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4.5.2Additional lessons

Osmosis in Cells — IB Biology SL Revision Notes & Topical Questions

5 min read 5 parts Lesson 114 of 142

📚 IB Biology SL Hub / Topical Revision
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Curriculum Equation Key قوانين الرقم الهيدروجيني والاتزان الأيوني - pH & Acid-Base Equilibrium FormulasFast Exam Solution
pH = -log[H⁺] | pOH = -log[OH⁻] | pH + pOH = 14 | [H⁺][OH⁻] = 10⁻¹⁴
Word Equation & Conditions: الرقم الهيدروجيني = -لوغاريتم تركيز أيون الهيدروجين | pH + pOH = 14
Location & Specifications: الاتزان الأيوني، كيمياء 3 ثانوي، التحصيلي، و AP Chemistry.
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Part 1 of 5Animal Cells

Red blood cells show the effects clearly because they are surrounded by plasma whose concentration must stay steady.

No cell wall
  • No cell wall Animal cells have only a flexible cell surface membrane, so they cannot resist large changes in volume caused by water movement.
In hypotonic fluid
  • In hypotonic fluid Water enters by net osmosis, the cell swells, and the membrane can stretch too far so that the cell bursts, which is called lysis.
In hypertonic fluid
  • In hypertonic fluid Water leaves by net osmosis, the cell shrinks and its surface becomes shrivelled and spiky, which is called crenation.
In isotonic fluid
  • In isotonic fluid Water movement in each direction is balanced, so the cell keeps its normal volume and shape in dynamic equilibrium.

Part 2 of 5Plant Cells

Plant Cells
Hypotonic outside
  • Hypotonic outside Water enters the vacuole, the protoplast expands and presses on the cell wall, building up turgor pressure.
Turgid cell
  • Turgid cell A fully inflated, firm cell;
    • the inelastic cellulose wall resists further swelling so the cell does not burst.
Hypertonic outside
  • Hypertonic outside Water leaves the vacuole, the protoplast shrinks and eventually pulls away from the wall, which is plasmolysis.
Flaccid and wilting
  • Flaccid and wilting When cells lose turgor the plant loses support, and leaves and stems droop;
    • watering restores turgor.
Role of turgor
  • Role of turgor Turgid cells hold leaves out to the light and keep stems upright, so turgor works as support in non-woody tissue.
Cell typeHypotonic solutionHypertonic solution
AnimalSwells and may burst (lysis)Shrinks, crenated
PlantBecomes turgid, wall prevents burstingProtoplast shrinks, plasmolysis

Part 3 of 5Living Things in Dilute Water

1
Freshwater protozoa such as Amoeba live in water that is hypotonic to their cytoplasm, so water constantly enters by osmosis.
2
Without a control mechanism this inflow would raise internal pressure until the cell membrane ruptured.
3
Excess water collects in a contractile vacuole inside the cytoplasm.
4
The vacuole moves to the cell surface and expels its contents, using energy from ATP.
5
This cycle repeats continuously, keeping the volume and solute concentration of the cell steady.

Part 4 of 5Medical Applications

Intravenous drips
  • Intravenous drips Fluids given into the blood must be isotonic with plasma;
    • 0.9% sterile saline is commonly used for this purpose.
Hypotonic drip risk
  • Hypotonic drip risk Red blood cells would take in water and burst, reducing the oxygen-carrying capacity of the blood.
Hypertonic drip risk
  • Hypertonic drip risk Red blood cells would shrink and become crenated, and they may then block capillaries.
Organs for transplant
  • Organs for transplant Donated organs are bathed in an isotonic solution so that their cells neither swell nor shrink while stored.

Part 5 of 5Exam Checklist & Tips

4.5.2 – Key Points for the Exam

  • Lysis Bursting of an animal cell after excess water uptake.
  • Crenation Shrinking and shrivelling of an animal cell after water loss.
  • Turgor pressure Pressure of the protoplast pushing against the cell wall of a plant cell.
  • Plasmolysis Shrinking of the protoplast away from the cell wall when a plant cell loses water.
  • Contractile vacuole Organelle that collects and expels excess water in some freshwater unicellular organisms.

Common Mistakes

  • Saying plant cells burst in pure water; the inelastic wall resists and the cell becomes turgid instead.
  • Confusing the cell wall with the membrane; the wall is freely permeable, the membrane is partially permeable.
  • Writing that a plasmolysed cell has an empty gap that is air;
    • the space between wall and membrane is filled with external solution.
  • Calling the 0.9% saline drip hypotonic; it is chosen because it matches the concentration of blood plasma.

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Cambridge 9700 AS Biology Core Formula

Magnification Triangle & Unit Conversion

Formula: M = I / A  ⇔  A = I / M  ⇔  I = M × A

Units: 1 mm = 1,000 μm = 1,000,000 nm.
Rule: Convert Image size (ruler mm) to μm by ×1,000 before dividing by Actual size.

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