1.4.5Inquiry Process
Prokaryotic Cell Structure — IB Biology HL Revision Notes & Topical Questions
5 min read 5 parts Lesson 6 of 160
Curriculum Equation Key قوانين الرقم الهيدروجيني والاتزان الأيوني - pH & Acid-Base Equilibrium FormulasFast Exam Solution
pH = -log[H⁺] | pOH = -log[OH⁻] | pH + pOH = 14 | [H⁺][OH⁻] = 10⁻¹⁴
Word Equation & Conditions: الرقم الهيدروجيني = -لوغاريتم تركيز أيون الهيدروجين | pH + pOH = 14
Location & Specifications: الاتزان الأيوني، كيمياء 3 ثانوي، التحصيلي، و AP Chemistry.
Part 1 of 5Core Components
Prokaryotes are small, simple cells with no nucleus and no membrane-bound organelles.
Cell wall
- Cell wall A rigid layer of peptidoglycan outside the membrane that prevents bursting in dilute surroundings and keeps the cell shape.
Plasma membrane
- Plasma membrane A phospholipid bilayer controlling which substances enter and leave, and the site of many reactions such as respiration.
Cytoplasm
- Cytoplasm A watery solution of enzymes, salts and ribosomes in which the metabolic reactions of the cell take place.
70S ribosomes
- 70S ribosomes Small ribosomes lying free in the cytoplasm that translate messenger RNA into polypeptides for the cell.
Nucleoid
- Nucleoid A region holding one circular, naked DNA molecule that is not surrounded by a nuclear envelope.
Plasmid
- Plasmid A small circular DNA ring carrying extra genes, such as antibiotic resistance, that can pass between bacteria.
Part 2 of 5Surface Structures
Surface Structures
Flagellum
- Flagellum A long rotating protein filament that propels the bacterium through liquid towards favourable conditions.
Pili
- Pili Short hair-like protein projections used to attach to surfaces, and in conjugation to link two bacterial cells.
Capsule
- Capsule A sticky outer layer of polysaccharide in some species that protects against drying and engulfment by white blood cells.
Mesosome idea
- Mesosome idea Folds once drawn in the membrane are now regarded as artefacts of specimen preparation, not real structures.
Part 3 of 5Reading a Prokaryote in a Micrograph
1
Look first at the overall size; most bacteria measure about 1 to 5 micrometres, far smaller than a typical eukaryotic cell.
2
Search for a nucleus; its absence together with a pale nucleoid region of DNA marks the cell as prokaryotic.
3
Check for a clear cell wall outside the membrane, which is nearly always present in bacteria.
4
Note any flagella, pili or capsule, since these help decide the species and its method of movement or attachment.
5
Finish by labelling the structures and writing the function of each beside its label.
| Feature | Prokaryote |
|---|---|
| DNA | Naked, circular, in nucleoid |
| Ribosomes | 70S |
| Wall | Peptidoglycan |
| Organelles | None membrane-bound |
Part 4 of 5Worked Example
Question
- An electron micrograph shows a rod-shaped cell with no nucleus, a thick outer layer, a ring of DNA in a pale area and many small dark granules.
- Identify the cell type and justify the identification.
- The absence of a nucleus and of any membrane-bound organelles points to a prokaryote.
- The pale area holding a ring of DNA is the nucleoid, which has no nuclear envelope.
- The thick outer layer outside the plasma membrane is the cell wall, made of peptidoglycan.
- The small dark granules are 70S ribosomes, which are smaller than eukaryotic ribosomes.
Answer The cell is a bacterium (prokaryote): no nucleus, naked circular DNA in a nucleoid, peptidoglycan wall and 70S ribosomes.
Part 5 of 5Exam Checklist & Tips
1.4.5 – Key Points for the Exam
- Prokaryote A cell without a nucleus or membrane-bound organelles.
- Nucleoid The region of a prokaryotic cell containing its naked DNA.
- Peptidoglycan The sugar-amino acid polymer forming bacterial cell walls.
- Plasmid A small circular extra-chromosomal DNA molecule in bacteria.
- 70S ribosome The smaller ribosome type found in prokaryotes.
Common Mistakes
- Stating that prokaryotes have no DNA; they do, but it is not enclosed by a nuclear envelope.
- Saying the bacterial wall is made of cellulose; it is peptidoglycan.
- Calling the nucleoid a nucleus.
- Forgetting that prokaryotes still carry ribosomes, which are 70S not 80S.
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Cambridge 9700 AS Biology
Core Formula
Magnification Triangle & Unit Conversion
Formula: M = I / A ⇔ A = I / M ⇔ I = M × A
Units: 1 mm = 1,000 μm = 1,000,000 nm.
Rule: Convert Image size (ruler mm) to μm by ×1,000 before dividing by Actual size.