4.6.1Additional lessons
Osmosis — IB Biology HL Revision Notes & Topical Questions
5 min read 5 parts Lesson 51 of 101
Part 1 of 5Water as a Solvent

Polarity
- Oxygen attracts the shared electrons more strongly than hydrogen, so water has a slightly negative oxygen end and positive hydrogen ends.
Hydration shells
- Water molecules cluster around ions and polar molecules, separating them and keeping them dissolved in solution.
Hydrogen bonds
- Water also forms hydrogen bonds with polar solutes such as glucose, helping them to dissolve.
Non-polar substances
- Lipids are hydrophobic and do not dissolve, which allows membranes to separate watery compartments.
Part 2 of 5Defining Osmosis
Water diffuses in both directions, but more water moves towards the more concentrated solution.
Osmosis
- Net movement of water molecules across a partially permeable membrane from a lower solute concentration to a higher solute concentration.
Passive
- The process needs no ATP, because water moves down its concentration gradient.
Membrane
- The membrane lets water pass, but restricts larger solutes, so water movement balances the concentrations.
Aquaporins
- Channel proteins speed up the movement of water across membranes without changing the direction.
Part 3 of 5Comparing Solutions
Hypotonic
- A solution with a lower solute concentration than the cell, so water enters the cell by osmosis.
Hypertonic
- A solution with a higher solute concentration than the cell, so water leaves the cell.
Isotonic
- A solution with the same solute concentration as the cell, so there is no net movement of water.
Relative terms
- Tonicity always compares two solutions, so a solution is hypertonic or hypotonic only relative to another.
| Outside solution | Net water movement | Cell result |
|---|---|---|
| Hypotonic | Into the cell | Swells |
| Isotonic | None | No change |
| Hypertonic | Out of the cell | Shrinks |
Part 4 of 5Predicting Direction
1
Identify the two solutions on either side of the partially permeable membrane.
2
Decide which solution has the higher total solute concentration.
3
Water moves from the more dilute solution to the more concentrated solution.
4
State the outcome: the volume of the concentrated side rises while the dilute side falls.
Part 5 of 5Exam Checklist & Tips
4.6.1 – Key Points for the Exam
- Osmosis The net movement of water across a partially permeable membrane towards the higher solute concentration.
- Hypotonic Having a lower solute concentration than another solution.
- Hypertonic Having a higher solute concentration than another solution.
- Isotonic Having equal solute concentrations.
Common Mistakes
- Saying that solutes move in osmosis; it is the movement of water.
- Mixing up hypotonic and hypertonic.
- Stating that osmosis stops at equilibrium; water still moves, but with no net change.
- Describing water moving from high solute to low solute concentration.
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