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2.7.5Inquiry Process

The Transpiration Stream — IB Biology HL Revision Notes & Topical Questions

5 min read 5 parts Lesson 77 of 160

📚 IB Biology HL Hub / Topical Revision
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Curriculum Equation Key قوانين الرقم الهيدروجيني والاتزان الأيوني - pH & Acid-Base Equilibrium FormulasFast Exam Solution
pH = -log[H⁺] | pOH = -log[OH⁻] | pH + pOH = 14 | [H⁺][OH⁻] = 10⁻¹⁴
Word Equation & Conditions: الرقم الهيدروجيني = -لوغاريتم تركيز أيون الهيدروجين | pH + pOH = 14
Location & Specifications: الاتزان الأيوني، كيمياء 3 ثانوي، التحصيلي، و AP Chemistry.
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Part 1 of 5The Cohesion-Tension Mechanism

Transpiration is the loss of water vapour; the transpiration stream is the resulting upward flow of water.

1
Water evaporates from the moist walls of mesophyll cells into the air spaces of the leaf.
2
Water vapour then diffuses out through open stomata down a gradient into the drier atmosphere.
3
Loss of water from the cell walls pulls more water from adjacent cells and from the xylem, creating tension, or negative pressure, in the xylem.
4
Hydrogen bonds between water molecules give cohesion, so the water column is pulled up as an unbroken chain.
5
Adhesion between water and the hydrophilic walls of xylem vessels helps hold the column up and prevents it from falling back.

Part 2 of 5Why It Matters

Mineral ions
  • Mineral ions The flow of water carries dissolved mineral ions from the roots to the leaves and other parts of the shoot.
Cooling
  • Cooling Evaporation takes heat from the leaf, which helps prevent overheating in sunlight.
Turgor
  • Turgor Water entering cells keeps them turgid, supporting non-woody stems and keeping leaves expanded.
Photosynthesis
  • Photosynthesis Some of the water is used in the light-dependent reactions, although this is a tiny fraction of the water that is transpired.

Part 3 of 5Factors Affecting Transpiration Rate

Factors Affecting Transpiration Rate
Light
  • Light Light opens stomata, so higher light intensity generally increases the rate.
Temperature
  • Temperature Higher temperature increases evaporation and diffusion speed and lowers the humidity of the air, raising the rate.
Humidity
  • Humidity High humidity reduces the water vapour gradient, so transpiration slows.
Wind
  • Wind Moving air carries away water vapour near the leaf and keeps the gradient steep, increasing the rate.
FactorChangeEffect on rate
Light intensityIncreaseIncreases
HumidityIncreaseDecreases
Wind speedIncreaseIncreases

Part 4 of 5Worked Example

Question

  • In a potometer with a capillary tube of radius 0.5 mm, an air bubble moves 45 mm in 10 minutes.
  • Calculate the volume of water taken up per minute.

  1. Cross-section area of tube = π × 0.5² = 0.785 mm².
  2. Volume taken up = 0.785 × 45 = 35.3 mm³.
  3. Rate = 35.3 ÷ 10 = 3.53 mm³ per minute.

Answer Water uptake is approximately 3.5 mm³ per minute.

Part 5 of 5Exam Checklist & Tips

2.7.5 – Key Points for the Exam

  • Transpiration The loss of water vapour from the aerial parts of a plant, mainly through stomata.
  • Cohesion The attraction between molecules of the same substance, such as water held by hydrogen bonds.
  • Adhesion The attraction between water and the walls of the xylem.
  • Potometer Apparatus used to estimate water uptake of a cut shoot.
  • Transpiration pull The tension in the xylem generated by evaporation in the leaf.

Common Mistakes

  • Saying water is pushed up by root pressure; in tall plants the main force is transpiration pull.
  • Writing that cohesion is between water and xylem walls; that is adhesion.
  • Forgetting that stomata must be open for most transpiration to occur.
  • Stating that high humidity increases transpiration.

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