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Movement into and out of Cells

3. Movement into and out of Cells — Cambridge IGCSE Biology 0610 Revision Notes & Topical Questions

5 min read 11 parts Lesson 1 of 4 1 quiz

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Curriculum Equation Key قوانين الرقم الهيدروجيني والاتزان الأيوني - pH & Acid-Base Equilibrium FormulasFast Exam Solution
pH = -log[H⁺] | pOH = -log[OH⁻] | pH + pOH = 14 | [H⁺][OH⁻] = 10⁻¹⁴
Word Equation & Conditions: الرقم الهيدروجيني = -لوغاريتم تركيز أيون الهيدروجين | pH + pOH = 14
Location & Specifications: الاتزان الأيوني، كيمياء 3 ثانوي، التحصيلي، و AP Chemistry.
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Lesson summary

3. Movement into and out of Cells — IGCSE BIOLOGY notes: Explain diffusion, osmosis and active transport, and predict how cells respond to the solutions around them.Diffusion = the net movement of particles from a region of higher concentration to a region of lower concentration,…

  • Passive process
  • Partially permeable membrane
  • Concentration gradient
  • Temperature
  • Surface area to volume ratio
  • Water potential

Part 1 of 9Diffusion

1Diffusion
Diffusion the net movement of particles from a region of higher concentration to a region of lower concentration, down a concentration gradient,
  • as a result of their random movement.
2Passive process
  • Passive process it needs no energy from the cell;
    • the energy comes from the kinetic energy of random (Brownian) motion.
3Partially permeable membrane
  • Partially permeable membrane the cell membrane lets some molecules cross easily and others with difficulty or not at all.
  • The simplest selection is by molecule size.
4Uses
  • Uses organisms obtain requirements, remove wastes and carry out gas exchange for respiration by diffusion.

Part 2 of 9Examples of diffusion in organisms

SiteSubstanceFromTo
Small intestineGlucose, amino acids, fatty acids, glycerolLumen of the intestineBlood and lymph in the villi
LeafOxygenAir spaces between mesophyll cellsMitochondria in all cells
LeafCarbon dioxideAir spaces between mesophyll cellsChloroplasts in mesophyll cells
LeafWater vapourStomatal poresAir outside the stomata
LungsOxygenAlveolar air spaceBlood in capillaries around the alveoli
LungsCarbon dioxideBlood in capillaries around the alveoliAlveolar air space

Part 3 of 9Factors that affect the rate of diffusion

1Concentration gradient
  • Concentration gradient the greater the difference across the membrane, the faster the net diffusion.
  • More molecules on the high side move across than from the low side.
2Temperature
  • Temperature higher temperature gives molecules more kinetic energy, so net diffusion is faster.
3Distance
  • Distance the shorter the distance, the faster the diffusion.
  • Capillary and alveolar walls are one cell thick.
4Surface area to volume ratio
  • Surface area to volume ratio the larger a cell or structure, the smaller its ratio and the slower the exchange.
  • Root hair cells and the folded lining of the ileum have increased surface area.

Part 4 of 9Water as a solvent

Transport
  • Transport dissolved substances are carried in the xylem, the phloem and the blood.
Digestion
  • Digestion dissolved food molecules can be moved from the alimentary canal to cells all over the body.
Excretion
  • Excretion urea and excess salts dissolve in water and are removed in urine.
Cytoplasm
  • Cytoplasm water is a major part of cytoplasm and allows metabolic reactions to happen.

Part 5 of 9Osmosis

Osmosis across a partially permeable membrane
Osmosis across a partially permeable membrane
Osmosis
Osmosis the net movement of water molecules from a region of higher water potential (dilute solution) to a region of lower water potential (concentrated solution) through a partially permeable membrane.
Water potential
  • Water potential high water potential means a dilute solution with low solute concentration;
    • low water potential means a concentrated solution.
Membrane
  • Membrane the cell membrane lets small molecules such as water through but not larger solute molecules.

Part 6 of 9Osmosis in plant and animal cells

Solution outsidePlant cellAnimal cell
Dilute (higher water potential)Water enters; vacuole expands and pushes the membrane against the wall; cell becomes turgid and does not burstWater enters; with no wall, the membrane stretches and the cell bursts
Same concentrationNo net movement; cell is unchangedNo net movement; cell is unchanged
Concentrated (lower water potential)Water leaves; cell becomes flaccid and may be plasmolysed (membrane pulls away from wall)Water leaves; cell shrivels (crenation)

Part 7 of 9Osmosis experiments

1
Cut cylinders of potato (or radish) of identical size and measure the initial mass of each.
2
Place one cylinder in each of distilled water and sucrose solutions of increasing concentration.
3
Leave for the same time (20–30 minutes) at the same temperature.
4
Remove, blot dry to remove surface liquid and reweigh each cylinder.
5
Calculate the percentage change in mass for each solution.

Part 8 of 9Active transport

Active transport using ATP
Active transport using ATP
Active transport
Active transport
Active transport the movement of particles through a cell membrane from a region of lower concentration to a region of higher concentration, using energy from respiration.
Energy
  • Energy it is needed because particles move against the concentration gradient, opposite to diffusion.
Examples
  • Examples uptake of glucose by epithelial cells in the villi and by kidney tubules in the nephron.
  • Uptake of mineral ions from soil water by root hair cells.
Cell features
  • Cell features cells that actively transport have many mitochondria, and the process slows if respiration or oxygen supply falls.

Part 9 of 9Comparing diffusion, osmosis and active transport

FeatureDiffusionOsmosisActive transport
Particles movedAny, mainly gases and solutesWater onlyIons and molecules
DirectionDown a concentration gradientFrom high to low water potentialAgainst a concentration gradient
Energy from respirationNoNoYes
Membrane / proteinsNot requiredPartially permeable membraneCarrier proteins in the membrane

Key terms

TermMeaning
DiffusionNet movement of particles down a concentration gradient.
OsmosisNet movement of water through a partially permeable membrane from higher to lower water potential.
Active transportMovement of particles against a concentration gradient using energy from respiration.
TurgidA plant cell full of water, pushing on its cell wall.
FlaccidA plant cell that has lost water and is limp.
PlasmolysisThe cell membrane pulling away from the cell wall when water is lost.
CrenationShrivelling of an animal cell that has lost water by osmosis.
Partially permeable membraneA membrane that lets some molecules through and not others.

Avoid these mistakes

1
Saying osmosis moves solute — it is the movement of water only.
2
Using concentration of water loosely — use higher or lower water potential, or dilute versus concentrated solution.
3
Thinking active transport works without oxygen — it needs energy from respiration.
4
Believing plant cells burst in dilute solution — the cell wall prevents bursting and the cell becomes turgid.

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Cambridge 9700 AS Biology Core Formula

Magnification Triangle & Unit Conversion

Formula: M = I / A  ⇔  A = I / M  ⇔  I = M × A

Units: 1 mm = 1,000 μm = 1,000,000 nm.
Rule: Convert Image size (ruler mm) to μm by ×1,000 before dividing by Actual size.

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