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Course: AQA A Level Biology 7402
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AQA A Level Biology 7402

1. Biological Molecules

0/102

2. Cell Structure

0/95

3. Exchange and Transport

0/98

4. Genetics, Variation and Interdependence

0/94

5. Energy Transfers In and Between Organisms (A Level only)

0/63

6. Organisms Respond to Changes in their Environments (A Level only)

0/93

7. Genetics, Populations, Evolution and Ecosystems (A Level only)

0/55

8. The Control of Gene Expression (A Level only)

0/72

9. Exam Guidance and Skills

0/38
Quiz

8.4.3 Investigating the Specificity of Restriction Enzymes – Exam questions

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  • Questions count: 9
  • Passing grade: B+
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1.

The specificity of an enzyme is due to

its size
its solubility
the shape of its active site, determined by its tertiary structure
its primary structure only
2.
A mutation changed a polar amino acid into a nonpolar one far from the active site. How might this alter the enzyme\'s substrate specificity?
A mutation changed a polar amino acid into a nonpolar one far from the active site. How might this alter the enzyme's substrate specificity?
By changing the enzyme pH optimum
By changing the enzyme location in the cell
By changing the shape of the protein
An amino acid change away from the active site cannot alter substrate specificity.
3.
Restriction enzymes
join DNA fragments
make proteins
cut DNA at specific recognition sequences
copy RNA into DNA
4.
The table shows the restriction enzyme recognition sites present on a circular piece of DNA 4.0 kilobases (1 kb = 1,000 bases) long.
SAT Biology - Question 281
A
B
C
D
E
5.
Which statement best describes Restriction enzyme?
Which statement best describes Restriction enzyme?
Cuts DNA at specific sequences.
Bacteria take up foreign DNA.
Organism with altered genes.
Separates DNA by size; small moves farther.
6.
A restriction enzyme cuts at GAATTC. How many times will it cut the sequence in the table?
Once
Three times
Twice
Not at all
7.
The diagram shows DNA cut by a restriction enzyme. Why must the gene and plasmid be cut with the same enzyme?
Dr Dabour Hub Academy - figure 1
So the DNA becomes blunt
So ligase is not needed
So PCR can start
So their single-stranded sticky ends are complementary and pair up
8.
Restriction enzymes cut DNA and DNA ligase joins it. True or false?
True
False
False, the reverse
True only in PCR
9.
Restriction enzymes evolved in bacteria as a defence against viruses. True or false?
False
False, they were made in labs
True only in plants
True
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Part of 8. The Control of Gene Expression (A Level only) — see every lesson and quiz in this unit.
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