3.3.6Additional lessons
Carbon Dioxide Enrichment Experiments — IB Biology SL Revision Notes & Topical Questions
5 min read 6 parts Lesson 61 of 142
Part 1 of 6Purpose and Design

Aim
- Enrichment experiments test how plant growth responds to higher carbon dioxide concentrations.
Greenhouses
- Growers can raise carbon dioxide in enclosed greenhouses, which increases photosynthesis and crop yield.
Field experiments
- Free-air carbon dioxide enrichment rigs release the gas around plants in open fields without enclosing them.
Controls
- Control plots are grown at normal atmospheric concentration under the same conditions.
Measurements
- Growth is measured as height, leaf area, biomass or yield, using dry mass for accuracy.
Part 2 of 6Findings
1
Many plants show faster growth at higher carbon dioxide, because carbon dioxide can limit photosynthesis.
2
The effect is often smaller than predicted, because other factors such as water and minerals then limit the rate.
3
Over a longer time plants may acclimatise, and the extra growth may decrease.
4
Stomata of some plants partly close at high carbon dioxide, which may also reduce water loss.
5
Results are used to predict how crops and ecosystems may respond to rising atmospheric carbon dioxide.
Part 3 of 6Evaluating the Evidence
Evaluating the Evidence
Replication
- Several plots and repeated measurements show whether differences are likely to be real.
Limitations
- An enclosed experiment may differ from natural conditions, and a short study may not show long-term effects.
Application
- Higher yield may be offset by lower nutritional quality or by climate change effects such as drought.
Statistics
- A statistical test can show whether differences in mean biomass are significant.
Part 4 of 6Link to Climate
Link to Climate
Atmosphere
- Atmospheric carbon dioxide concentration has risen since the industrial revolution, mainly from burning fossil fuels.
Carbon sink
- Plants take up carbon dioxide in photosynthesis, so growth responses affect how much the atmosphere retains.
Prediction
- Results from enrichment experiments help to model food production under future climates.
Part 5 of 6Worked Example
Question
- In a field trial the control plot yields 6.0 tonnes per hectare and the enriched plot yields 6.9.
- Calculate the percentage increase.
- Increase = 6.9 − 6.0 = 0.9 tonnes per hectare.
- Percentage = 0.9 ÷ 6.0 × 100 = 15 %.
Answer A 15 % increase in yield (example data).
Part 6 of 6Exam Checklist & Tips
3.3.6 – Key Points for the Exam
- Carbon dioxide enrichment Raising carbon dioxide concentration around plants to study or increase growth.
- Biomass The dry mass of organic matter in an organism or area.
- Acclimatisation Adjustment of an organism to a change in its conditions.
- Control plot An area grown under normal conditions for comparison.
Common Mistakes
- Assuming that more carbon dioxide always means proportionally more growth.
- Measuring fresh mass, which changes with water content, instead of dry mass.
- Forgetting that the control and treatment must have the same temperature, light and water.
- Stating that the result applies to all plants without evidence.
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