2.8.3Inquiry Process
Skeletal Muscle — IB Biology HL Revision Notes & Topical Questions
5 min read 5 parts Lesson 88 of 160
Part 1 of 5Muscle Fibre Structure

Skeletal muscle is attached to bones and under voluntary control.
Multinucleate fibres
- Each fibre is a long cell formed by fusion of many cells, so it contains many nuclei just beneath the cell surface.
Myofibrils
- Cylindrical bundles of actin and myosin fill the fibre and give it a striped, or striated, appearance.
Sarcoplasmic reticulum
- This specialised endoplasmic reticulum surrounds the myofibrils and stores calcium ions for release when stimulated.
Mitochondria
- Many mitochondria between myofibrils generate the ATP needed for contraction and relaxation.
T-tubules
- Inward folds of the cell membrane carry the action potential rapidly into the middle of the fibre, so all myofibrils contract together.
Part 2 of 5Comparing Muscle Types
Comparing Muscle Types
Skeletal muscle
- Striated, voluntary and multinucleate; contracts quickly but tires.
Cardiac muscle
- Striated, involuntary and branched, with intercalated discs linking cells;
- it contracts rhythmically without tiring.
Smooth muscle
- Non-striated and involuntary, found in walls of gut and blood vessels; contracts slowly and can be sustained.
Fast and slow fibres
- Fast fibres contract quickly and rely on anaerobic respiration,
- while slow fibres have more myoglobin and mitochondria and resist fatigue.
| Property | Skeletal | Cardiac | Smooth |
|---|---|---|---|
| Striations | Yes | Yes | No |
| Control | Voluntary | Involuntary | Involuntary |
| Nuclei per cell | Many | One or two | One |
Part 3 of 5Muscle Control
1
A motor neuron releases acetylcholine at the neuromuscular junction.
2
The acetylcholine triggers an action potential in the muscle membrane, which spreads along T-tubules.
3
Calcium is released from the sarcoplasmic reticulum, causing the myofibrils to contract.
4
All the fibres supplied by one motor neuron, a motor unit, contract together.
5
Stronger contractions recruit more motor units, while a continuous stream of impulses maintains tension.
Part 4 of 5Worked Example
Question
- In relaxed muscle a sarcomere is 2.5 µm long.
- When contracted it measures 1.8 µm.
- Calculate the percentage decrease in length.
- Decrease = 2.5 − 1.8 = 0.7 µm.
- Percentage = 0.7 ÷ 2.5 × 100.
- = 28%.
Answer The sarcomere shortens by 28%.
Part 5 of 5Exam Checklist & Tips
2.8.3 – Key Points for the Exam
- Muscle fibre A single long, multinucleate muscle cell.
- Myofibril A cylindrical bundle of contractile filaments inside a muscle fibre.
- Motor unit One motor neuron and all the muscle fibres it stimulates.
- T-tubule A fold of the plasma membrane that carries action potentials deep into the fibre.
Common Mistakes
- Calling skeletal muscle fibres single-nucleus cells; they are multinucleate.
- Using 'striated' for smooth muscle.
- Confusing myofibrils with muscle fibres; fibres contain many myofibrils.
- Stating that the sarcoplasmic reticulum makes ATP; it stores calcium.
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