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B1.6B1. Characteristics of Living Organisms and Cells

Active transport — Combined Science 0653 - Biology Revision Notes & Topical Questions

5 min read 8 parts Lesson 8 of 14 Unit quiz

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B1.6 Active transport (Part 2) — COMBINED SCIENCE notes: Compare active transport with diffusion and osmosis and describe what affects its rate.FeatureDiffusionOsmosisActive transportParticlesAny small moleculesWater onlyIons, glucose and amino acidsDirectionHigh to low concentra…

  • Mitochondria
  • Respiration inhibited
  • Temperature
  • Oxygen supply
  • Carrier proteins
  • Root hair cells

Section 1 of 8Comparison of the three processes

FeatureDiffusionOsmosisActive transport
ParticlesAny small moleculesWater onlyIons, glucose and amino acids
DirectionHigh to low concentrationHigh to low water potentialLow to high concentration
Energy from respirationNot neededNot neededNeeded
MembraneNot essentialPartially permeableCarrier proteins

Section 2 of 8Evidence that active transport uses energy

Mitochondria
  • Mitochondria cells that carry out a lot of active transport, such as root hair cells and cells lining the small intestine, have many mitochondria.
Respiration inhibited
  • Respiration inhibited active transport slows or stops when respiration is stopped.
  • A lack of oxygen or a poison such as cyanide has this effect.

Section 3 of 8Factors that affect the rate

Temperature
  • Temperature the rate rises with temperature, up to the point where proteins denature.
  • A higher temperature raises the rate of respiration, which supplies energy.
Oxygen supply
  • Oxygen supply more oxygen allows faster aerobic respiration, so more energy is available.
Carrier proteins
  • Carrier proteins the number in the membrane limits the rate.
  • Cells specialised for absorption have many carriers.

Section 4 of 8Examples in context

Root hair cells
  • Root hair cells take up mineral ions such as nitrate from dilute soil water.
Small intestine
  • Small intestine absorbs glucose when its concentration in the gut is lower than in the blood.
Kidney tubules
  • Kidney tubules reabsorb glucose back into the blood.

Section 5 of 8Choosing the process

1Down a gradient
  • Down a gradient use diffusion or osmosis, with no energy.
2Against a gradient
  • Against a gradient use active transport, with energy from respiration.

Section 6 of 8More from the specification

1Active transport definition
  • Active transport is the movement of dissolved substances or ions across a cell membrane from a region of lower concentration to a region of higher concentration, requiring cellular energy from ATP.
    • (against a concentration gradient)
2Active transport in plants
  • Root hair cells in plants use specialized carrier proteins to absorb mineral ions from dilute soil water solution into plant root tissue against a steep concentration gradient.
    • (such as nitrates and magnesium)
3Diffusion through Visking tubing
  • In model laboratory experiments, dialysis (Visking) tubing demonstrates passive diffusion and selective permeability,
  • where smaller solute molecules diffuse across pores into external water solution while large macromolecules are retained.
4Importance in living organisms
  • Living organisms depend on active transport for vital physiological functions,
  • including glucose reabsorption in the mammalian nephron and nutrient absorption across intestinal epithelial tissue.
5Solvent
  • Liquid in which a solute dissolves.
6Solute
  • Substance that dissolves in a solvent.
7Carbon dioxide
  • Gas that turns limewater cloudy.
8Dialysis tubing
  • A non-living partially permeable membrane.
9Tissue
  • A group of similar cells working together to do one job.
10Organism
  • A whole living thing made of organ systems.

Section 7 of 8Key terms

TermMeaning
Active transportMovement against a concentration gradient using energy.
RespirationThe release of energy from food in cells.
Concentration gradientA difference in concentration between two regions.
MitochondrionThe organelle that releases energy in respiration.
Carrier proteinA membrane protein that carries particles across.
PassiveNot needing energy from respiration.

Section 8 of 8Exam technique

1
Saying osmosis needs energy — osmosis is passive.
2
Saying solutes move in osmosis — only water moves.
3
Writing that active transport is from high to low concentration — it is low to high.
4
Forgetting carrier proteins — active transport needs them.

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