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Course: AQA A Level Biology 7402
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AQA A Level Biology 7402

1. Biological Molecules

0/102

2. Cell Structure

0/95

3. Exchange and Transport

0/98

4. Genetics, Variation and Interdependence

0/94

5. Energy Transfers In and Between Organisms (A Level only)

0/63

6. Organisms Respond to Changes in their Environments (A Level only)

0/93

7. Genetics, Populations, Evolution and Ecosystems (A Level only)

0/55

8. The Control of Gene Expression (A Level only)

0/72

9. Exam Guidance and Skills

0/38
Quiz

8.3.4 Sequencing Methods – Exam questions

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  • Questions count: 12
  • Passing grade: B+
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1.
In Sanger sequencing, what stops chain elongation?
Restriction enzymes
Primers
Incorporation of a dideoxynucleotide
High temperature
2.
Which statement best describes DNA sequencing?
Determining the order of nucleotide bases in a DNA molecule.
The use of computers to store, compare and analyse large biological data sets such as genome sequences.
Comparing the pattern of variable DNA regions (e.g. short tandem repeats) between individuals.
A technique that separates DNA fragments by size as they move through a gel in an electric field.
3.
According to the comparison table in this lesson, what is the Key steps of Sequencing?
According to the comparison table in this lesson, what is the Key steps of Sequencing?
Biotechnology techniques
95 °C denature, 55 °C anneal, 72 °C extend
Load, run current, stain
Dideoxy / next-gen
Guide RNA targets DNA
4.
Cost of sequencing a human genome over time is shown. By roughly what factor has cost fallen from 2003 to 2023?
About ten million times
About ten times
About a thousand times
It has risen
5.
The graph (log scale) shows sequencing cost. What is the main consequence of the fall?
Dr Dabour Hub Academy - figure 1
Sequencing is now impossible
Genome sequencing is now affordable for widespread medical use
Fewer genomes are sequenced
Costs rose sharply
6.
Why is Taq polymerase used in PCR?
It cuts DNA at specific sites
It is not denatured at high temperatures
It joins DNA fragments
It works best at 37 °C
7.
During gel electrophoresis DNA moves towards the:
Negative electrode
Top of the gel
Positive electrode
Well
8.
What happens in the annealing step of PCR?
Strands separate
Primers bind to complementary sequences
Nucleotides are added
DNA is cut
9.
Which fragments travel furthest in a gel?
The smallest
The largest
Those with most G-C pairs
All travel equally
10.
Why do STRs make useful markers in DNA profiling?
They code for proteins
They are identical in all people
They are only found in mitochondria
The number of repeats varies greatly between individuals
11.
Which statement best describes Short tandem repeat (STR)?
A short DNA sequence repeated many times in a row; the number of repeats varies between people.
A short single-stranded DNA sequence complementary to the start of the target region, providing a start point for DNA polymerase.
Comparing the pattern of variable DNA regions (e.g. short tandem repeats) between individuals.
A technique that makes many copies of a specific DNA sequence by repeated cycles of heating and cooling.
12.
Which statement best describes Dideoxynucleotide?
A short single-stranded DNA sequence complementary to the start of the target region, providing a start point for DNA polymerase.
A technique that makes many copies of a specific DNA sequence by repeated cycles of heating and cooling.
A heat-stable DNA polymerase from the bacterium Thermus aquaticus, used in PCR.
A modified nucleotide lacking the 3' OH group, which terminates DNA synthesis when added.
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Part of 8. The Control of Gene Expression (A Level only) — see every lesson and quiz in this unit.
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