensitive to light intensity and wavelength.

The human retina: rods and cones
Light receptors are located in the retina, a layer of cells at the back of the eye; the eye focuses light onto the retina (the details of eye structure need not be learned).
The retina contains rod cells, distributed across the entire retina, and cone cells, mostly concentrated at the fovea.
FeatureRodsCones
Sensitivity to lightHigh: many rods connect to a single bipolar neurone, so summation lets a small amount of light reach thresholdLower: each cone connects to its own bipolar neurone
WavelengthNot sensitive to different wavelengths: black and white visionThree types (red-, green-, blue-sensitive) allow colour vision
Visual acuityLowHigh

When red-, blue- and green-sensitive cones are stimulated in different numbers and locations, colour images are generated. A stimulated receptor generates an impulse in the bipolar cell.

Section 15 of 27Worked examples

  1. Explain how pressure on a Pacinian corpuscle leads to a generator potential. [4]4 marks

  2. Explain why rods are more sensitive to light than cones. [3]3 marks

  3. Suggest why the results of the two-point discrimination experiment are not reliable. [2]2 marks

Section 16 of 27Why cones give detailed images

Each cone cell connects to its own bipolar neurone and produces its own impulse when stimulated, so information from each cone is interpreted by the brain as a separate point and detailed images are generated. Because of the type of connection each cell type forms with its bipolar neurones, and the pigments they contain, rod cells are highly sensitive to light (many rods share one bipolar neurone, giving summation), can only generate black and white images and give low visual acuity; cone cells are less sensitive to light but give colour vision and high visual acuity.

Section 17 of 27Myogenic stimulation of the heart

Heart muscle contraction is myogenic: the heart beats at a baseline rate without any input from the nervous system.

Sinoatrial node (SAN)
The control involves the sinoatrial node (SAN), a group of cells in the wall of the right atrium, often called the pacemaker,
  • which initiates a wave of depolarisation across the atria; the wave then reaches the atrioventricular node (AVN),
    • and passes down the bundle of His and Purkyne fibres to the ventricles, spreading in a coordinated manner.
Do not confuse the two nodes: the SAN initiates the heartbeat; the AVN conducts electrical activity from the atria to the ventricles (after a short delay).

Section 18 of 27Changing heart rate

Although initiation is myogenic, heart rate has to change from baseline at times.

Changing heart rate
Autonomic nervous system
It is controlled by the cardioregulatory centre in the medulla of the brain, connected to the SAN by nerves of the autonomic nervous system,
  • which controls unconscious activities such as heart rate, breathing and digestion.
Sympathetic
It has two branches: the sympathetic branch (speeds up the heart) and the parasympathetic branch (slows it down).
The SAN can change its rate of initiation in response to this stimulation; do not confuse the roles of the SAN and the autonomic nervous system.

Which nerve the medulla uses depends on information from receptors in blood vessels: chemoreceptors detect blood pH/CO2 and pressure receptors detect blood pressure.

StimulusPathwayEffect
High blood CO2 (low pH) detected by chemoreceptorsImpulses to medulla, then sympathetic neurones to SAN; noradrenaline releasedIncreases heart rate
Low blood CO2 detected by chemoreceptors, or high blood pressure detected by pressure receptors1. Impulses along sensory neurones to the medulla; 2. impulses along parasympathetic neurones to the SAN; 3. acetylcholine released, binding to receptors on the SAN; 4. SAN decreases frequency of heartbeat initiationDecreases heart rate

Section 19 of 27Investigating factors affecting heart rate

The effect of a named variable on an organism's heart rate can be investigated, for example caffeine on human heart rate.

Investigating factors affecting heart rate
Volunteers should be resting and should not consume other food or drink.
Record the heart rate of each individual before the caffeine, give the caffeine,
  • then record heart rates again, repeating every 15 minutes for 2 hours.
This shows the duration for which caffeine has an effect on heart rate.

Section 20 of 27Worked examples

  1. Distinguish between the roles of the SAN and the AVN. [2]2 marks

  2. Explain how a rise in blood pressure leads to a slower heart rate. [4]4 marks

  3. Explain why the heart is described as myogenic. [1]1 mark

Section 21 of 27Why sample size matters

Investigations on living organisms, such as the effect of caffeine on heart rate in humans, are affected by natural variation between individuals.

Sample size
Using a larger sample size (more volunteers) reduces the effect of anomalous results and gives a mean that better represents the population.
Volunteers should be as similar as possible in factors such as age, fitness and caffeine habits, and all should be resting.

Section 22 of 27Repeats and reliability

A single measurement may be affected by error.

