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2.7.2Inquiry Process

Identify Blood Vessels- Skills — IB Biology HL Revision Notes & Topical Questions

5 min read 5 parts Lesson 74 of 160

📚 IB Biology HL Hub / Topical Revision
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pH = -log[H⁺] | pOH = -log[OH⁻] | pH + pOH = 14 | [H⁺][OH⁻] = 10⁻¹⁴
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Section 1 of 5Clues in a Transverse Section

Relative thickness is a more reliable clue than colour or absolute size.

Artery
  • Artery Appears round with a thick wall, a small lumen and often a wavy inner lining because the elastic tissue has recoiled.
Vein
  • Vein Appears thinner walled and often irregular or collapsed, with a larger lumen compared with the artery next to it.
Capillary
  • Capillary Appears as a tiny tube with a wall just one cell thick and red blood cells passing through in single file.
Companions
  • Companions In tissue sections an artery and its accompanying vein often lie side by side, which makes comparison easy.

Section 2 of 5Step-by-Step Identification

1
Look at the overall shape: is the section round and regular or flattened and irregular?
2
Compare the thickness of the wall with the width of the lumen.
3
Look for layers of muscle and elastic tissue, which are thick in arteries and thinner in veins.
4
Check the lumen width: a large open lumen suggests a vein, while a very small tube with one-cell wall suggests a capillary.
5
State your conclusion with two or more features as evidence, linking them to function.

Section 3 of 5Measuring and Calculating

Measuring and Calculating
Magnification
  • Magnification Calculate magnification by dividing the measured image length by the real length, or use the scale bar if one is given.
Actual size
  • Actual size Actual size equals measured size divided by magnification, so a 24 mm image at ×60 represents 0.4 mm.
Wall to lumen ratio
  • Wall to lumen ratio Dividing wall thickness by lumen diameter gives a number that is larger in arteries than in veins.
Units
  • Units Convert measurements to the same unit, such as micrometres, before dividing.
VesselWall thickness (mm)Lumen diameter (mm)Ratio
Artery1.23.00.40
Vein0.34.50.07

Section 4 of 5Worked Example

Question

  • In a micrograph the wall of an artery measures 6 mm and its lumen measures 15 mm.
  • The magnification is ×5.
  • Calculate the actual wall thickness and the wall to lumen ratio.

  1. Actual wall thickness = 6 ÷ 5 = 1.2 mm.
  2. Actual lumen diameter = 15 ÷ 5 = 3.0 mm.
  3. Ratio = 1.2 ÷ 3.0 = 0.40.

Answer The wall is 1.2 mm thick and the wall to lumen ratio is 0.40.

Section 5 of 5Exam Checklist & Tips

2.7.2 – Key Points for the Exam

  • Transverse section A cut at right angles to the long axis of a structure.
  • Wall to lumen ratio Wall thickness divided by lumen diameter, used to compare vessels.
  • Scale bar A line on an image that represents a known real length.
  • Micrograph An image taken through a microscope.

Common Mistakes

  • Using colour alone to decide between artery and vein;
    • stained sections do not follow the red and blue convention.
  • Measuring wall thickness at an angle rather than across the wall.
  • Forgetting to convert units before calculating magnification.
  • Giving only one feature as evidence rather than relating wall, lumen and function.

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