Taking repeat measurements of each volunteer's heart rate, at regular intervals (for example every 15 minutes for 2 hours in the caffeine practical), allows anomalies to be identified and a mean to be calculated.
Reliable
Results are reliable if repeats give similar values, and the more repeats and subjects used, the more confident you can be that a trend is real.

Section 23 of 27Controlling variables and analysing

1
Independent variable: the dose of caffeine (including a control with no caffeine or a placebo).
2
Dependent variable: heart rate (beats per minute).
3
Control variables: resting state, no other food or drink, time of day, and the same method of measuring pulse.
4
Analysis: calculate mean heart rate for each time and plot a graph; a statistical test can be used to decide whether differences are significant.

Section 24 of 27Evaluating the investigation

When evaluating, comment on sources of error and improvement: a small number of volunteers, volunteers who differ in habitual caffeine intake, different times since last meal, and counting pulse by hand (use a heart rate monitor for greater precision). Improvements include a larger sample, repeats of each measurement, a placebo control and matching volunteers.

Section 25 of 27Worked examples

  1. Explain why several volunteers should be used in an investigation of the effect of caffeine on heart rate. [2]2 marks

  2. Suggest why heart rate is measured again every 15 minutes for 2 hours. [2]2 marks

Section 26 of 27Key terms

TermMeaning
Growth factorA chemical, such as IAA, that affects growth in plants.
AuxinA group of plant growth factors, including IAA, that affect cell elongation.
IAA (indoleacetic acid)An auxin made in the tips of roots and shoots.
TropismA directional growth response of a plant to a directional stimulus.
PhototropismA directional growth response to light.
Cell elongationIncrease in cell length, producing growth.
TermMeaning
KinesisA non-directional response involving random movement.
TaxisA directional movement towards or away from a stimulus.
Reflex arcThe pathway from receptor to effector for an automatic response.
ReceptorA cell that detects a specific type of stimulus.
Pacinian corpuscleA pressure receptor deep in the skin made of layers of membrane separated by gel.
Generator potentialA change in the potential difference across a receptor membrane in response to a stimulus.
TermMeaning
Stretch-mediated channelA sodium ion channel that opens when the membrane is stretched or deformed.
HabituationA decreased response to a stimulus after repeated or prolonged exposure.
RetinaLayer of cells at the back of the eye containing light receptors.
Rod cellLight receptor with high sensitivity; many rods share one bipolar neurone.
Cone cellLight receptor sensitive to different wavelengths, allowing colour vision.
SummationAdding together of effects of several stimuli to reach threshold.
TermMeaning
MyogenicContraction that originates within the muscle itself, without nervous stimulation.
Sinoatrial node (SAN)Group of cells in the right atrium wall acting as the heart's pacemaker.
Atrioventricular node (AVN)Node that conducts electrical activity from atria to ventricles.
Cardioregulatory centreRegion of the medulla controlling heart rate through the autonomic nervous system.
Sympathetic nervous systemBranch of the autonomic nervous system that increases heart rate.
Parasympathetic nervous systemBranch that decreases heart rate.
AcetylcholineNeurotransmitter released by parasympathetic neurones onto the SAN.
TermMeaning
Sample sizeThe number of individuals or measurements in an investigation.
RepeatDoing the same measurement again to check consistency.
ReliabilityHow consistent results are when repeated.
Control variableA variable kept constant to make a test fair.

Section 27 of 27Exam technique

1
Calling IAA a plant hormone — use 'growth factor'.
2
Saying IAA stimulates cell division; it affects cell elongation.
3
Giving the same effect of IAA for roots and shoots; high concentrations stimulate elongation in shoots but inhibit it in roots.
4
Forgetting that the response is a directional growth response (tropism), not a movement of the whole organism.
1
Calling the movement of a beetle away from light a tropism — it is a taxis.
2
Describing kinesis as movement towards a stimulus.
3
Forgetting that Pacinian corpuscles detect pressure, not temperature.
4
Leaving out the relay neurone from the reflex arc.
1
Calling the sodium channels in a Pacinian corpuscle 'voltage-gated' — they are stretch-mediated.
2
Saying a bigger stimulus gives a bigger action potential — a bigger generator potential, but action potentials are all-or-nothing.
3
Forgetting that cones are responsible for colour and acuity, rods for sensitivity.
4
Describing habituation as damage to the receptor — the response decreases after repeated exposure.
1
Saying the nervous system starts the heartbeat — the SAN does, and nerves only modify the rate.
2
Mixing up the SAN and AVN.
3
Mixing up sympathetic (speeds up) and parasympathetic (slows down) pathways.
4
Omitting the medulla and the chemoreceptor or pressure receptor in the description of pathways.
1
Believing a single measurement is enough.
2
Confusing reliability (repeatability) with validity.
3
Not using a control group.
4
Forgetting to standardise conditions such as rest and other food and drink.

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